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    Enzyme conjugate prepared with insoluble nonoparticle
    2.
    发明授权
    Enzyme conjugate prepared with insoluble nonoparticle 失效
    用不溶性非颗粒制备的酶偶联物

    公开(公告)号：US5405752A

    公开(公告)日：1995-04-11

    申请号：US185213

    申请日：1994-01-14

    申请人： Kurt G. I. Nilsson

    发明人： Kurt G. I. Nilsson

    IPC分类号： G01N33/543 ,  G01N33/58 ,  G01N33/53

    CPC分类号： G01N33/54313 ,  G01N33/581 ,  G01N33/585 ,  Y10S436/829

    摘要： The invention relates to a reagent consisting of at least one enzyme and at least one other substance, which are covalently or noncovalently bound to a particle, which is smaller than or is equal to 1000 Angstrom in diameter. The invention also relates to method and use of the reagent for determination or studies of a cell or a virus or another component in a sample. According to the invention, these determinations are made with for example some ELISA-technique (competitive, sandwich, etc), employing the reagent preferably in the form of a suspension in buffered water, instead of and in the same way as described for soluble enzyme conjugates. The substance can be antibody, a lectin, avidin or an antigen, the enzyme can be for example peroxidase, alcaline phosphatase, and the particle can be an inorganic or an organic polymeric compound or a combination thereof, as, for example, tresyl-activated glycerylpropyl-silica.

    摘要翻译： 本发明涉及由至少一种酶和至少一种其它物质组成的试剂，其与颗粒共价或非共价结合，其直径小于或等于1000埃。 本发明还涉及用于测定或研究样品中的细胞或病毒或其它成分的试剂的方法和用途。 根据本发明，通过例如一些ELISA技术（竞争性，夹心等）进行这些测定，其中使用试剂优选以缓冲水中的悬浮液的形式，而不是以与可溶性酶所述相同的方式 共轭物 该物质可以是抗体，凝集素，抗生物素蛋白或抗原，酶可以是例如过氧化物酶，碱性磷酸酶，并且该颗粒可以是无机或有机高分子化合物或其组合，例如，甲酚基活化 甘油基丙基二氧化硅。

    Method and product for blood treatment and purification
    3.
    发明授权
    Method and product for blood treatment and purification 有权

    公开(公告)号：US09675746B2

    公开(公告)日：2017-06-13

    申请号：US13501640

    申请日：2010-10-18

    申请人： Kurt G. I. Nilsson

    发明人： Kurt G. I. Nilsson

    IPC分类号： A01N63/00 ,  C12N5/00 ,  C12N5/02 ,  A61M1/34

    CPC分类号： A61M1/3472 ,  A61M1/3486 ,  A61M1/3493 ,  A61M1/362

    摘要： Extracorporeal blood treatment is performed daily on a large number of patients by use of for example a dialysis filter, plasma filter or a centrifuge. The purpose of the treatment is to separate minor components and molecules in a liquid or in the blood from larger ones, for example in connection with different disease conditions or with a view to extracting blood plasma, target substances such as blood components or molecules from for example blood donors. According to the present invention two separation steps are used together with a solution or a suspension containing at least one component, which specifically may bind to the component or the blood component to be specifically reduced, or to be refined/extracted during the treatment or during the blood treatment.

    METHOD AND PRODUCT FOR BLOOD TREATMENT AND PURIFICATION
    5.
    发明申请
    METHOD AND PRODUCT FOR BLOOD TREATMENT AND PURIFICATION 有权
    用于血液处理和净化的方法和产品

    公开(公告)号：US20120230969A1

    公开(公告)日：2012-09-13

    申请号：US13501640

    申请日：2010-10-18

    申请人： Kurt G. I. Nilsson

    发明人： Kurt G. I. Nilsson

    IPC分类号： A61K35/18 ,  C12N5/078

    CPC分类号： A61M1/3472 ,  A61M1/3486 ,  A61M1/3493 ,  A61M1/362

    摘要： Extracorporeal blood treatment is performed daily on a large number of patients by use of for example a dialysis filter, plasma filter or a centrifuge. The purpose of the treatment is to separate minor components and molecules in a liquid or in the blood from larger ones, for example in connection with different disease conditions or with a view to extracting blood plasma, target substances such as blood components or molecules from for example blood donors. According to the present invention two separation steps are used together with a solution or a suspension containing at least one component, which specifically may bind to the component or the blood component to be specifically reduced, or to be refined/extracted during the treatment or during the blood treatment.

