公开(公告)号：US20180051332A9

公开(公告)日：2018-02-22

申请号：US14119859

申请日：2012-05-29

申请人： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

发明人： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  G01N27/327 ,  G01N33/54313 ,  G01N33/5438 ,  C12Q2527/119 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2549/126

摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US09926596B2

公开(公告)日：2018-03-27

申请号：US14119859

申请日：2012-05-29

申请人： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

发明人： Hesaam Esfandyarpour ,  Kosar Baghbani Parizi ,  Mark F. Oldham ,  Eric S. Nordman ,  Richard T. Reel ,  Susanne Baumhueter ,  Cheryl Heiner ,  Frank Lee

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6874 ,  G01N27/327 ,  G01N33/54313 ,  G01N33/5438 ,  C12Q2527/119 ,  C12Q2563/116 ,  C12Q2565/607 ,  C12Q2549/126

摘要： The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

公开(公告)号：US09187783B2

公开(公告)日：2015-11-17

申请号：US13824129

申请日：2011-10-04

申请人： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Eric S. Nordman ,  Kosar Baghbani Parizi

发明人： Hesaam Esfandyarpour ,  Mark F. Oldham ,  Eric S. Nordman ,  Kosar Baghbani Parizi

IPC分类号： C12Q1/68 ,  G01N27/414

CPC分类号： C12Q1/6874 ,  B01L3/502738 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2300/089 ,  B01L2300/12 ,  B01L2300/123 ,  B01L2400/0415 ,  B01L2400/0418 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0655 ,  B03C1/286 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C5/022 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6848 ,  C12Q1/6853 ,  C12Q1/6869 ,  C40B60/10 ,  G01N1/40 ,  G01N27/4145 ,  G01N27/4146 ,  G01N33/5438 ,  G01N2001/4038 ,  H01L29/00 ,  C12Q2523/307 ,  C12Q2563/143 ,  C12Q2565/543 ,  C12Q2563/116 ,  C12Q2565/607

摘要： A method comprises magnetically holding a bead carrying biological material (e.g., nucleic acid, which may be in the form of DNA fragments or amplified DNA) in a specific location of a substrate, and applying an electric field local to the bead to isolate the biological material or products or byproducts of reactions of the biological material. For example, the bead is isolated from other beads having associated biological material. The electric field in various embodiments concentrates reagents for an amplification or sequencing reaction, and/or concentrates and isolates detectable reaction by-products. For example, by isolating nucleic acids around individual beads, the electric field can allow for clonal amplification, as an alternative to emulsion PCR. In other embodiments, the electric field isolates a nanosensor proximate to the bead, to facilitate detection of at least one of local pH change, local conductivity change, local charge concentration change and local heat. The beads may be trapped in the form of an array of localized magnetic field regions.

摘要翻译： 一种方法包括在衬底的特定位置磁性地保持携带生物材料（例如，核酸，其可以是DNA片段或扩增的DNA的形式）的珠，并施加局部于珠的电场以分离生物 材料或生物材料的反应产物或副产物。 例如，珠粒与具有相关生物材料的其它珠粒分离。 各种实施方案中的电场浓缩用于扩增或测序反应的试剂，和/或浓缩和分离可检测的反应副产物。 例如，通过分离单个珠周围的核酸，电场可以允许克隆扩增，作为乳液PCR的替代。 在其他实施例中，电场将纳米传感器隔离到珠子附近，以便于检测局部pH变化，局部电导率变化，局部电荷浓度变化和局部热中的至少一种。 珠可以以局部磁场区域阵列的形式被捕获。

公开(公告)号：US09184099B2

公开(公告)日：2015-11-10

申请号：US13252315

申请日：2011-10-04

申请人： Kosar Baghbani-Parizi ,  Yoshio Nishi ,  Hesaam Esfandyarpour

发明人： Kosar Baghbani-Parizi ,  Yoshio Nishi ,  Hesaam Esfandyarpour

IPC分类号： G01N27/414 ,  H01L21/02 ,  H01L29/66 ,  H01L21/84 ,  B82Y15/00 ,  H01L27/12 ,  B01L3/00

CPC分类号： G01N27/4145 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/12 ,  B82Y15/00 ,  H01L21/26513 ,  H01L21/3065 ,  H01L21/84 ,  H01L27/1203

