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公开(公告)号：US08728793B2
公开(公告)日：2014-05-20
申请号：US12172796
申请日：2008-07-14
Applicant: Jeffrey Glenn , Nam-Joon Cho , Curtis W. Frank , Kwang Ho Cheong
Inventor： Jeffrey Glenn , Nam-Joon Cho , Curtis W. Frank , Kwang Ho Cheong
CPC classification number: A61K38/162 , A01N37/46 , A61L2/0082 , A61L29/08 , A61L29/16 , A61L31/08 , A61L31/16 , A61L2300/25 , A61L2300/408
Abstract: The invention features methods and compositions that exploit the ability of amphipathic alpha-helical (AH) peptides to cause disruption of lipid-containing vesicles, such as enveloped viruses, in a size-dependent manner.
Abstract translation: 本发明的特征在于利用两亲性α-螺旋（AH）肽以大小依赖的方式引起含脂质囊泡如包膜病毒破坏的能力的方法和组合物。
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公开(公告)号：US08211712B2
公开(公告)日：2012-07-03
申请号：US11887669
申请日：2006-03-29
Applicant: Nam-Joon Cho , Curtis W. Frank , Jeffrey S. Glenn , Kwang Ho Cheong
Inventor： Nam-Joon Cho , Curtis W. Frank , Jeffrey S. Glenn , Kwang Ho Cheong
IPC: G01N33/543
CPC classification number: G01N33/533 , B82Y5/00 , G01N33/554 , Y10T428/31678
Abstract: The present invention provides a method of producing a planar lipid bilayer on a solid support. With this method, a solution of lipid vesicles is first deposited on the solid support. Next, the lipid vesicles are destabilized by adding an amphipathic peptide solution to the lipid vesicle solution. This destabilization leads to production of a planar lipid bilayer on the solid support. The present invention also provides a supported planar lipid bilayer, where the planar lipid bilayer is made of naturally occurring lipids and the solid support is made of unmodified gold or titanium oxide. Preferably, the supported planar lipid bilayer is continuous. The planar lipid bilayer may be made of any naturally occurring lipid or mixture of lipids, including, but not limited to phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinsitol, cardiolipin, cholesterol, and sphingomyelin.
Abstract translation: 本发明提供了一种在固体支持物上制备平面脂双层的方法。 使用该方法，首先将脂质囊泡溶液沉积在固体支持物上。 接下来，通过向脂质囊泡溶液中加入两亲肽溶液使脂质囊泡不稳定。 这种不稳定性导致在固体支持物上产生平面脂双层。 本发明还提供了一种支撑的平面脂质双层，其中平面脂质双层由天然存在的脂质制成，并且固体支持物由未改性的金或氧化钛制成。 优选地，支撑的平面脂质双层是连续的。 平面脂质双层可以由任何天然存在的脂质或脂质混合物制成，包括但不限于磷脂酰胆碱，磷脂酰乙醇胺，磷脂酰丝氨酸，磷脂酰胰岛素，心磷脂，胆固醇和鞘磷脂。
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3.发明申请METHOD AND APPARATUS FOR DISRUPTING CELLS AND AMPLIFYING NUCLEIC ACIDS USING GOLD NANORODS 有权破坏细胞的方法和装置，使用金纳多糖放大核酸公开(公告)号：US20110212514A1
公开(公告)日：2011-09-01
申请号：US13107677
申请日：2011-05-13
Applicant: Kwang Ho CHEONG , Dong-kee YI , Jeong-gun LEE , Jong-myeon PARK
Inventor： Kwang Ho CHEONG , Dong-kee YI , Jeong-gun LEE , Jong-myeon PARK
Abstract: Provided is a method of disrupting cells comprising adding gold nanorods to a solution containing cells and irradiating the gold nanorods with a laser to disrupt the cells. A method and an apparatus for continuously disrupting cells and amplifying nucleic acids in a single microchamber are also provided, wherein the method comprises introducing a solution containing cells and gold nanorods into a microchamber, irradiating a laser onto the gold nanorods to disrupt the cells, and amplifying a nucleic acid from the disrupted cells in the microchamber.