    摘要翻译： 通过使用例如透析过滤器，血浆过滤器或离心机，在大量患者上每天进行体外血液处理。 治疗的目的是将液体或血液中的次要组分和分子从较大的组分分离出来，例如与不同的疾病状况或为了提取血浆，目标物质如血液成分或分子，从 示例献血者。 根据本发明，两个分离步骤与含有至少一种组分的溶液或悬浮液一起使用，其特异性可以结合待特异性还原的组分或血液组分，或在治疗期间或在治疗期间精细/提取 血液处理。

    Amino-deoxy-disaccharides and amino-deoxy-oligosaccharides
    6.
    发明授权
    Amino-deoxy-disaccharides and amino-deoxy-oligosaccharides 失效
    氨基脱氧二糖和氨基脱氧低聚糖

    公开(公告)号：US5936075A

    公开(公告)日：1999-08-10

    申请号：US474173

    申请日：1995-06-07

    申请人： Kurt G. I. Nilsson

    发明人： Kurt G. I. Nilsson

    IPC分类号： C07H15/14 ,  C07H15/18 ,  C07H15/00 ,  C07H17/00

    CPC分类号： C07H15/14 ,  C07H15/18

    摘要： Synthesis of an amino-disaccharide, amino-oligosaccharide or a derivative thereof, characterized in that a monosaccharide, a disaccharide, an oligosaccharide, a glycoside or a derivative thereof, in the presence of a glycosidase as catalyst, is reacted with an amino-deoxy-saccharide or a derivative thereof, and that the amino-saccharide is isolated from the product mixture directly or after chemical/enzymatic modification.

    摘要翻译： 氨基 - 二糖或其衍生物的合成，其特征在于在糖苷酶作为催化剂存在下，将单糖，二糖，寡糖，糖苷或其衍生物与氨基脱氧 糖或其衍生物，并且直接或在化学/酶修饰后从产物混合物中分离出氨基糖。

    Method for synthesis of oligosaccharides
    8.
    发明授权
    Method for synthesis of oligosaccharides 失效
    合成寡糖的方法

    公开(公告)号：US5246840A

    公开(公告)日：1993-09-21

    申请号：US603699

    申请日：1990-11-16

    申请人： Kurt G. I. Nilsson

    发明人： Kurt G. I. Nilsson

    IPC分类号： C12P19/04 ,  C12P19/26 ,  C12P19/44

    CPC分类号： C12P19/44 ,  C12P19/04 ,  C12P19/26

    摘要： A method for synthesis of oligosaccharide compounds which either consist of or are fragments or analogs of the carbohydrate part in glycoconjugates is described. The synthesis from donor and acceptor substrates is carried out in that at least one glycosidase and at least one glycosyltransferase are used as catalysts and the oligosaccharide compound is isolated from the reaction mixture.

    摘要翻译： PCT No.PCT / SE89 / 00151 Sec。 371日期1990年11月16日 102（e）日期1990年11月16日PCT 1989年3月22日PCT公布。 出版物WO89 / 09275 1980年10月5日的日期。一种寡糖化合物的合成方法，其由糖缀合物中的碳水化合物部分组成或者是片段或类似物来描述。 由供体和受体底物的合成进行，其中使用至少一种糖苷酶和至少一种糖基转移酶作为催化剂，并且从反应混合物中分离寡糖化合物。

    Process for producing an oligosaccharide compound by using glycosidases from a mollusc
    9.
    发明授权
    Process for producing an oligosaccharide compound by using glycosidases from a mollusc 失效
    通过使用软体动物的糖苷酶制备寡糖化合物的方法