摘要： A sensing apparatus for sensing target materials including biological or chemical molecules in a fluid. One such apparatus includes a semiconductor-on-insulator (SOI) structure having an electrically-insulating layer, a fluidic channel supported by the SOI structure and configured and arranged to receive and pass a fluid including the target materials, and a semiconductor device including at least three electrically-contiguous semiconductor regions doped to exhibit a common polarity. The semiconductor regions include a sandwiched region sandwiched between two of the other semiconductor regions, and configured and arranged adjacent to the fluidic channel with a surface directed toward the fluidic channel for coupling to the target materials in the fluidic channel, and further arranged for responding to a bias voltage. The sensing apparatus also includes an amplification circuit in or on the SOI and that is arranged to facilitate sensing of the target material near the fluidic channel.

摘要翻译： 一种感测装置，用于感测流体中包括生物或化学分子的目标材料。 一种这样的设备包括具有电绝缘层的绝缘体上半导体（SOI）结构，由SOI结构支撑的流体通道，并且被构造和布置成接收和通过包括目标材料的流体，以及包括在 至少三个电连续的半导体区被掺杂以表现出共同的极性。 半导体区域包括夹在两个其它半导体区域之间的夹层区域，并且被配置和布置成与流体通道相邻，其表面指向流体通道，用于耦合到流体通道中的目标材料，并且还被布置为响应于 偏置电压。 感测装置还包括在SOI中或SOI上的放大电路，并且被布置成便于感测流体通道附近的目标材料。

公开(公告)号：US20120138460A1

公开(公告)日：2012-06-07

申请号：US13252315

申请日：2011-10-04

申请人： Kosar Baghbani-Parizi ,  Yoshio Nishi ,  Hesaam Esfandyarpour

发明人： Kosar Baghbani-Parizi ,  Yoshio Nishi ,  Hesaam Esfandyarpour

IPC分类号： G01N27/414 ,  H01L21/02 ,  H01L29/66

CPC分类号： G01N27/4145 ,  B01L3/502715 ,  B01L3/502753 ,  B01L2200/12 ,  B01L2300/0645 ,  B01L2300/12 ,  B82Y15/00 ,  H01L21/26513 ,  H01L21/3065 ,  H01L21/84 ,  H01L27/1203

摘要： A sensing apparatus for sensing target materials including biological or chemical molecules in a fluid. One such apparatus includes a semiconductor-on-insulator (SOI) structure having an electrically-insulating layer, a fluidic channel supported by the SOI structure and configured and arranged to receive and pass a fluid including the target materials, and a semiconductor device including at least three electrically-contiguous semiconductor regions doped to exhibit a common polarity. The semiconductor regions include a sandwiched region sandwiched between two of the other semiconductor regions, and configured and arranged adjacent to the fluidic channel with a surface directed toward the fluidic channel for coupling to the target materials in the fluidic channel, and further arranged for responding to a bias voltage. The sensing apparatus also includes an amplification circuit in or on the SOI and that is arranged to facilitate sensing of the target material near the fluidic channel.

摘要翻译： 一种感测装置，用于感测流体中包括生物或化学分子的目标材料。 一种这样的设备包括具有电绝缘层的绝缘体上半导体（SOI）结构，由SOI结构支撑的流体通道，并且被构造和布置成接收和通过包括目标材料的流体，以及包括在 至少三个电连续的半导体区被掺杂以表现出共同的极性。 半导体区域包括夹在两个其它半导体区域之间的夹层区域，并且被配置和布置成与流体通道相邻，其表面指向流体通道，用于耦合到流体通道中的目标材料，并且还被布置为响应于 偏置电压。 感测装置还包括在SOI中或SOI上的放大电路，并且被布置成便于感测流体通道附近的目标材料。