Abstract translation: 提供一种破坏细胞的方法，包括向含有细胞的溶液中加入金纳米棒并用激光照射金纳米棒以破坏细胞。 还提供了用于在单个微室中连续破坏细胞和扩增核酸的方法和装置，其中所述方法包括将含有细胞和金纳米棒的溶液引入微室中，将激光照射到金纳米棒上以破坏细胞，以及 在微室中从破碎的细胞中扩增核酸。
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4.发明申请METHOD AND APPARATUS FOR DISRUPTING CELLS AND AMPLIFYING NUCLEIC ACIDS USING GOLD NANORODS 有权破坏细胞的方法和装置，使用金纳多糖放大核酸公开(公告)号：US20080171366A1
公开(公告)日：2008-07-17
申请号：US11838550
申请日：2007-08-14
Applicant: Kwang Ho CHEONG , Dong-kee YI , Jeong-gun LEE , Jong-myeon PARK
Inventor： Kwang Ho CHEONG , Dong-kee YI , Jeong-gun LEE , Jong-myeon PARK
Abstract: Provided is a method of disrupting cells comprising adding gold nanorods to a solution containing cells and irradiating the gold nanorods with a laser to disrupt the cells. A method and an apparatus for continuously disrupting cells and amplifying nucleic acids in a single microchamber are also provided, wherein the method comprises introducing a solution containing cells and gold nanorods into a microchamber, irradiating a laser onto the gold nanorods to disrupt the cells, and amplifying a nucleic acid from the disrupted cells in the microchamber.
Abstract translation: 提供一种破坏细胞的方法，包括向含有细胞的溶液中加入金纳米棒并用激光照射金纳米棒以破坏细胞。 还提供了用于在单个微室中连续破坏细胞和扩增核酸的方法和装置，其中所述方法包括将含有细胞和金纳米棒的溶液引入微室中，将激光照射到金纳米棒上以破坏细胞，以及 在微室中从破碎的细胞中扩增核酸。
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公开(公告)号：US20140294838A1
公开(公告)日：2014-10-02
申请号：US14229308
申请日：2014-03-28
Applicant: Mi Young CHO , Powei Lin , Seung Hyun Lee , Kwang Ho Cheong , Young Jun Koh , Christina Yi , Jae Woong Hwang
Inventor： Mi Young CHO , Powei Lin , Seung Hyun Lee , Kwang Ho Cheong , Young Jun Koh , Christina Yi , Jae Woong Hwang
IPC: C07K16/32
CPC classification number: A61K39/39558 , C07K16/2863 , C07K16/32 , C07K2317/24 , C07K2317/31 , C07K2317/34 , C07K2317/565 , C07K2317/622 , C07K2317/64 , C07K2317/92
Abstract: There are provided an anti-c-Met/anti-Her2 bispecific antibody, and a method for preventing and/or treating cancer using the same.
Abstract translation: 提供抗c-Met /抗Her2双特异性抗体，以及使用其的预防和/或治疗癌症的方法。
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Patent Agency Ranking
6.发明授权Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权使用微磁珠和激光快速破坏细胞或病毒的方法和装置公开(公告)号：US07855069B2
公开(公告)日：2010-12-21
申请号：US11253541
申请日：2005-10-19
Applicant: Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
Inventor： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
CPC classification number: C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
Abstract: A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
Abstract translation: 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠将振动有效地转移到细胞中，通过疏水处理芯片的内表面和细胞来解决粗糙表面上的微流体问题 裂解芯片可应用于LOC。
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公开(公告)号：US20090105151A1
公开(公告)日：2009-04-23
申请号：US12172796
申请日：2008-07-14
Applicant: Jeffrey Glenn , Nam-Joon Cho , Curtis W. Frank , Kwang Ho Cheong
Inventor： Jeffrey Glenn , Nam-Joon Cho , Curtis W. Frank , Kwang Ho Cheong
CPC classification number: A61K38/162 , A01N37/46 , A61L2/0082 , A61L29/08 , A61L29/16 , A61L31/08 , A61L31/16 , A61L2300/25 , A61L2300/408
Abstract: The invention features methods and compositions that exploit the ability of amphipathic alpha-helical (AH) peptides to cause disruption of lipid-containing vesicles, such as enveloped viruses, in a size-dependent manner.
