公开(公告)号：US20190040458A1

公开(公告)日：2019-02-07

申请号：US15929030

申请日：2018-06-29

申请人： Intel Corporation

发明人： Xing Su ,  Grace Credo

IPC分类号： C12Q1/6844 ,  B01J19/00 ,  B01L3/00

CPC分类号： C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00387 ,  B01J2219/00511 ,  B01J2219/00596 ,  B01J2219/00653 ,  B01J2219/00689 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01L3/502753 ,  B01L3/502784 ,  B01L2200/025 ,  C07H21/04

摘要： DNA synthesis devices, systems, and methods are disclosed. An apparatus can include a synthesizer chip having an array of reaction units in a predetermined pattern, each reaction unit including a reaction surface and a reaction electrode of an IC array of reaction electrodes, and a synthesizer chip controller coupled to the IC array of reaction electrodes configured to address each reaction electrode individually. The apparatus can also include a reagent delivery chip positionable above the synthesizer chip, comprising an array of reagent delivery units arranged in the predetermined pattern, each reagent delivery unit including a reagent electrode of an IC array of reagent electrodes and each reagent delivery unit configured to receive and deliver a droplet of reagent fluid having a volume of 1 picoliter or less, and a reagent delivery chip controller coupled to the IC array of reagent electrodes configured to address each reagent electrode individually.

公开(公告)号：US20170326525A1

公开(公告)日：2017-11-16

申请号：US15667671

申请日：2017-08-03

申请人： Chevron Phillips Chemical Company LP

发明人： Eric J. Netemeyer ,  Michael S. Matson ,  Greg L. Thomas ,  Dale M. Solaas ,  Christopher R. Tully ,  Joe E. Figard

IPC分类号： B01J19/24 ,  B01J19/12 ,  C07C319/04 ,  B01J19/00 ,  C07C319/18

CPC分类号： B01J19/2415 ,  B01J19/006 ,  B01J19/0073 ,  B01J19/12 ,  B01J19/123 ,  B01J19/2405 ,  B01J2219/00162 ,  B01J2219/00511 ,  B01J2219/0883 ,  B01J2219/0884 ,  B01J2219/0888 ,  B01J2219/185 ,  C07C319/02 ,  C07C319/04 ,  C07C319/16 ,  C07C319/18 ,  C07C321/04 ,  C07C321/14

摘要： The present invention discloses high pressure flow reactor vessels and associated systems. Also disclosed are processes for producing thiol compounds and sulfide compounds utilizing these flow reactor vessels.

公开(公告)号：US09695472B2

公开(公告)日：2017-07-04

申请号：US12950729

申请日：2010-11-19

申请人： Xing Su ,  Kai Wu

发明人： Xing Su ,  Kai Wu

IPC分类号： C12Q1/68 ,  B82Y30/00 ,  B01L3/00 ,  G01N27/414

CPC分类号： C12Q1/6874 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00641 ,  B01J2219/00722 ,  B01L3/5085 ,  B82Y30/00 ,  C12Q1/6825 ,  G01N27/414 ,  C12Q2565/301 ,  C12Q2533/101 ,  C12Q2521/101 ,  C12Q2565/607 ,  C12Q2525/125 ,  C12Q2521/319

摘要： Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction regions containing electronic sensors to monitor changes in solutions contained in the reaction regions. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.

公开(公告)号：US20170147748A1

公开(公告)日：2017-05-25

申请号：US15426860

申请日：2017-02-07

申请人： Synthetic Genomics, Inc.

