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公开(公告)号：US09090920B2
公开(公告)日：2015-07-28
申请号：US11991422
申请日：2006-09-07
CPC分类号： C12P17/04 , C12N9/0004
摘要： Microorganism selected from Gluconobacter, Gluconacetobacter, Acetobacter or Ketogulonicigenium, wherein a gene encoding a polypeptide with the activity of a repressor of L-sorbosone dehydrogenase (SNDH) and L-sorbose dehydrogenase (SDH) is disrupted. The gene has a polynucleotide selected from polynucleotides encoding a polypeptide comprising the amino acid sequence of which is represented by SEQ ID NO:2, polynucleotide comprising the nucleotide sequence according to SEQ ID NO:1, polynucleotides the complementary strand of which hybridizes under stringent conditions to a polynucleotide as defined above and which encodes a polypeptide with the activity of a repressor of SNDH and SDH. The microorganism produces at least 10% more Vitamin C and/or 2-KGA from L-sorbose compared to a microorganism wherein the repressor is intact.
摘要翻译： 选自葡糖杆菌，葡糖酸杆菌属，醋杆菌属或酮多糖属的微生物，其中编码具有L-山梨糖酮脱氢酶（SNDH）和L-山梨糖脱氢酶（SDH）的阻遏物的活性的多肽的基因被破坏。 该基因具有选自编码包含SEQ ID NO：2所示的氨基酸序列的多肽的多核苷酸的多核苷酸，包含根据SEQ ID NO：1的核苷酸序列的多核苷酸，其互补链在严格条件下杂交的多核苷酸 涉及如上定义的多核苷酸，其编码具有SNDH和SDH阻遏物活性的多肽。 与其中阻遏物是完整的微生物相比，微生物从L-山梨糖产生至少10％的维生素C和/或2-KGA。
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公开(公告)号：US08940503B2
公开(公告)日：2015-01-27
申请号：US12801629
申请日：2010-06-17
申请人： Masako Shinjoh
发明人： Masako Shinjoh
CPC分类号： C12N9/0006 , C12P1/04
摘要： The present invention relates to microorganisms genetically engineered to increase yield and/or efficiency of biomass production from a carbon source, such as e.g. glucose. Processes for generating such microorganisms are also provided by the present invention. The invention also relates to polynucleotide sequences comprising genes that encode proteins that are involved in the bioconversion of a carbon source such as e.g. glucose into biomass. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. Also included are processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms leading to a microorganism with reduced carbon source diversion, i.e. higher yield and/or efficiency of biomass production from a carbon source such as e.g. glucose.
摘要翻译： 本发明涉及基因工程改造以增加来自碳源的生物质生产的产量和/或效率的微生物，例如， 葡萄糖。 产生这种微生物的方法也由本发明提供。 本发明还涉及包含编码参与碳源的生物转化的蛋白质的基因的多核苷酸序列，例如碳源。 葡萄糖转化为生物量。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 还包括使用多核苷酸和修饰的多核苷酸序列来转化宿主微生物的过程，所述宿主微生物导致具有降低的碳源转移的微生物，即来自碳源的生物质产量的较高产量和/或效率，例如。 葡萄糖。
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公开(公告)号：US08338144B2
公开(公告)日：2012-12-25
申请号：US12701748
申请日：2010-02-08
申请人： Alan Berry , Connie Lee , Anne Françoise Mayer , Masako Shinjoh
发明人： Alan Berry , Connie Lee , Anne Françoise Mayer , Masako Shinjoh
IPC分类号： C12P17/04
CPC分类号： C12P17/04 , C12N9/0006 , C12N9/0008 , C12P7/60
摘要： The present invention discloses an isolated polynucleotide molecule derived from a polynucleotide encoding a polypeptide having L-sorbosone dehydrogenase activity comprising a partial nucleotide sequence of at least 20 consecutive nucleotides of SEQ ID NO:1. The present invention further relates to a process for the production of L-ascorbic acid in high yield, in particular a process using resting cells of a microorganism able to convert given carbon sources into vitamin C. The thus obtained vitamin C may be further processed by purification and/or separation steps.
摘要翻译： 本发明公开了衍生自编码具有L-山梨糖酮脱氢酶活性的多肽的多核苷酸的分离的多核苷酸分子，其包含SEQ ID NO：1的至少20个连续核苷酸的部分核苷酸序列。 本发明还涉及以高产率生产L-抗坏血酸的方法，特别是使用能够将给定碳源转化成维生素C的微生物的静息细胞的方法。由此获得的维生素C可以进一步由 纯化和/或分离步骤。
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公开(公告)号：US20120058563A1
公开(公告)日：2012-03-08
申请号：US13254049
申请日：2010-03-04
CPC分类号： C12P17/04 , C12N9/0006 , C12P7/60
摘要： The present invention relates to the production of recombinant microorganisms, in particular of the genus Gluconobacter, for production of 2-keto-L-gulonic acid (2-KGA) and/or L-ascorbic acid (hereinafter also referred to as Vitamin C), wherein the microorganism has been modified to overexpress L-sorbose dehydrogenase (SDH). This overexpression has been achieved by introducing of one or more copies of a polynucleotide encoding SDH into the genome of the host microorganism resulting in enhanced yield, production, and/or efficiency of 2-KGA production and/or Vitamin C compared to a non-modified microorganism. Expression of said one or more extra-copies of sdh is dependent on the integration site. The invention also relates to genetically engineered microorganisms and their use for the production of 2-KGA and/or Vitamin C.
