利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

29 条记录 (耗时 0.028 秒)

    ROTAVIRUS-LIKE PARTICLE PRODUCTION IN PLANTS
    1.
    发明申请
    ROTAVIRUS-LIKE PARTICLE PRODUCTION IN PLANTS 审中-公开
    在植物中的旋风样颗粒生产

    公开(公告)号：US20150216961A1

    公开(公告)日：2015-08-06

    申请号：US14398650

    申请日：2013-05-10

    申请人： MEDICAGO INC. ,  MITSUBISHI TANABE PHARMA CORPORATION

    发明人： Marc-Andre D'Aoust ,  Nathalie Landry ,  Pierre-Olivier Lavoie ,  Masaaki Arai ,  Naomi Asahara ,  David Levi Rutendo Mutepfa ,  Inga Isabel Hitzeroth ,  Edward Peter Rybicki

    IPC分类号： A61K39/15 ,  C07K14/005 ,  C12N7/00

    CPC分类号： A61K39/15 ,  A61K39/12 ,  A61K2039/5258 ,  C07K14/005 ,  C12N7/00 ,  C12N7/02 ,  C12N15/8203 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8221 ,  C12N15/8258 ,  C12N2720/12334 ,  C12N2720/12351

    摘要： A method of producing a virus-like particle (VLP) in a plant is provided. The method comprises introducing a first nucleic acid into the plant, or portion of the plant. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein for example but not limited to rotavirus protein VP2. The nucleotide sequence may further comprise one or more than one amplification element and/or a compartment targeting sequence. A second nucleic acid might be introduced into the plant, or portion of the plant. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP6. Optionally, a third nucleic acid and/or fourth nucleic acid might be introduced into the plant, or portion of the plant. The third nucleic acid comprises a third regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP4. The fourth nucleic acid comprises a fourth regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more rotavirus structural protein, for example but not limited to rotavirus protein VP7. The plant or portion of the plant is incubated under conditions that permit the expression of the nucleic acids, thereby producing the VLP.

    摘要翻译： 提供了一种在植物中生产病毒样颗粒（VLP）的方法。 该方法包括将第一核酸引入植物或植物的一部分。 所述第一核酸包含在植物中活性的第一调节区，其可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP2）的核苷酸序列。 核苷酸序列还可以包含一个或多于一个扩增元件和/或隔室靶向序列。 可以将第二种核酸引入植物或植物的一部分。 第二核酸包含在植物中有活性的第二调控区，并可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP6）的核苷酸序列。 任选地，第三核酸和/或第四核酸可以被引入植物或植物的一部分。 第三核酸包含在植物中活性的第三调节区，并且与编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP4）的核苷酸序列可操作地连接。 第四核酸包含在植物中活性的第四调节区，并且可操作地连接于编码一种或多种轮状病毒结构蛋白（例如但不限于轮状病毒蛋白VP7）的核苷酸序列。 将植物或植物的一部分在允许核酸表达的条件下温育，从而产生VLP。

    Rotavirus-like particle production in plants
    2.
    发明授权
    Rotavirus-like particle production in plants 有权

    公开(公告)号：US10287555B2

    公开(公告)日：2019-05-14

    申请号：US15545362

    申请日：2016-01-21

    申请人： MEDICAGO, INC. ,  MITSUBISHI TANABE PHARMA CORPORATION

    发明人： Pierre-Olivier Lavoie ,  Marc-Andre D'Aoust

    IPC分类号： C12N7/04 ,  A61K39/12 ,  C12N7/02 ,  C12N15/82 ,  C12N15/86 ,  A61K39/00

    摘要： A method of producing a rotavirus-like particle (RLP) in a plant is provided. The method comprises expressing within a host or host cell for example a plant, portion of a plant or plant cell one or more nucleic acid comprising one or more regulatory region operatively linked to a first, second and third nucleotide sequence, the regulatory region active in the host or host cell. The first nucleotide sequence encoding a first rotavirus protein, the second nucleotide sequence encoding a second rotavirus protein and the third nucleotide sequence encoding a third rotavirus protein. The first, second and third encode rotavirus protein NSP4 and VP2 or VP6 and VP4 or VP7. The host or host cell is incubated under conditions that permit the expression of the nucleic acids, so that NSP4 and either VP2 of VP6 and VP4 or VP7 are expressed, thereby producing the RLP. Hosts comprising the RLP, compositions comprising the RLP and method for using the composition are also provided.

    Jasmonic acid pathway activator
    7.
    发明授权
    Jasmonic acid pathway activator 有权

    公开(公告)号：US10907168B2

    公开(公告)日：2021-02-02

    申请号：US15740625

    申请日：2016-06-30

    申请人： MEDICAGO INC. ,  UNIVERSITE LAVAL

    发明人： Marc-Andre D'Aoust ,  Stephanie Robert ,  Marie-Claire Goulet ,  Dominique Michaud ,  Frank Sainsbury

    IPC分类号： C12N15/82

    摘要： A method of increasing expression of an heterologous protein of interest in a plant or portion of the plant is provided. The method comprises treating the plant or portion of the plant with a jasmonate-pathway activator, and introducing a nucleotide sequence operably linked to a regulatory region derived from a DNA plant virus and encoding the heterologous protein of interest into the plant or portion of the plant. Alternatively, the plant or plant portion may comprise the nucleic acid and encoding the heterologous protein of interest, and the plant or portion of the plant is treated with the jasmonate pathway activator. The treated plant is incubated under conditions to permit expression of the nucleotide sequence encoding the heterologous protein of interest.

    Picornavirus-like particle production in plants
    10.
    发明授权
    Picornavirus-like particle production in plants 有权

    公开(公告)号：US10590173B2

    公开(公告)日：2020-03-17

    申请号：US16228091

    申请日：2018-12-20

    申请人： MEDICAGO INC.

    发明人： Marc-Andre D'Aoust ,  Pierre-Olivier Lavoie ,  Manon Couture ,  Lucie Poulin ,  Louis-Philippe Vezina

    IPC分类号： C12N7/00 ,  C07K14/005 ,  C07K16/10 ,  C12N15/82 ,  A61K39/125 ,  A61K39/13

    摘要： A method of producing a picornavirus-like particle (PVLP) in a plant is provided. The method comprises introducing a first nucleic acid and a second nucleic acid into the plant, portion of the plant, or a plant cell. The first nucleic acid comprising a first regulatory region active in the plant operatively linked to a nucleotide sequence encoding a polyprotein. The second nucleic acid comprises a second regulatory region active in the plant and operatively linked to a nucleotide sequence encoding one or more protease. The plant, portion of the plant, or plant cell is incubated under conditions that permit the expression of the nucleic acids, thereby producing the PVLP. A PVLP comprising the polyprotein is also provided.