发明人： Peer F. Staehler ,  Cord F. Staehler ,  Manfred Mueller

IPC分类号： G06F19/22 ,  G06F19/28 ,  C12P19/34

CPC分类号： G16B30/00 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00317 ,  B01J2219/00432 ,  B01J2219/00436 ,  B01J2219/00439 ,  B01J2219/00441 ,  B01J2219/00448 ,  B01J2219/00479 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00529 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00603 ,  B01J2219/00605 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00702 ,  B01J2219/00704 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502707 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/069 ,  B01L2300/0864 ,  B82Y30/00 ,  C12N15/10 ,  C12N15/66 ,  C12P19/34 ,  C40B40/06 ,  C40B40/10 ,  C40B50/14 ,  G03F7/70216 ,  G16B50/00

摘要： The invention relates to a method for producing polymers, in particular synthetic nucleic acid double strands of optional sequence, comprising the steps: (a) provision of a support having a surface area which contains a plurality of individual reaction areas, (b) location-resolved synthesis of nucleic acid fragments having in each case different base sequences in several of the individual reaction areas, and (c) detachment of the nucleic acid fragments from individual reaction areas.

公开(公告)号：US20160332133A1

公开(公告)日：2016-11-17

申请号：US15131101

申请日：2016-04-18

申请人： LIFE TECHNOLOGIES CORPORATION

发明人： Robert HESS ,  John LINTON ,  Tanya S. KANIGAN ,  Colin BRENAN ,  Can OZBAL

IPC分类号： B01J19/00 ,  B01L3/02 ,  G01N30/46 ,  C40B60/14 ,  G01N30/60 ,  B01L3/00 ,  C12M1/32

CPC分类号： B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00319 ,  B01J2219/00351 ,  B01J2219/0036 ,  B01J2219/00369 ,  B01J2219/00387 ,  B01J2219/00389 ,  B01J2219/00423 ,  B01J2219/0043 ,  B01J2219/00479 ,  B01J2219/00495 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00673 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  B01L3/5025 ,  B01L3/50255 ,  B01L3/5085 ,  B01L3/50857 ,  B01L99/00 ,  B01L2200/0657 ,  B01L2300/0845 ,  C12M23/12 ,  C40B60/14 ,  G01N30/466 ,  G01N30/6091 ,  G01N30/6095 ,  G01N30/82 ,  G01N2030/8417

摘要： The invention features methods of making devices, or “platens”, having a high-density array of through-holes, as well as methods of cleaning and refurbishing the surfaces of the platens. The invention further features methods of making high-density arrays of chemical, biochemical, and biological compounds, having many advantages over conventional, lower-density arrays. The invention includes methods by which many physical, chemical or biological transformations can be implemented in serial or in parallel within each addressable through-hole of the devices. Additionally, the invention includes methods of analyzing the contents of the array, including assaying of physical properties of the samples.

摘要翻译： 本发明的特征在于制造具有高密度阵列通孔的装置或“压板”的方法，以及清洁和翻新压板表面的方法。 本发明还涉及制备化学，生物化学和生物化合物的高密度阵列的方法，其具有优于常规的较低密度阵列的许多优点。 本发明包括许多物理，化学或生物转化可以在装置的每个可寻址通孔内串联或并行地实现的方法。 此外，本发明包括分析阵列的内容物的方法，包括测定样品的物理性质。

公开(公告)号：US09040237B2

公开(公告)日：2015-05-26

申请号：US11226696

申请日：2005-09-13

申请人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Zhang Jingwu ,  Mineo Yamakawa ,  Val M. Dubin

发明人： Tae-Woong Koo ,  Selena Chan ,  Xing Su ,  Zhang Jingwu ,  Mineo Yamakawa ,  Val M. Dubin

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12M3/00 ,  C12M1/34 ,  B82Y30/00 ,  B01L3/00 ,  G01N27/414

CPC分类号： C12Q1/6874 ,  B01J2219/00317 ,  B01J2219/00511 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00641 ,  B01J2219/00722 ,  B01L3/5085 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148

摘要： Embodiments of the present invention provide devices methods for sequencing DNA using arrays of reaction cavities containing sensors to monitor changes in solutions contained in the reaction cavities. Additional embodiments provide devices and methods for sequencing DNA using arrays of reaction cavities that allow for optical monitoring of solutions in the reaction cavities. Test and fill reaction schemes are disclosed that allow DNA to be sequenced. By sequencing DNA using parallel reactions contained in large arrays, DNA can be rapidly sequenced.