摘要翻译： 本发明涉及用于生产2-酮-L-古洛糖酸（2-KGA）和/或L-抗坏血酸（以下也称为维生素C）的重组微生物，特别是葡萄糖酸杆菌属的生产， ，其中所述微生物已被修饰以过表达L-山梨糖脱氢酶（SDH）。 通过将一个或多个编码SDH的多核苷酸的拷贝引入宿主微生物的基因组中可以实现这一过表达，导致与非特异性抗体相比，2-KGA生产和/或维生素C的产量，生产和/或效率提高， 修饰微生物。 所述一个或多个sdh副本的表达依赖于整合位点。 本发明还涉及基因工程微生物及其用于生产2-KGA和/或维生素C的用途。
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公开(公告)号：US20090130725A1
公开(公告)日：2009-05-21
申请号：US11883639
申请日：2006-02-10
申请人： Bastian Chevreux , Anne F. Mayer , Masako Shinjoh
发明人： Bastian Chevreux , Anne F. Mayer , Masako Shinjoh
CPC分类号： C12P17/04 , C12N9/0006
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译： 本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。
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公开(公告)号：US20090017493A1
公开(公告)日：2009-01-15
申请号：US11883780
申请日：2006-02-10
申请人： Bastien Chevreux , Anne Mayer , Anja Meury , Nigel Mouncey , Masako Shinjoh
发明人： Bastien Chevreux , Anne Mayer , Anja Meury , Nigel Mouncey , Masako Shinjoh
CPC分类号： C12P17/04 , C12N9/0006
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译： 本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。
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7.发明授权Enzymatic process for the manufacture of L-ascorbic acid and D-erythorbic acid 失效用于制造L-抗坏血酸和D-异抗坏血酸的酶法公开(公告)号：US07465563B2
公开(公告)日：2008-12-16
申请号：US10494886
申请日：2002-10-31
申请人： Tatsuo Hoshino , Tatsuya Kiyasu , Masako Shinjoh
发明人： Tatsuo Hoshino , Tatsuya Kiyasu , Masako Shinjoh
IPC分类号： C12P17/04 , C07D305/12
CPC分类号： C12P17/04
摘要： A process for producing L-ascorbic acid from 2-keto-L-gulonic acid or D-erythorbic acid from 2-keto-D-gluconic acid by contacting 2-keto-L-gulonic acid or 2-keto-D-gluconic acid, respectively, with an enzym having α-amylase activity in solution. The solvent for this reaction can be water, an aqueous alcohol, an organic solvent or a mixture thereof. In each case, the starting material can be in the form of the free acid, the sodium salt, or the calcium salt.
摘要翻译： 通过使2-酮-L-古洛糖酸或2-酮-D-葡萄糖酸接触从2-酮-L-古洛糖酸或D-异抗坏血酸从2-酮-D-葡萄糖酸生产L-抗坏血酸的方法 分别具有在溶液中具有α-淀粉酶活性的酶。 该反应的溶剂可以是水，含水醇，有机溶剂或其混合物。 在每种情况下，原料可以是游离酸，钠盐或钙盐的形式。
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8.发明申请公开(公告)号：US20080305532A1
公开(公告)日：2008-12-11
申请号：US11883781
申请日：2006-02-10
CPC分类号： C12P7/60 , C07K14/195 , C12P17/04
摘要： The present invention relates to a newly identified gene that encodes a protein that is involved in the synthesis of L-ascorbic acid (hereinafter also referred to a Vitamin C). The protein is coenzyme PQQ synthesis protein B. The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译： 本发明涉及新鉴定的参与L-抗坏血酸合成的蛋白质的基因（以下也称为维生素C）。 蛋白质是辅酶PQQ合成蛋白B.本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。
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公开(公告)号：US20080176297A1
公开(公告)日：2008-07-24
申请号：US11883723
申请日：2006-02-10
CPC分类号： C12P17/04 , C07K14/195
摘要： The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C.
摘要翻译： 本发明涉及编码参与L-抗坏血酸合成（以下也称为维生素C）的蛋白质的新鉴定的基因。 本发明还涉及包含新基因及其片段的全长多核苷酸序列的多核苷酸，由多核苷酸及其片段编码的新多肽及其功能等同物。 本发明还涉及所述多核苷酸和多肽在从微生物生产维生素C中作为生物技术工具的用途，其中所述多核苷酸和/或编码多肽的修饰对产量，产量和/或 所述微生物中发酵产物的生产效率。 还包括使用多核苷酸和修饰的多核苷酸序列转化宿主微生物的方法/方法。 本发明还涉及基因工程微生物及其用于直接生产维生素C的用途。
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10.发明授权
公开(公告)号：US5399496A
公开(公告)日：1995-03-21
申请号：US935144
申请日：1992-08-24
申请人： Akio Fujiwara , Tatsuo Hoshino , Masako Shinjoh
发明人： Akio Fujiwara , Tatsuo Hoshino , Masako Shinjoh
CPC分类号： C12N9/0006 , C12N15/74 , C12P7/60
摘要： Novel shuttle vectors which are useful as shuttle vectors among microorganisms belonging to Escherichia coli, Gluconobacter and Acetobacter comprising one or more marker genes, a replication origin functional in Escherichia coli, a replication origin functional in Gluconobacter oxydans and a Mob site.
摘要翻译： 新颖的穿梭载体可用作属于大肠杆菌，葡糖杆菌和醋杆菌的微生物中的穿梭载体，其包含一个或多个标记基因，在大肠杆菌中起作用的复制起点，在氧化葡糖杆菌中起作用的复制起点和Mob位点。
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