公开(公告)号：US20150026668A1

公开(公告)日：2015-01-22

申请号：US13942944

申请日：2013-07-16

申请人： Jens Boening ,  Karsten Schmidt ,  Xiaohui Xue ,  Christian Mathis

发明人： Jens Boening ,  Karsten Schmidt ,  Xiaohui Xue ,  Christian Mathis

IPC分类号： G06F9/45

CPC分类号： G06F8/51 ,  G06F17/30563

摘要： A system and method for generating a database management system (DBMS) internal program language function from a foreign program language function. The method includes loading the foreign function into a DBMS server, an internal program module unit, examining the foreign function to determine whether the foreign function is annotated for translation, if annotated determining if the foreign function is translated into an internal program language function and executing the internal program language function, else compiling the foreign function into an internal function. If the foreign function is not annotated for translation, executing the foreign program language function. The DBMS system includes a parsing unit, a type inference unit, a normalizer unit, a serializer unit, and a deployer unit. A computer readable medium is also disclosed.

摘要翻译： 一种用于从外部程序语言功能生成数据库管理系统（DBMS）内部程序语言功能的系统和方法。 该方法包括将外部函数加载到DBMS服务器，内部程序模块单元中，检查外部函数以确定外部函数是否被注释用于翻译，如果注释确定外部函数是否被转换为内部程序语言函数并执行 内部程序语言功能，否则将外部函数编译成内部函数。 如果外部函数未注释用于翻译，则执行外部程序语言功能。 DBMS系统包括解析单元，类型推断单元，归一化单元，串行器单元和部署单元。 还公开了一种计算机可读介质。

公开(公告)号：US08746191B2

公开(公告)日：2014-06-10

申请号：US13423074

申请日：2012-03-16

申请人： Jörg Elfner ,  Fevronia Konstantinidou ,  Karsten Schmidt ,  Michael Raffenberg

发明人： Jörg Elfner ,  Fevronia Konstantinidou ,  Karsten Schmidt ,  Michael Raffenberg

IPC分类号： F02B25/00 ,  F02B33/00 ,  F02B33/04 ,  F02M35/10 ,  F02B43/00

CPC分类号： F02B25/20 ,  F02M35/1019 ,  F02M35/108

摘要： A two-stroke engine has an intake arrangement including a carburetor. An intake channel divides into a mixture channel and an air channel. An intermediate flange is mounted between the carburetor and the cylinder. This flange has a carburetor connecting surface, which faces toward the carburetor, and a cylinder connecting surface which faces toward the cylinder. Referred to the flow direction in the intake channel, the air channel divides upstream of the cylinder connecting surface into two branches. The first branch has a first longitudinal center axis and the second branch has a second longitudinal center axis. The intersect points of the center axes with the connecting surfaces are mutually connected via an imaginary connecting line in the carburetor connecting surface or the cylinder connecting surface. The two connecting lines conjointly define an angle β in the carburetor connecting surface with this angle being greater than 0°.

摘要翻译： 二冲程发动机具有包括化油器的进气装置。 进气通道分为混合通道和空气通道。 中间法兰安装在化油器和气缸之间。 该法兰具有朝向化油器的化油器连接表面和朝向气缸的气缸连接表面。 参考进气通道中的流动方向，空气通道将气缸连接表面的上游分为两个分支。 第一分支具有第一纵向中心轴线，第二分支具有第二纵向中心轴线。 中心轴与连接面的交点经由化油器连接面或气缸连接面的假想连接线相互连接。 两条连接线共同定义一个角度＆bgr; 在化油器连接面上，该角度大于0°。

公开(公告)号：US20140057316A1

公开(公告)日：2014-02-27

申请号：US13813804

申请日：2011-02-02

申请人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

发明人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

IPC分类号： C12N9/10

CPC分类号： C12N9/1081 ,  C12Y204/99003

摘要： The present invention relates to a method for the production and purification of a sialyltransferase polypeptide, in particular a N-Acetylgalactosamine (Gal NAc)-α-2,6-sialyltransferase I (ST6GalNAcI) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a Chinese Hamster Ovary (CHO) cell and purifying the polypeptide with a combination of chromatography steps. The method results in high yield of sialyltransferase polypeptide which is highly pure and active. The obtained sialyltransferase, especially ST6GalNAcI, can be employed for the glycosylation of therapeutic proteins such as G-CSF.