摘要翻译： 本发明的实施方案提供了使用包含传感器的反应腔阵列来测序DNA的装置方法，以监测包含在反应腔中的溶液的变化。 另外的实施方案提供了使用允许对反应腔中的溶液进行光学监测的反应腔阵列对DNA进行测序的装置和方法。 公开了允许DNA测序的测试和填充反应方案。 通过使用大阵列中包含的平行反应测序DNA，可以快速测序DNA。

公开(公告)号：US20150073061A1

公开(公告)日：2015-03-12

申请号：US14486290

申请日：2014-09-15

申请人： Cambridge Enterprise Limited

发明人： Wolfgang Andreas BAUER ,  Wilhelm T.S. HUCK ,  Martin FISCHLECHNER

IPC分类号： B01L3/00 ,  B01F17/00

CPC分类号： B01L3/502707 ,  B01F17/00 ,  B01J2219/00511 ,  B01J2219/0059 ,  B01J2219/00637 ,  B01J2219/00783 ,  B01J2219/00788 ,  B01J2219/00831 ,  B01J2219/00833 ,  B01J2219/00837 ,  B01J2219/0097 ,  B01L2200/0636 ,  B01L2300/0867 ,  B01L2300/16 ,  B01L2300/161 ,  B05C7/04 ,  B05D7/22 ,  B05D7/222 ,  B81B2201/0214 ,  B81B2201/05 ,  B81B2201/052 ,  B81B2201/058 ,  B81C1/00119 ,  B81C1/00206 ,  Y10T137/8593

摘要： We describe a method of layer-by-layer deposition of a plurality of layers of material onto the wall or walls of a channel of a microfluidic device, the method comprising: loading a tube with a series of segments of solution, a said segment of solution bearing a material to be deposited; coupling said tube to said microfluidic device; and injecting said segments of solution into said microfluidic device such that said segments of solution pass, in turn, through said channel depositing successive layers of material to perform said layer-by-layer deposition onto said wall or walls of said channel. Embodiments of the methods are particularly useful for automated surface modification of plastic, for example PDMS (Poly(dimethylsiloxane)), microchannels. We also describe methods and apparatus for forming double-emulsions.

摘要翻译： 我们描述了将多层材料层逐层沉积到微流体装置的通道的壁或壁上的方法，该方法包括：将具有一系列溶液段的管装载， 含有待沉积材料的溶液; 将所述管连接到所述微流体装置; 以及将所述溶液段注射到所述微流体装置中，使得所述溶液段又通过所述通道沉积连续的材料层，以在所述通道的所述壁或壁上执行所述逐层沉积。 该方法的实施方案对于塑料的自动表面改性特别有用，例如PDMS（聚（二甲基硅氧烷）），微通道。 我们还描述了形成双重乳液的方法和设备。

公开(公告)号：US20140377852A1

公开(公告)日：2014-12-25

申请号：US14479290

申请日：2014-09-06

申请人： CyVek, Inc.

发明人： Martin A. Putnam ,  Jeffrey T. Branciforte ,  Charles O. Stanwood

IPC分类号： G01N35/00

CPC分类号： B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00511 ,  B01J2219/00522 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00596 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502738 ,  B01L2200/021 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0631 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0838 ,  B01L2300/0864 ,  B01L2300/087 ,  B01L2300/0874 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/168 ,  B01L2300/1805 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0638 ,  B01L2400/0655 ,  B01L2400/086 ,  G01N21/05 ,  G01N21/645 ,  G01N21/6458 ,  G01N33/54366 ,  G01N35/00029 ,  G01N2021/0346 ,  G01N2021/058 ,  G01N2035/00039 ,  G01N2035/00108 ,  G01N2035/00158 ,  G01N2035/00752 ,  Y10T29/494

摘要： An operating and reading instrument for performing an assay employing a portable microfluidic assay cartridge, the instrument comprising a translatable table under automated control, the translatable table carrying a receiving region for the portable cartridge and carrying a port system connectible to the cartridge that includes at least one remotely automated valve carried by the translatable table, the valve arranged to apply pressurized flowable substance at selected times to the cartridge while the cartridge is on the translatable table.

