公开(公告)号：US20080072994A1

公开(公告)日：2008-03-27

申请号：US11846794

申请日：2007-08-29

申请人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

发明人： Young-rok KIM ,  Jun-hong MIN ,  Kak NAMKOONG ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Ki-woong HAN ,  Ki-eun KIM

IPC分类号： B65B1/14

CPC分类号： B01L9/527 ,  B01L3/5021 ,  B01L3/5027 ,  B01L2200/027 ,  B01L2300/0832 ,  B01L2400/0409 ,  C12M23/16

摘要： An apparatus introducing a fluid using a centrifugal force includes an introduction member including a chip receiver and a fluid introduction reservoir, the chip receiver receiving a first part of a microfluidic chip, the first part including an inlet, the fluid introduction reservoir storing a fluid to be introduced to the microfluidic chip, the fluid introduction reservoir having an exit formed to correspond to the inlet of the microfluidic chip received in the chip receiver, and a support member supporting a second part of the microfluidic chip, wherein the microfluidic chip is disposed between the introduction member and the support member, the apparatus is rotatable in a state where the introduction member is closer to a center of rotation than the microfluidic chip, and the fluid is introducible from the fluid introduction reservoir through the inlet into the microfluidic chip due to a centrifugal force generated by rotation.

摘要翻译： 使用离心力引入流体的装置包括引入构件，其包括芯片接收器和流体引入储存器，所述芯片接收器接收微流体芯片的第一部分，所述第一部分包括入口，所述流体引入储存器将流体存储到 引入到微流体芯片中，流体引入储存器具有形成为对应于接收在芯片接收器中的微流体芯片的入口的出口，以及支撑微流体芯片的第二部分的支撑构件，其中微流体芯片设置在 引导构件和支撑构件，该装置可以在导入构件比微流体芯片更靠近旋转中心的状态下旋转，并且流体可以从流体引入储存器通过入口引入到微流体芯片中，这是由于 由旋转产生的离心力。

公开(公告)号：US20110183325A1

公开(公告)日：2011-07-28

申请号：US13079926

申请日：2011-04-05

申请人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

发明人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

IPC分类号： C12Q1/68 ,  C12N13/00

CPC分类号： C12Q1/68 ,  C12M47/06 ,  C12N15/1006

摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.

摘要翻译： 本文提供了在单个芯片中破坏细胞并纯化核酸的方法和装置。 该方法包括用激光束照射芯片，其中芯片包括其上沉积有细胞裂解增强金属氧化物层和细胞结合金属氧化物层的固体支持物。

公开(公告)号：US20110177588A1

公开(公告)日：2011-07-21

申请号：US13079910

申请日：2011-04-05

申请人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

发明人： In-ho LEE ,  Jun-hong MIN ,  Young-rok KIM ,  Jae-ho YOU ,  Chang-eun YOO ,  Jeong-gun LEE ,  Ki-woong HAN

IPC分类号： C12M1/42 ,  C12M1/00

CPC分类号： C12Q1/68 ,  C12M47/06 ,  C12N15/1006

摘要： Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited.

公开(公告)号：US20070238109A1

公开(公告)日：2007-10-11

申请号：US11553657

申请日：2006-10-27

申请人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

发明人： Jun-hong MIN ,  In-ho LEE ,  Chang-eun YOO ,  Ki-woong HAN ,  Young-rok KIM

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12N15/1006 ,  C12N15/1003

摘要： Provided is a method of purifying nucleic acid, the method including: contacting a nucleic acid-containing sample and a solution containing a kosmotropic salt on a solid support having a hydrophilic functional group on its surface to bind the nucleic acid to the solid support. Since the solid support is used as it is without any surface treatment, manufacture of the apparatus is very easy, and nucleic acid can be bound to the solid support without specific additives in a wide pH range, so that the apparatus can be used for a Lab-On-a-Chip.

摘要翻译： 提供了一种纯化核酸的方法，所述方法包括：使含核酸的样品和含有可变色盐的溶液在其表面上具有亲水性官能团的固体支持物上接触，以将核酸结合到固体支持物上。 由于固体支持物原样使用而没有进行任何表面处理，所以装置的制造非常容易，并且核酸可以在宽的pH范围内结合固体支持物而没有特定的添加剂，使得该装置可以用于 实验室芯片

公开(公告)号：US20070269819A1

公开(公告)日：2007-11-22

申请号：US11620961

申请日：2007-01-08

申请人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

发明人： Young-rok KIM ,  Jun-hong MIN ,  In-ho LEE ,  Young-sun LEE ,  Chang-eun YOO ,  Ki-woong HAN

IPC分类号： C12Q1/68 ,  C12M3/00

CPC分类号： C12Q1/6806 ,  B01L3/5027 ,  B01L3/5088 ,  C12Q1/686 ,  C12Q2565/629 ,  C12Q2537/149 ,  C12Q2527/125

摘要： A method of sequentially performing concentration and amplification of nucleic acid in a single micro chamber includes: introducing a nucleic acid-containing sample and a solution including a kosmotropic salt to a micro chamber having a hydrophilic interior surface to concentrate the nucleic acid by binding the nucleic acid on the interior surface of the micro chamber; and performing a polymerase chain reaction (PCR) by adding a PCR mixture to the chamber. Since the nucleic acid is reversibly bound to the interior surface of the micro chamber, PCR yield is higher compared with a surface of aluminum oxide in which irreversible binding occurs. In addition, all processes are sequentially performed in a single micro chamber so that the number of samples, consumables, time, and labor for treatment and analysis can be reduced, detection sensitivity can be improved, and risk of sample cross contamination significantly reduced without sample loss by eliminating transporting of the sample. A complete automated system for concentration and amplification of nucleic acid is thus readily provided.

摘要翻译： 在单个微室中依次进行核酸的浓缩和扩增的方法包括：将含核酸的样品和包含可变性盐的溶液引入具有亲水性内表面的微室中，以通过结合核酸来浓缩核酸 酸在微室的内表面上; 并通过向该室加入PCR混合物进行聚合酶链式反应（PCR）。 由于核酸可逆地结合到微室的内表面，与其中发生不可逆结合的氧化铝的表面相比，PCR产率更高。 此外，所有过程都在单个微室中顺序进行，以便可以减少样品数量，消耗品，时间和处理和分析的劳动力，可以提高检测灵敏度，并且样品交叉污染的风险在没有样品时显着降低 通过消除样品的运输而损失。 因此容易提供用于浓缩和扩增核酸的完整的自动化系统。

公开(公告)号：US20080306249A1

公开(公告)日：2008-12-11

申请号：US12107498

申请日：2008-04-22

申请人： Myo-yong LEE ,  Ki-woong HAN

发明人： Myo-yong LEE ,  Ki-woong HAN

IPC分类号： C07K1/00

CPC分类号： C07K1/36 ,  C07K1/20 ,  C12Q1/6806 ,  C12Q2527/125

摘要： Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6 and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.

摘要翻译： 提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中R1，R2中的至少两个 和R3取代基是取代或未取代的C1-C6烷基，另一个取代基是氢原子或取代或未取代的C1-C6烷基，a是1〜6的整数，b是0或1，其中b是 当a不为1时为0; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。 通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。