摘要翻译： 本发明涉及生产和纯化唾液酸转移酶多肽，特别是N-乙酰半乳糖胺（Gal NAc）-al-2,6-sialyltransferase I（ST6GalNAcI）多肽的方法。 该方法包括在中国仓鼠卵巢（CHO）细胞中产生唾液酸转移酶多肽的步骤，并用色谱步骤的组合纯化多肽。 该方法导致高纯度和活性的唾液酸转移酶多肽的高产量。 获得的唾液酸转移酶，特别是ST6GalNAcI可用于治疗性蛋白质如G-CSF的糖基化。

公开(公告)号：US20070061686A1

公开(公告)日：2007-03-15

申请号：US11499503

申请日：2006-08-04

申请人： Eberhard Boehl ,  Gunter Seydel ,  Karsten Schmidt-Grethe

发明人： Eberhard Boehl ,  Gunter Seydel ,  Karsten Schmidt-Grethe

IPC分类号： H03M13/00

CPC分类号： G11C29/02 ,  G11C29/024

摘要： For checking an address decoder of a data memory, from a record of addresses of the data memory, designated as base addresses, one after another each base address is selected, and the following steps are executed for the respectively selected base address: a) determining the content of the base address; and b) selecting an address having the Hamming distance 1 from the base address, designated as the Hamming address; and then: c) changing the content of the Hamming address selected in step b); and then: d) reading the base address and detecting an error of the address decoder if the content read differs from that determined in step a); and e) recovering the content of the Hamming address.

摘要翻译： 为了检查数据存储器的地址解码器，从指定为基地址的数据存储器的地址记录中，选择一个接一个每个基地址，并且为所选择的基地址执行以下步骤：a）确定 基地址的内容; 以及b）从基地址中选择具有汉明距离1的地址，指定为汉明地址; 然后：c）改变在步骤b）中选择的汉明地址的内容; 然后：d）如果内容读取与步骤a）中确定的内容读取不同，则读取基地址并检测地址解码器的错误; 和e）恢复汉明地址的内容。

公开(公告)号：US09222081B2

公开(公告)日：2015-12-29

申请号：US13813804

申请日：2011-08-02

申请人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

发明人： Axel Angermann ,  Christian Scheckermann ,  Karsten Schmidt

IPC分类号： C12P21/06 ,  C12N9/10

CPC分类号： C12N9/1081 ,  C12Y204/99003

摘要： The present invention relates to a method for the production and purification of a sialyltransferase polypeptide, in particular a N-Acetylgalactosamine (Gal NAc)-α-2,6-sialyltransferase I (ST6GalNAcI) polypeptide. The method comprises the steps of producing the sialyltransferase polypeptide in a Chinese Hamster Ovary (CHO) cell and purifying the polypeptide with a combination of chromatography steps. The method results in high yield of sialyltransferase polypeptide which is highly pure and active. The obtained sialyltransferase, especially ST6GalNAcI, can be employed for the glycosylation of therapeutic proteins such as G-CSF.

摘要翻译： 本发明涉及唾液酸转移酶多肽，特别是N-乙酰半乳糖胺（Gal NAc）-α-2,6-唾液酸转移酶I（ST6GalNAcI）多肽的生产和纯化方法。 该方法包括在中国仓鼠卵巢（CHO）细胞中产生唾液酸转移酶多肽的步骤，并用色谱步骤的组合纯化多肽。 该方法导致高纯度和活性的唾液酸转移酶多肽的高产量。 获得的唾液酸转移酶，特别是ST6GalNAcI可用于治疗性蛋白质如G-CSF的糖基化。

公开(公告)号：US20120276548A1

公开(公告)日：2012-11-01

申请号：US13456179

申请日：2012-04-25

申请人： Karsten Schmidt

发明人： Karsten Schmidt

IPC分类号： C07K14/44 ,  C12N9/96 ,  G01N21/64 ,  C12N1/21 ,  C12N5/10 ,  C12N1/15 ,  C12N1/19 ,  C12Q1/68 ,  G01N33/53 ,  G01N21/76 ,  C07K14/00 ,  C07K16/00 ,  C12N15/62 ,  C07K1/107 ,  C07K19/00 ,  C12N15/63

CPC分类号： G01N33/5308 ,  C07K14/44 ,  Y02A50/55

摘要： The technology relates in part to multimer conjugates comprising a scaffold linked to two or more polypeptides that specifically interact with a nucleic acid containing beta-D-glucosyl-hydroxymethylcytosine or beta-D-glucosyl-hydroxymethyluracil. The scaffold can be chosen from an antibody, an antibody fragment, a multimerized binding partner that interacts with a binding partner counterpart in each of the polypeptides, a polymer, and a polyfunctional molecule. The polypeptides can be from a kinetoplastid flagellate organism and may comprise a full-length native or modified protein or a fragment thereof that specifically interacts with the beta-D-glucosyl-hydroxymethylcytosine and/or the beta-D-glucosyl-hydroxymethyluracil in the nucleic acid. The conjugates provided herein can be used to detect the presence, absence or amount of beta-D-glucosyl-hydroxymethylcytosine and/or beta-D-glucosyl-hydroxymethyluracil-containing nucleic acid in a sample.

