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公开(公告)号：US06972329B2
公开(公告)日：2005-12-06
申请号：US10494885
申请日：2002-11-13
CPC分类号： C12Q1/6806 , C12Q2527/125 , C12Q2523/319
摘要： The invention provides methods for isolating and releasing genetic material from a solid medium, such as a DNA array, a filter, a card, or a multiple-well plate, using a wash solution comprising a photolytic agent, followed by subsequent exposure to a light source to release the genetic material, such as genomic DNA. The invention also includes devices and kits for practicing these methods.
摘要翻译： 本发明提供了使用包含光解剂的洗涤溶液从固体培养基（例如DNA阵列，过滤器，卡片或多孔板）中分离和释放遗传物质的方法，随后暴露于光 来源释放遗传物质，如基因组DNA。 本发明还包括用于实施这些方法的装置和套件。
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公开(公告)号：US5807527A
公开(公告)日：1998-09-15
申请号：US531597
申请日：1995-09-21
CPC分类号： C12Q1/68 , C12Q1/6806
摘要： A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.
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公开(公告)号：US5756126A
公开(公告)日：1998-05-26
申请号：US480135
申请日：1995-06-07
CPC分类号： C12Q1/68 , C12N15/1006 , C12Q1/6806 , C12Q1/6841 , C12Q1/686 , C12Q1/6879 , C12Q1/6886 , Y10S435/97
摘要： The present invention provides a dry solid medium for storage of genetic material, including RNA and DNA, in a form suitable for subsequent analysis. The invention also provides a dry solid medium including components which function in subsequent analysis of the genetic material using, for example, PCR, reverse transcriptase initiated PCR, LCR, RFLP, or genetic hybridization. The components for subsequent analysis include, for example, nucleotide sequences such as a primer and a target sequence stabilizer. The invention further provides methods for using the dry solid medium of the invention. The invention and methods thereof are particularly suited for analysis in automated systems.
摘要翻译： 本发明提供一种干燥固体培养基，用于以适于随后分析的形式储存包括RNA和DNA的遗传物质。 本发明还提供了干固体培养基，其包括在后续使用例如PCR，逆转录酶启动的PCR，LCR，RFLP或遗传杂交的遗传物质分析中起作用的组分。 用于后续分析的组分包括例如核苷酸序列，例如引物和靶序列稳定剂。 本发明还提供了使用本发明的干固体介质的方法。 本发明及其方法特别适用于自动化系统的分析。
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公开(公告)号：US07504363B2
公开(公告)日：2009-03-17
申请号：US11016291
申请日：2004-12-17
IPC分类号： C40B30/00
CPC分类号： C12N15/1017
摘要： In accordance with the present invention, there is provided a device, method and kit for detecting any genetic material containing moieties. The present invention enables identification of known or unknown virions or bacteria contained in a fluid therein, such as virions in a blood sample. It also provides for an amenable and highly automatable device for massed screenings and the discovery of sequence variants of known virions, previously undetected virions, and other genetic material containing moieties. In one aspect, the present invention provides a method for storage and analysis of a nucleic acid containing moiety in a biological sample, the method comprising providing a device comprising a plurality of layers, wherein the plurality of layers comprises a collection layer comprising a neutral porous matrix and a dry anticoagulant or liquefying agent, a filter layer comprising a dry solid medium comprising a solid matrix having a particle size limit that precludes eukaryotic cells, but not prokaryotic cells or virions, a separator layer, and an isolation layer comprising a dry solid medium comprising a neutral solid matrix having sorbed thereto a composition comprising a detergent; applying a biological sample to the collection layer; filtering remaining components of the biological sample through the collection layer to the filter layer; filtering remaining components of the biological sample through the filter layer to the separator layer; filtering remaining components of the biological sample through the separator layer to the isolation layer; retaining nucleic acid components in the isolation layer while removing a plurality of non-nucleic acid components; drying the isolation layer; providing at least one primer; and analyzing the nucleic acid components using PCR techniques, wherein at least two PCR primers are used.
摘要翻译： 根据本发明，提供了用于检测含有部分的任何遗传物质的装置，方法和试剂盒。 本发明能够鉴定其中的流体中包含的已知或未知的病毒粒子或细菌，例如血液样品中的病毒粒子。 它还提供了一种适合和高度自动化的装置，用于大规模筛选和发现已知病毒粒子的序列变体，以前未检测到的病毒粒子，以及含有部分的其他遗传物质。 在一个方面，本发明提供了用于存储和分析生物样品中含核酸部分的方法，所述方法包括提供包括多个层的装置，其中所述多个层包括收集层，所述收集层包含中性多孔 基质和干抗凝剂或液化剂，包含干固体培养基的过滤层，所述干固体培养基包含具有排除真核细胞但不包括原核细胞或病毒颗粒的粒度限制的固体基质，隔离层和包含干燥固体的分离层 包含吸收了含有洗涤剂的组合物的中性固体基质的介质; 将生物样品施加到收集层; 通过收集层将生物样品的剩余成分过滤到过滤层; 将生物样品的剩余组分通过过滤层过滤到分离层; 通过隔离层将生物样品的剩余组分过滤到隔离层; 在分离层中保留核酸组分，同时除去多个非核酸组分; 干燥隔离层; 提供至少一个引物; 并使用PCR技术分析核酸成分，其中使用至少两个PCR引物。
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公开(公告)号：US06627226B2
公开(公告)日：2003-09-30
申请号：US09805326
申请日：2001-03-13
IPC分类号： A61K914
CPC分类号： C12N15/1006 , B01L2200/14 , B01L2300/0838 , G01N2035/00564
摘要： The present invention provides for device for obtaining and storing genetic material. The device has a support and a head portion. The head portion is composed of a solid matrix for sorbing genetic material and a preserving mechanism for the protection of genetic material from degradation. Additionally, the present invention provides for a method of storing a sample of genetic material onto the swab device. In the preferred embodiment, the present invention utilizes polyester material as the solid matrix.
