公开(公告)号：US20080085859A1

公开(公告)日：2008-04-10

申请号：US10564446

申请日：2004-03-29

申请人： Kentaro Hanada ,  Masahiro Nishijima ,  Keigo Kumagai

发明人： Kentaro Hanada ,  Masahiro Nishijima ,  Keigo Kumagai

IPC分类号： A61K38/00 ,  A61P43/00 ,  C07H21/00 ,  G01N33/00

CPC分类号： C07K14/47 ,  A61K38/00

摘要： A drug for promoting ceramide transport contains a protein having the amino acid sequence of SEQ ID NO:1 as an effective component; the base sequence of SEQ ID NO:3; a method of measuring an activity for promoting ceramide release comprises mixing a lipid membrane containing ceramide with a drug promoting ceramide release, centrifuging the thus obtained mixture to give a supernatant and measuring the ceramide content in the supernatant; and a method of measuring an activity for promoting intermembrane ceramide transfer comprises mixing a receiving membrane, a drug for promoting ceramide transport and a donating membrane containing ceramide, adding a selective membrane aggregating agent thereto, then separating the receiving membrane from the donating membrane and measuring ceramide contents in the receiving membrane and in the donating membrane. Thus, it is possible to provide a drug for promoting ceramide transport, a base sequence for producing the drug, relevant a method of measuring an activity for promoting ceramide release and a method of measuring an activity for promoting intermembrane ceramide transfer.

摘要翻译： 用于促进神经酰胺转运的药物含有具有SEQ ID NO：1的氨基酸序列作为有效成分的蛋白质; SEQ ID NO：3的碱基序列; 测定促进神经酰胺释放的活性的方法包括将含有神经酰胺的脂质膜与药物促进神经酰胺释放混合，离心所得混合物，得到上清液并测定上清液中的神经酰胺含量; 测定促进膜间神经酰胺转移的活性的方法包括将接收膜，促进神经酰胺转运用药物和含有神经酰胺的供体膜混合，向其中加入选择性膜聚集剂，然后将接收膜与供体膜分离并测量 神经酰胺含量在接受膜和捐赠膜中。 因此，可以提供用于促进神经酰胺转运的药物，用于制备药物的碱基序列，相关的测量促进神经酰胺释放的活性的方法和测量促进膜间神经酰胺转移的活性的方法。

公开(公告)号：US06888015B2

公开(公告)日：2005-05-03

申请号：US10472379

申请日：2002-04-19

申请人： Shu Kobayashi ,  Kentaro Hanada

发明人： Shu Kobayashi ,  Kentaro Hanada

IPC分类号： A61K31/165 ,  A61P43/00 ,  C07C233/18 ,  C07C233/05

CPC分类号： C07C233/18 ,  A61K31/165

摘要： A inhibitor of sphingolipids synthesis comprising a compound represented by general formula A. Desirably, a novel inhibitor of sphingolipids synthesis comprising (1R, 3R) N-(3-hydroxy-1-hydroxymethyl-3-phenylpropyl) alkaneamide, wherein carbon number of alkanoyl group is 8-16. (in the formula, X is an aliphatic alkyl group, desirably is an aliphatic alkyl group having 7 to 15 carbon atoms).

摘要翻译： 含有由通式A表示的化合物的鞘脂合成抑制剂。理想地，包含（1R，3R）N-（3-羟基-1-羟甲基-3-苯基丙基）烷酰胺的新型鞘脂合成抑制剂，其中烷酰基 组是8-16。 （式中，X为脂肪族烷基，优选碳原子数为7〜15的脂肪族烷基）。