摘要翻译： 该技术部分涉及包含与两个或更多个与含有β-D-葡糖基 - 羟基甲基胞嘧啶或β-D-葡糖基 - 羟基甲基尿嘧啶核酸特异性相互作用的多肽的支架的多聚体缀合物。 支架可以选自抗体，抗体片段，与多肽，聚合物和多官能分子中的每一个中的结合配偶体对应物相互作用的多聚体结合配偶体。 多肽可以来自动粒细胞鞭毛虫生物，并且可以包含与核酸中的β-D-葡糖基 - 羟基甲基胞嘧啶和/或β-D-葡糖基 - 羟基甲基尿嘧啶特异性相互作用的全长天然或修饰的蛋白质或其片段 酸。 本文提供的缀合物可用于检测样品中β-D-葡糖基 - 羟甲基胞嘧啶和/或β-D-葡糖基 - 羟甲基尿嘧啶核酸的存在，不存在或数量。

公开(公告)号：US20120185154A1

公开(公告)日：2012-07-19

申请号：US13351567

申请日：2012-01-17

申请人： Robert Böker ,  Karsten Schmidt

发明人： Robert Böker ,  Karsten Schmidt

IPC分类号： F02D28/00 ,  F02D41/30

CPC分类号： F02P1/04 ,  B25F5/00 ,  F02D2400/06 ,  F02P1/086 ,  F02P17/12 ,  F02P2017/121

摘要： A handheld work apparatus has a combustion engine with an ignition unit, a unit for supplying fuel and a device to determine the rotational speed (n). The ignition unit includes a spark plug, a coil and a pole group. The pole group and the coil are moved in dependence on the rotational movement of a crankshaft of the combustion engine, whereby a ignition voltage is induced in the coil. The work apparatus has an open loop control which controls the ignition time (ZZP). A diagnostic unit for damage diagnosis is detachably connected to the work apparatus. For damage diagnosis, the diagnostic unit captures the course of the induced ignition voltage over at least one engine cycle when the combustion engine is running and damage is determined on the basis of the course of the captured voltage.

摘要翻译： 手持式作业装置具有内燃机，其具有点火单元，用于供给燃料的单元和确定转速（n）的装置。 点火单元包括火花塞，线圈和极组。 磁极组和线圈根据内燃机的曲轴的旋转运动而移动，由此在线圈中产生点火电压。 工作装置具有控制点火时间（ZZP）的开环控制。 用于损伤诊断的诊断单元可拆卸地连接到作业装置。 对于损伤诊断，当内燃机运行时，诊断单元捕获至少一个发动机循环的感应点火电压的过程，并且基于捕获电压的过程确定损坏。

公开(公告)号：US20100126467A1

公开(公告)日：2010-05-27

申请号：US12591670

申请日：2009-11-27

申请人： Karsten Schmidt ,  Andreas Hågele

发明人： Karsten Schmidt ,  Andreas Hågele

IPC分类号： F02M7/12

CPC分类号： F02M7/12 ,  F02M1/04 ,  F02M17/04

摘要： The invention relates to a fuel-metering arrangement for the mixture-forming unit (2) of an internal combustion engine (10) in a portable handheld work apparatus. The mixture-forming unit comprises a fuel store (30), a switchable valve (40) and a fuel channel (20) which opens into an intake region of the engine (10). The valve (40) has an inflow port (41) which is connected to the fuel store (30) and an outflow port (42) which is connected to the fuel channel (20). A fuel column is mechanically moved by the switching of the valve. In order to compensate for this fuel column, the outflow port (42) is connected via a bypass channel (45) to the inflow port (41) and a flow valve (46) is mounted in the bypass channel (45). The flow valve (46) opens in flow direction (48) from the outflow port (42) to the inflow port (41) and blocks in the direction opposite thereto.