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公开(公告)号：US06447804B1
公开(公告)日：2002-09-10
申请号：US09393991
申请日：1999-09-10
IPC分类号： A61K914
CPC分类号： C12Q1/6806 , C12N15/1006 , C12Q1/686 , C12Q1/6879 , C12Q1/6886 , C12Q2549/101 , C12Q2547/107 , C12Q2565/518 , C12Q2543/101 , C12Q2521/301
摘要： A dry solid medium for storage of a sample includes a solid matrix of sorbing genetic material thereon and a mechanism for preserving and protecting the genetic material applied to the matrix. A method for storing the sample genetic material includes the steps of sorbing genetic material on the solid matrix and protecting the genetic material.
摘要翻译： 用于储存样品的干燥固体培养基包括其上的吸附遗传物质的固体基质和用于保存和保护施用于基质的遗传物质的机制。 用于储存样品遗传物质的方法包括在固体基质上吸附遗传物质并保护遗传物质的步骤。
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公开(公告)号：US06322983B1
公开(公告)日：2001-11-27
申请号：US09368592
申请日：1999-08-04
IPC分类号： C12Q168
CPC分类号： C12N15/1017 , Y10S435/97
摘要： A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.
摘要翻译： 一种用于储存DNA（包括血液DNA）的固体培养基，其包含固体基质，该固体基质具有防止掺入或吸收在基质上的DNA降解的化合物或组合物。 还公开了使用该固体培养基存储DNA并且用于DNA的回收或原位使用DNA的方法。
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8.发明授权Product and method for separation of a sample containing multiple sources of genetic material using a solid medium 有权使用固体培养基分离含有多种遗传物质来源的样品的产品和方法公开(公告)号：US06291179B1
公开(公告)日：2001-09-18
申请号：US09394008
申请日：1999-09-10
IPC分类号： C12Q168
CPC分类号： C12Q1/6806 , C12N15/1006
摘要： The present invention is directed to products and methods for storing, separating or analyzing a sample of genetic material. The invention is particularly suited for the separation of a selected genetic material from a sample containing genetic material from multiple sources.
摘要翻译： 本发明涉及用于存储，分离或分析遗传物质样品的产品和方法。 本发明特别适用于从多种来源的含有遗传物质的样品中分离选定的遗传物质。
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公开(公告)号：US5985327A
公开(公告)日：1999-11-16
申请号：US979978
申请日：1997-11-26
CPC分类号： C12N15/1017 , Y10S435/97
摘要： A solid medium for storage of DNA, including blood DNA, comprising a solid matrix having a compound or composition which protects against degradation of DNA incorporated into or absorbed on the matrix. Methods for storage of DNA using this solid medium, and for recovery of DNA or in situ use of DNA are also disclosed.
摘要翻译： 一种用于储存DNA（包括血液DNA）的固体培养基，其包含固体基质，该固体基质具有防止掺入或吸收在基质上的DNA降解的化合物或组合物。 还公开了使用该固体培养基存储DNA并且用于DNA的回收或原位使用DNA的方法。
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公开(公告)号：US5976572A
公开(公告)日：1999-11-02
申请号：US979833
申请日：1997-11-26
CPC分类号： C12Q1/68 , C12N15/1006 , C12Q1/6806 , C12Q1/6841 , C12Q1/686 , C12Q1/6879 , C12Q1/6886 , Y10S435/97
摘要： The present invention provides a dry solid medium for storage of genetic material, including RNA and DNA, in a form suitable for subsequent analysis. The invention also provides a dry solid medium including components which function in subsequent analysis of the genetic material using, for example, PCR, reverse transcriptase initiated PCR, LCR, RFLP, or genetic hybridization. The components for subsequent analysis include, for example, nucleotide sequences such as a primer and a target sequence stabilizer.The invention further provides methods for using the dry solid medium of the invention. The invention and methods thereof are particularly suited for analysis in automated systems.
摘要翻译： 本发明提供一种干燥固体培养基，用于以适于随后分析的形式储存包括RNA和DNA的遗传物质。 本发明还提供了干固体培养基，其包括在后续使用例如PCR，逆转录酶启动的PCR，LCR，RFLP或遗传杂交的遗传物质分析中起作用的组分。 用于后续分析的组分包括例如核苷酸序列，例如引物和靶序列稳定剂。 本发明还提供了使用本发明的干固体介质的方法。 本发明及其方法特别适用于自动化系统中的分析。
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