摘要翻译： 一种用于使用便携式微流控测定盒执行测定的操作和阅读仪器，所述仪器包括在自动控制下的可翻译工作台，所述可移动工作台承载用于所述便携式存储器的接收区域，并且携带可连接到所述盒的端口系统，所述端口系统至少包括 一个远程自动化的阀，由可移动工作台承载，该阀设置成在该盒位于可平移台上时，将选定的时间加压的可流动物质施加到该盒。

公开(公告)号：US08875981B2

公开(公告)日：2014-11-04

申请号：US13490717

申请日：2012-06-07

申请人： Brian Neltner ,  David M. King ,  Rick Bryan Woodruff

发明人： Brian Neltner ,  David M. King ,  Rick Bryan Woodruff

IPC分类号： B01J8/06 ,  B01J19/24 ,  B23K37/00 ,  B23K31/02 ,  B23K1/00 ,  B01J19/00

CPC分类号： B23K1/0008 ,  B01J19/0046 ,  B01J19/0093 ,  B01J2219/00286 ,  B01J2219/00495 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00747 ,  B01J2219/00795 ,  B01J2219/00822 ,  B01J2219/00835 ,  B01J2219/00842 ,  B01J2219/00869 ,  B01J2219/00873 ,  B01J2219/00876 ,  B23K2101/36

摘要： A cartridge microreactor includes a tubular heater, a metal capillary reaction tubing and a brazing alloy. The tubular heater includes an inner tube surrounded by an outer heater sheath. The metal capillary reaction tubing has a high aspect ratio, an inlet port and an outlet port and is wrapped around the tubular heater so that it is in intimate thermal contact with the heater sheath. The brazing alloy permanently bonds the tubular heater with the metal capillary reaction tubing. The metal capillary reaction tubing is configured to be heated by the tubular heater and is configured to receive one or more reactants through the inlet port and to allow the reactants to react under a selected temperature and pressure and to produce one or more products exiting through the outlet port.

摘要翻译： 墨盒微反应器包括管状加热器，金属毛细管反应管和钎焊合金。 管状加热器包括由外部加热器护套包围的内管。 金属毛细管反应管具有高纵横比，入口和出口，并且缠绕在管状加热器周围，使得其与加热器护套紧密地热接触。 钎焊合金将管状加热器与金属毛细管反应管永久地接合。 金属毛细管反应管被配置为被管状加热器加热，并且被配置为通过入口接收一种或多种反应物并允许反应物在选定的温度和压力下反应并产生一个或多个通过 出口口

公开(公告)号：US08821816B2

公开(公告)日：2014-09-02

申请号：US12123378

申请日：2008-05-19

申请人： Daniel P. Little ,  Maryanne J. O'Donnell-Maloney ,  Charles R. Cantor ,  Hubert Köster

发明人： Daniel P. Little ,  Maryanne J. O'Donnell-Maloney ,  Charles R. Cantor ,  Hubert Köster

IPC分类号： G01N37/00 ,  H01J49/04 ,  B01L3/02

CPC分类号： H01J49/0418 ,  B01J19/0046 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00378 ,  B01J2219/00387 ,  B01J2219/00468 ,  B01J2219/00497 ,  B01J2219/005 ,  B01J2219/00504 ,  B01J2219/00511 ,  B01J2219/0052 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/0244 ,  B01L3/0262 ,  B01L3/0268 ,  C07B2200/11 ,  C07F9/2408 ,  C07F9/2429 ,  C07H21/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/14 ,  G01N35/1067 ,  G01N35/1074 ,  G01N2035/1037 ,  G01N2035/1069 ,  Y10T428/26 ,  Y10T428/31678 ,  Y10T428/31855 ,  Y10T436/119163 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/2575

摘要： Provided herein are substrates for matrix-assisted laser-desorption ionization (MALDI) mass spectrometric analysis. Each spot includes 3-hydroxypicolinic acid matrix and no analyte.

摘要翻译： 本文提供了用于基质辅助激光解吸电离（MALDI）质谱分析的底物。 每个斑点包括3-羟基吡啶甲酸基质，无分析物。