    公开(公告)号：US5372937A

    公开(公告)日：1994-12-13

    申请号：US834575

    申请日：1992-02-18

    申请人： Kurt G. I. Nilsson

    发明人： Kurt G. I. Nilsson

    IPC分类号： C12N9/24 ,  C12P19/12 ,  C12P19/14 ,  C12P19/04 ,  C07H3/06 ,  C12N9/22

    CPC分类号： C12P19/12 ,  C12N9/2402 ,  C12P19/14 ,  C12Y302/01096

    摘要： A method of producing an oligosaccharide compound which either consists of or is a fragment or analog of the carbohydrate part in a glycoconjugate is disclosed which involves reacting(a) at least one oligosaccharide, disaccharide, monosaccharide, or glycoside as donor substance,(b) at least one acceptor substance containing a monosaccharide, disaccharide, oligosaccharide, glycoside, or saccharide analog, and(c) at least one enzyme composition produced from a mollusc and containing E.C. group 3.2 glycosidase, or the glycosidase is at least one glycosidase which has been cloned with recombinant technique and which has at least 70% homology in its amino acid sequence with the corresponding mollusc enzyme, to form the oligosaccharide compound;wherein the oligosaccharide compound contains(i) GlcNAc.beta.1-3Gal.beta. and the glycosidase is N-acetyl-.beta.-D-glucosaminidase,(ii) GlcNAc.beta.1-6Man.alpha. and the glycosidase is N-acetyl-.beta.-D-glucosaminidase,(iii) GlcNAc.beta.1-6Gal.alpha. and the glycosidase is N-acetyl-.beta.-D-glucosaminidase,(iv) GalNAc.beta.1-3Gal.beta. and the glycosidase is N-acetyl-.beta.-D-galactosaminidase,(v) GalNAc.alpha.1-3Gal.alpha. and the glycosidase is N-acetyl-.alpha.-D-galactosaminidase, or(vi) Fuc.alpha.1-6Gal.beta. and the glycosidase is .alpha.-L-fucosidase.

    摘要翻译： PCT No.PCT / SE90 / 00537 Sec。 371日期1992年2月18日 102（e）日期1992年2月18日PCT提交1990年8月17日PCT公布。 出版物WO91 / 02806 公开了一种制备寡糖化合物的方法，其由糖缀合物中的碳水化合物部分的片段或类似物组成或包含，其包括使（a）至少一种寡糖，二糖，单糖或糖苷 作为供体物质，（b）至少一种含有单糖，二糖，寡糖，糖苷或糖类似物的受体物质，以及（c）由软体动物和含有EC3.2糖苷酶或糖苷酶产生的至少一种酶组合物 至少一种已经用重组技术克隆的糖苷酶，并且其氨基酸序列与相应的软体动物酶具有至少70％的同源性以形成寡糖化合物; 其中所述寡糖化合物包含（i）GlcNAcβ1〜3Galβ，所述糖苷酶为N-乙酰基-β-D-葡糖胺酶，（ii）GlcNAcβ1-6Manα和所述糖苷酶为N-乙酰基-β-D-葡糖胺酶 ，（iii）GlcNAcβ1-6Galα和糖苷酶是N-乙酰基-β-D-葡糖胺酶，（iv）GalNAcβ1-3Galβ和糖苷酶是N-乙酰基-β-D-半乳糖胺酶，（v） GalNAcα1-3Galα和糖苷酶是N-乙酰基-α-D-半乳糖苷酶，或（vi）Fucα1-6Galβ，糖苷酶是α-L-岩藻糖苷酶。

    Method of controlling the regioselectivity of glycosidic bonds
    10.
    发明授权
    Method of controlling the regioselectivity of glycosidic bonds 失效
    控制糖苷键区域选择性的方法

    公开(公告)号：US4918009A

    公开(公告)日：1990-04-17

    申请号：US938703

    申请日：1986-12-04

    申请人： Kurt G. I. Nilsson

    发明人： Kurt G. I. Nilsson

    IPC分类号： C07H15/04 ,  C07H3/06 ,  C07H15/203 ,  C12P1/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/14 ,  C12P19/16 ,  C12P19/18 ,  C12P19/20 ,  C12P19/22 ,  C12P19/44

    CPC分类号： C12P19/18 ,  C07H3/06 ,  C12P19/04 ,  C12P19/12 ,  C12P19/14 ,  C12P19/44

    摘要： A method of controlling the regioselectivity of the glycosidic bond between glycosyl donor and glycosyl acceptor in the enzymatic production of an oligosaccharide compound which either consists of or is a fragment or an analog of the cabohydrate part in a glycoconjugate, by reverse hydrolysis or transglycosidation reactions, is described. The synthesis is carried out in that a donor substance which is a mono- or oligosaccharide or a glycoside thereof, is caused to react, in the presence of a glycosidase, with an acceptor substance which is an O-, N-, C- or S-glycoside consisting of a monosaccharide, oligosaccharide or a saccharide analog and at least one aglycon which is O-, N-, C- or S-glycosidically bonded in 1-position, the .alpha. or .beta.-configuration being selected on the glycoside bond between the glycosyl group and the aglycon in the acceptor substance, and the oligosaccharide compound being separated from the reaction mixture.

    摘要翻译： 通过反向水解或反式糖苷化反应，控制糖基供体与糖基受体之间的糖苷键的区域选择性的方法，其中寡糖化合物的酶促生产由糖缀合物中的半合成部分的片段或类似物组成， 被描述。 合成是在糖苷酶的存在下使作为其单糖或寡糖或其糖苷的供体物质与受体物质O-，N-，C-或 由单糖，寡糖或糖类似物组成的S-糖苷和至少一个在1-位上O-，N-，C-或S-糖苷键连接的糖苷配基，在糖苷键上选择α或β-构型 在受体物质之间的糖基和糖苷配基之间，并且寡糖化合物与反应混合物分离。