公开(公告)号：US20050048561A1

公开(公告)日：2005-03-03

申请号：US10960224

申请日：2004-10-06

申请人： Mack Fulwyler ,  Joe Gray

发明人： Mack Fulwyler ,  Joe Gray

IPC分类号： G01N33/558 ,  C12Q1/68 ,  C12M1/34 ,  G01N33/53

CPC分类号： B01L3/502784 ,  B01J19/0046 ,  B01J2219/00479 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00657 ,  B01J2219/00722 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N33/558 ,  Y10S436/807 ,  Y10T436/117497

摘要： The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention. A preferred device includes: a substrate with a channel formed in its surface, and a cover element that overlies and seals the channel. Binding partner(s) are affixed to the surface of the cover element facing the channel lumen.

摘要翻译： 本发明提供用于检测样品中存在一种或多种目标分析物的方法和装置，其中使用其中固定有每个目标分析物的一个或多个结合配偶体的通道。 通过将样品通过通道输送到每个连续的结合配偶体进行测定，使得存在于所述样品中的目标分析物与相应的结合配偶体结合。 然后将样品转移超过结合配偶体，随后检测与每个结合配偶体结合的任何目标分析物。 在一个实施方案中，通过使用分段运输来增加结合效率，其中在运输期间与样品不混溶的流体的第一推注或气泡先于样品，并且与样品不混溶的流体的第二推注或气泡 遵循样本。 许多配置对于本发明的设备是可能的。 优选的装置包括：具有在其表面中形成的通道的基底和覆盖并密封通道的盖元件。 绑定伙伴被固定在覆盖元件面向通道腔的表面上。

公开(公告)号：US20060160162A1

公开(公告)日：2006-07-20

申请号：US11293047

申请日：2005-12-01

申请人： Mack Fulwyler ,  Joe Gray

发明人： Mack Fulwyler ,  Joe Gray

IPC分类号： G01N33/537 ,  G01N33/53 ,  G01N33/543

CPC分类号： B01L3/502784 ,  B01J19/0046 ,  B01J2219/00479 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00657 ,  B01J2219/00722 ,  B01L2200/0673 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0487 ,  G01N33/558 ,  Y10S436/807 ,  Y10T436/117497

摘要： The invention provides methods and devices for detecting the presence of one or more target analytes in a sample employing a channel having affixed therein one or more binding partners for each target analyte. Assays are carried out by transporting the sample through the channel to each successive binding partner so that target analyte present in said sample binds to the corresponding binding partner. The sample is then transported beyond the binding partner(s), followed by detection of any target analyte bound to each binding partner. In one embodiment, binding efficiency is increased by the use of segmented transport, wherein a first bolus or bubble of a fluid that is immiscible with the sample precedes the sample during transport and a second bolus or bubble of a fluid that is immiscible with the sample follows the sample. Many configurations are possible for the device of the invention. A preferred device includes: a substrate with a channel formed in its surface, and a cover element that overlies and seals the channel. Binding partner(s) are affixed to the surface of the cover element facing the channel lumen.