公开(公告)号：US08765475B2

公开(公告)日：2014-07-01

申请号：US12257301

申请日：2008-10-23

申请人： Lihua Zhou ,  Zhihong Wang ,  Yanwen Weng ,  Feng Li

发明人： Lihua Zhou ,  Zhihong Wang ,  Yanwen Weng ,  Feng Li

IPC分类号： G01N35/00 ,  G01N21/00 ,  G01N21/27 ,  G01N21/75 ,  G01N35/02

CPC分类号： G01N21/253 ,  G01N35/025 ,  G01N2021/3174 ,  G01N2035/0097

摘要： Various embodiments disclose a colorimetric absorbance measurement method and a system for performing the same. The method comprises driving a reaction tray carrying a plurality of reaction cuvettes to rotate at a speed; transferring or rotating filters on a filter wheel to a light path of a light beam, starting from a filter of a first wavelength; and sampling photoelectric data when the light beam has passed through each reaction cuvette in some embodiments. The method ensures consistency in the measurement and synchronization between the calibration and the sample test and reliability of the measurements. Various embodiments simplify data processing and reduce the complexity of the system.

摘要翻译： 各种实施方式公开了比色吸光度测定方法及其制造方法。 该方法包括驱动携带多个反应池的反应盘以一定速度旋转; 将滤光器的滤光器转移或旋转到从第一波长的滤光器开始的光束的光路; 并且在一些实施例中当光束通过每个反应比色皿时对光电数据进行取样。 该方法确保了校准和样品测试之间的测量和同步性的一致性以及测量的可靠性。 各种实施例简化了数据处理并降低了系统的复杂性。

公开(公告)号：US07382455B2

公开(公告)日：2008-06-03

申请号：US11564446

申请日：2006-11-29

申请人： Lihua Zhou ,  Jinhong Qiu

发明人： Lihua Zhou ,  Jinhong Qiu

IPC分类号： G01N21/25

CPC分类号： G01J3/51 ,  G01J3/0243 ,  G01J3/12 ,  G01J2003/1217 ,  G01N2201/0453

摘要： A spectrophotometric optical system of a microplate reader and a filter wheel thereof are disclosed. The filter wheel comprises a pivotable wheel body, at least one narrow-band filter with a relatively long central wavelength mounted to the wheel body, and at least one narrow-band filter with a relatively short central wavelength mounted to the wheel body. The narrow-band filter with a relatively long central wavelength is provided with a diaphragm on a front surface thereof The diaphragm is formed with a plurality of apertures. By the diaphragm attached to the front surface of the narrow-band filter with a relatively long central wavelength, an energy matching between light of longer wavelength and light of shorter wavelength may be achieved. Further, due to the uniformly distributed apertures in the diaphragm, an even light spot may be obtained.

摘要翻译： 公开了微板读数器及其滤光轮的分光光度学系统。 所述滤光轮包括可枢转的轮体，至少一个安装在所述车轮主体上的具有较长的中心波长的窄带过滤器以及至少一个具有相对短的中心波长的窄带过滤器，安装在所述车轮主体上。 具有相对长的中心波长的窄带滤光片在其前表面上设置有隔膜。隔膜形成有多个孔。 通过附着在具有较长中心波长的窄带滤光片的前表面的光阑，可以实现较长波长的光与较短波长的光之间的能量匹配。 此外，由于隔膜中均匀分布的孔，可以获得均匀的光点。

公开(公告)号：US20080062421A1

公开(公告)日：2008-03-13

申请号：US11564446

申请日：2006-11-29

申请人： Lihua ZHOU ,  Jinhong QIU

发明人： Lihua ZHOU ,  Jinhong QIU

IPC分类号： G01N21/25 ,  G01N21/51

CPC分类号： G01J3/51 ,  G01J3/0243 ,  G01J3/12 ,  G01J2003/1217 ,  G01N2201/0453

摘要： A spectrophotometric optical system of a microplate reader and a filter wheel thereof are disclosed. The filter wheel comprises a pivotable wheel body, at least one narrow-band filter with a relatively long central wavelength mounted to the wheel body, and at least one narrow-band filter with a relatively short central wavelength mounted to the wheel body. The narrow-band filter with a relatively long central wavelength is provided with a diaphragm on a front surface thereof The diaphragm is formed with a plurality of apertures. A ratio of a total effective area of the plurality of apertures to an effective area of the narrow-band filter with a relatively short central wavelength is equivalent to a ratio of energy amounts of shorter wavelength light passed through the narrow-band filter with a relatively short central wavelength to energy amounts of longer wavelength light passed through the narrow-band filter with a relatively long central wavelength when the diaphragm is not disposed. By the diaphragm attached to the front surface of the narrow-band filter with a relatively long central wavelength, an energy matching between light of longer wavelength and light of shorter wavelength may be achieved. Further, due to the uniformly distributed apertures in the diaphragm, an even light spot may be obtained.

摘要翻译： 公开了微板读数器及其滤光轮的分光光度学系统。 所述滤光轮包括可枢转的轮体，至少一个安装在所述车轮主体上的具有较长的中心波长的窄带过滤器以及至少一个具有相对短的中心波长的窄带过滤器，安装在所述车轮主体上。 具有相对长的中心波长的窄带滤光片在其前表面上设置有隔膜。隔膜形成有多个孔。 多个孔的总有效面积与具有相对短的中心波长的窄带滤光器的有效面积的比率等于通过窄带滤光器的较短波长光的能量比与相对 当不设置隔膜时，较长的中心波长与较长波长的光的能量通过具有较长的中心波长的窄带滤光器。 通过附着在具有较长中心波长的窄带滤光片的前表面的光阑，可以实现较长波长的光与较短波长的光之间的能量匹配。 此外，由于隔膜中均匀分布的孔，可以获得均匀的光点。

公开(公告)号：US20090104710A1

公开(公告)日：2009-04-23

申请号：US12257301

申请日：2008-10-23

申请人： Lihua ZHOU ,  Zhihong WANG ,  Yanwen WENG ,  Feng LI

发明人： Lihua ZHOU ,  Zhihong WANG ,  Yanwen WENG ,  Feng LI

IPC分类号： G01N21/17

CPC分类号： G01N21/253 ,  G01N35/025 ,  G01N2021/3174 ,  G01N2035/0097

摘要： Various embodiments disclose a colorimetric absorbance measurement method and a system for performing the same. The method comprises driving a reaction tray carrying a plurality of reaction cuvettes to rotate at a speed; transferring or rotating filters on a filter wheel to a light path of a light beam, starting from a filter of a first wavelength; and sampling photoelectric data when the light beam has passed through each reaction cuvette in some embodiments. The method ensures consistency in the measurement and synchronization between the calibration and the sample test and reliability of the measurements. Various embodiments simplify data processing and reduce the complexity of the system.

摘要翻译： 各种实施方式公开了比色吸光度测定方法及其制造方法。 该方法包括驱动携带多个反应池的反应盘以一定速度旋转; 将滤光器的滤光器转移或旋转到从第一波长的滤光器开始的光束的光路; 并且在一些实施例中当光束通过每个反应比色皿时对光电数据进行取样。 该方法确保了校准和样品测试之间的测量和同步性的一致性以及测量的可靠性。 各种实施例简化了数据处理并降低了系统的复杂性。