摘要翻译： 本发明涉及一种用于便携式手持式工作装置中的内燃机（10）的混合物形成单元（2）的燃料计量装置。 混合物形成单元包括燃料储存器（30），可切换阀（40）和通向发动机（10）的进气区域的燃料通道（20）。 阀（40）具有连接到燃料存储器（30）的流入口（41）和连接到燃料通道（20）的流出口（42）。 通过阀的切换机械地移动燃料柱。 为了补偿该燃料柱，流出口42通过旁路通路45与流入口41连接，并且在旁通流路45内安装有流量阀46。 流量阀（46）从流出口（42）向流入口（41）沿流动方向（48）开口，并沿与其相反的方向阻塞。

公开(公告)号：US5685743A

公开(公告)日：1997-11-11

申请号：US554050

申请日：1995-11-06

申请人： Karsten Schmidt ,  Werner Moritz ,  Reinhard Pusch

发明人： Karsten Schmidt ,  Werner Moritz ,  Reinhard Pusch

IPC分类号： H01R4/24 ,  H01R13/506 ,  H01R13/627 ,  H01R13/66 ,  H01R43/18 ,  H01R13/502

CPC分类号： H01R13/506 ,  H01R13/6272 ,  H01R13/66 ,  H01R2201/26 ,  H01R4/2466 ,  H01R43/18

摘要： A connector housing for an air bag connector and a method for contacting an air bag connector. A connector housing having at least two sections is provided with locking elements for a pre-lock-in position and a final lock-in position. In the pre-lock-in position, both sections of the housing are arranged apart from one another and the pre-lock-in position is localized by means of a spacer to maintain the pre-lock-in position maintain at a predetermined distance so that electric conductors can be introduced into the connector housing. The spacer ensures that the connector housing is not inadvertently pressed from the pre-lock-in position to the final lock-in position. The connector housing can be pressed together for an insulation-piercing contact in the connector housing, and locked in the final lock-in position. The connector housing is furthermore provided with a belt arrangement, with the spacer being of a one-piece design with one of the elements of the belt arrangement. When the connector housing is pressed together to engage the line with the contacts, the belt arrangements are cut away so that the spacer is also removed with the belt arrangements.

摘要翻译： 用于气囊连接器的连接器壳体和用于接触气囊连接器的方法。 具有至少两个部分的连接器壳体设置有用于预锁定位置和最终锁定位置的锁定元件。 在预锁定位置，壳体的两个部分彼此分开地布置，并且预锁定位置通过间隔件定位以保持预锁定位置保持在预定距离，因此 可以将电导体引入连接器壳体。 间隔件确保连接器壳体不会被无意地从预锁定位置压到最终锁定位置。 连接器壳体可以被压在一起用于连接器壳体中的绝缘穿刺接触件，并被锁定在最终的锁定位置。 连接器壳体还设置有带装置，其中间隔件是带状装置的一个元件的一体式设计。 当连接器壳体被压在一起以与接触件接合时，皮带装置被切除，使得间隔件也与皮带装置一起被移除。

公开(公告)号：US08951736B2

公开(公告)日：2015-02-10

申请号：US13456179

申请日：2012-04-25

申请人： Karsten Schmidt

发明人： Karsten Schmidt

IPC分类号： C12Q1/68 ,  G01N33/566 ,  G01N33/563 ,  G01N33/53 ,  C07K14/44

CPC分类号： G01N33/5308 ,  C07K14/44 ,  Y02A50/55

摘要： The technology relates in part to multimer conjugates comprising a scaffold linked to two or more polypeptides that specifically interact with a nucleic acid containing beta-D-glucosyl-hydroxymethylcytosine or beta-D-glucosyl-hydroxymethyluracil. The scaffold can be chosen from an antibody, an antibody fragment, a multimerized binding partner that interacts with a binding partner counterpart in each of the polypeptides, a polymer, and a polyfunctional molecule. The polypeptides can be from a kinetoplastid flagellate organism and may comprise a full-length native or modified protein or a fragment thereof that specifically interacts with the beta-D-glucosyl-hydroxymethylcytosine and/or the beta-D-glucosyl-hydroxymethyluracil in the nucleic acid. The conjugates provided herein can be used to detect the presence, absence or amount of beta-D-glucosyl-hydroxymethylcytosine and/or beta-D-glucosyl-hydroxymethyluracil-containing nucleic acid in a sample.

摘要翻译： 该技术部分涉及包含与两个或更多个与含有β-D-葡糖基 - 羟基甲基胞嘧啶或β-D-葡糖基 - 羟基甲基尿嘧啶核酸特异性相互作用的多肽的支架的多聚体缀合物。 支架可以选自抗体，抗体片段，与多肽，聚合物和多官能分子中的每一个中的结合配偶体对应物相互作用的多聚体结合配偶体。 多肽可以来自动粒细胞鞭毛虫生物，并且可以包含与核酸中的β-D-葡糖基 - 羟基甲基胞嘧啶和/或β-D-葡糖基 - 羟基甲基尿嘧啶特异性相互作用的全长天然或修饰的蛋白质或其片段 酸。 本文提供的缀合物可用于检测样品中β-D-葡糖基 - 羟甲基胞嘧啶和/或β-D-葡糖基 - 羟甲基尿嘧啶核酸的存在，不存在或数量。