公开(公告)号：US20190249194A1

公开(公告)日：2019-08-15

申请号：US16397423

申请日：2019-04-29

申请人： George M. Church ,  Prashant G. Mali ,  Luhan Yang

发明人： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC分类号： C12N15/85 ,  C12N15/10 ,  C12N15/82 ,  C12N15/81 ,  C12N9/22 ,  C12N15/90 ,  C12N15/63 ,  C12N15/87 ,  C12N15/01

CPC分类号： C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

摘要： A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20110104787A1

公开(公告)日：2011-05-05

申请号：US12939505

申请日：2010-11-04

申请人： George M. Church ,  Luhan Yang

发明人： George M. Church ,  Luhan Yang

IPC分类号： C12N9/78 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10 ,  C12N5/071

CPC分类号： C12N15/63 ,  C07K2319/81 ,  C12N9/78 ,  C12Y305/04005

摘要： Novel methods and compositions for altering target nucleic acid (e.g., DNA e.g., genomic DNA) sequences are provided. Fusion proteins including one or more DNA binding domains and one or more DNA modifying domains are provided. Isolated polynucleotides encoding fusion proteins including one or more DNA binding domains and one or more DNA modifying domains are provided.

摘要翻译： 提供了用于改变靶核酸（例如DNA例如基因组DNA）序列的新方法和组合物。 提供融合蛋白，包括一个或多个DNA结合域和一个或多个DNA修饰结构域。 提供了编码包括一个或多个DNA结合结构域和一个或多个DNA修饰结构域的融合蛋白的分离的多核苷酸。