Abstract translation: 本发明的特征在于利用两亲性α-螺旋（AH）肽以大小依赖的方式引起含脂质囊泡如包膜病毒破坏的能力的方法和组合物。
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8.发明申请APPARATUS FOR INTRODUCING FLUID INTO MICROFLUIDIC CHIP BY USING CENTRIFUGAL FORCE, A SYSTEM INCLUDING THE APPARATUS, AND A METHOD OF USING THE APPARATUS 有权通过使用离心力将流体引入微流化芯片的装置，包括装置的系统以及使用装置的方法公开(公告)号：US20080072994A1
公开(公告)日：2008-03-27
申请号：US11846794
申请日：2007-08-29
Applicant: Young-rok KIM , Jun-hong MIN , Kak NAMKOONG , Kwang-ho CHEONG , Chang-eun YOO , Ki-woong HAN , Ki-eun KIM
Inventor： Young-rok KIM , Jun-hong MIN , Kak NAMKOONG , Kwang-ho CHEONG , Chang-eun YOO , Ki-woong HAN , Ki-eun KIM
IPC: B65B1/14
CPC classification number: B01L9/527 , B01L3/5021 , B01L3/5027 , B01L2200/027 , B01L2300/0832 , B01L2400/0409 , C12M23/16
Abstract: An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.
Abstract translation: 使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到 引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在 引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于 由旋转产生的离心力。
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9.发明授权Method and apparatus for disrupting cells and amplifying nucleic acids using gold nanorods 有权使用金纳米棒破坏细胞和扩增核酸的方法和装置公开(公告)号：US07972819B2
公开(公告)日：2011-07-05
申请号：US11838550
申请日：2007-08-14
Applicant: Kwang Ho Cheong , Dong-kee Yi , Jeong-gun Lee , Jong-myeon Park
Inventor： Kwang Ho Cheong , Dong-kee Yi , Jeong-gun Lee , Jong-myeon Park
Abstract: Provided is a method of disrupting cells comprising adding gold nanorods to a solution containing cells and irradiating the gold nanorods with a laser to disrupt the cells. A method and an apparatus for continuously disrupting cells and amplifying nucleic acids in a single microchamber are also provided, wherein the method comprises introducing a solution containing cells and gold nanorods into a microchamber, irradiating a laser onto the gold nanorods to disrupt the cells, and amplifying a nucleic acid from the disrupted cells in the microchamber.
Abstract translation: 提供一种破坏细胞的方法，包括向含有细胞的溶液中加入金纳米棒并用激光照射金纳米棒以破坏细胞。 还提供了用于在单个微室中连续破坏细胞和扩增核酸的方法和装置，其中所述方法包括将含有细胞和金纳米棒的溶液引入微室中，将激光照射到金纳米棒上以破坏细胞，以及 在微室中从破碎的细胞中扩增核酸。
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10.发明申请Method and apparatus for the rapid disruption of cells or viruses using micro magnetic beads and laser 有权使用微磁珠和激光快速破坏细胞或病毒的方法和装置公开(公告)号：US20060084165A1
公开(公告)日：2006-04-20
申请号：US11253541
申请日：2005-10-19
Applicant: Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
Inventor： Jeong-gun Lee , Young-nam Kwon , Young-a Kim , Myo-yong Lee , Shin-i Yoo , Yeon-ja Cho , Kwang-ho Cheong , Chang-eun Yoo , Seung-yeon Yang
IPC: C12N1/06
CPC classification number: C12M47/06 , B01F11/0266 , B01F13/0059 , B01L3/502761 , B01L2300/0816 , C12N1/066 , C12N13/00
Abstract: A method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser are provided. According to the method and apparatus for a rapid disruption of cells or viruses using micro magnetic beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.
Abstract translation: 提供了使用微磁珠和激光快速破坏细胞或病毒的方法和装置。 根据使用微磁珠和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，使用激光二极管可以使装置小型化，DNA纯化步骤可以直接在 细胞或病毒的破坏以及含有DNA的溶液可以转移到随后的步骤中，随后的反应的抑制剂与细胞碎片和珠子一起用电磁铁去除。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决 裂解芯片可应用于LOC。
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