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    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
    3.
    发明授权
    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method 失效

    公开(公告)号：US5814465A

    公开(公告)日：1998-09-29

    申请号：US460934

    申请日：1995-06-05

    申请人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    发明人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    IPC分类号： C12N15/09 ,  C07K19/00 ,  C12N1/21 ,  C12N9/02 ,  C12Q1/26 ,  C12Q1/66 ,  C12R1/185 ,  C12R1/19 ,  C12N9/96 ,  G01N33/566

    CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C07K2319/00

    摘要： The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor by the use of said biotinated firefly luciferase. According to the present invention, there are provided biotinated firefly luciferase, a biotinated firefly luciferase gene, a recombinant DNA, a process for producing biotinated firefly luciferase and a bioluminescent analysis method. The present invention enables the efficient production of biotinated firefly luciferase of uniform properties with the uniform structure of firefly luciferase in which one biotin molecule has been bound to a specific residue and whose activity is hardly lost by biotination and not lost even binding to streptoavidin or avidin, and the biotinated firefly luciferase of constant properties obtained in the present invention permits highly sensitive measurements in bioluminescent analysis as compared with the conventional chemically modified biotinated firefly luciferase so that the present invention is industrially extremely useful.

    Purified cyclodextrinase
    6.
    发明授权
    Purified cyclodextrinase 失效
    纯化CYCLODEXTRINASE

    公开(公告)号：US5110734A

    公开(公告)日：1992-05-05

    申请号：US533856

    申请日：1990-06-06

    申请人： Tetsuya Oguma ,  Mamoru Kikuchi ,  Kiyoshi Mizusawa ,  Shoichi Tokutake ,  Nobuyuki Yamaji

    发明人： Tetsuya Oguma ,  Mamoru Kikuchi ,  Kiyoshi Mizusawa ,  Shoichi Tokutake ,  Nobuyuki Yamaji

    IPC分类号： C12N9/24 ,  C12P19/14

    CPC分类号： C12Y302/01054 ,  C12N9/2402 ,  C12P19/14

    摘要： The present invention relates to a novel cyclodextrinase having the specific physicochemical characteristics relating to action, substrate specificities, optimum pH, stable pH, inhibition, activation and molecular, weight. This novel enzyme can be produced by culturing a specified microorganism belonging to the genus Bacillus. The invention also provides a process for preparing maltooligosaccharides by acting the present enzyme to cyclodextrins.

    摘要翻译： 本发明涉及具有与作用，底物特异性，最佳pH，稳定pH，抑制，活化和分子重量有关的特定物理化学特性的新型环糊精酶。 这种新型酶可以通过培养属于芽孢杆菌属的特定微生物来产生。 本发明还提供了通过将本发明酶作用于环糊精来制备麦芽低聚糖的方法。

    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method
    7.
    发明授权
    Biotinated firefly luciferase, a gene for biotinated firefly luciferase, a recombinant DNA, a process for producing biotinated luciferase and a bioluminescent analysis method 失效

    公开(公告)号：US5843746A

    公开(公告)日：1998-12-01

    申请号：US782118

    申请日：1997-01-13

    申请人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    发明人： Hiroki Tatsumi ,  Satoshi Fukuda ,  Mamoru Kikuchi ,  Yasuji Koyama

    IPC分类号： C12N15/09 ,  C07K19/00 ,  C12N1/21 ,  C12N9/02 ,  C12Q1/26 ,  C12Q1/66 ,  C12R1/185 ,  C12R1/19 ,  C12N15/62 ,  C12N15/53 ,  C12N15/63

    CPC分类号： C12N9/0069 ,  C12Q1/66 ,  C07K2319/00

    摘要： The present invention relates to biotinated firefly luciferase comprising a biotinated peptide and firefly luciferase linked therein, biotinated firefly luciferase having a specific amino acid sequence, a biotinated firefly luciferase gene comprising a gene coding for a biotinated peptide and a firefly luciferase gene linked therein, a biotinated firefly luciferase gene comprising a biotinated peptide gene coding for a specific amino acid sequence and a firefly luciferase gene linked therein, a recombinant DNA comprising said biotinated firefly luciferase gene inserted into a vector DNA, a process for producing biotinated firefly luciferase comprising culturing a microorganism belonging to the genus Escherichia carrying said recombinant DNA and then recovering the resulting biotinated firefly luciferase from the culture, a bioluminescent analysis method comprising using said biotinated firefly luciferase, and a bioluminescent analysis method comprising quantifying a ligand by measuring a biotinated receptor by the use of said biotinated firefly luciferase. According to the present invention, there are provided biotinated firefly luciferase, a biotinated firefly luciferase gene, a recombinant DNA, a process for producing biotinated firefly luciferase and a bioluminescent analysis method. The present invention enables the efficient production of biotinated firefly luciferase of uniform properties with the uniform structure of firefly luciferase in which one biotin molecule has been bound to a specific residue and whose activity is hardly lost by biotination and not lost even binding to streptoavidin or avidin, and the biotinated firefly luciferase of constant properties obtained in the present invention permits highly sensitive measurements in bioluminescent analysis as compared with the conventional chemically modified biotinated firefly luciferase so that the present invention is industrially extremely useful.

    Pharmaceutical preparation for prevention and/or treatment for cataract
    8.
    发明授权
    Pharmaceutical preparation for prevention and/or treatment for cataract 失效
    用于白内障预防和/或治疗的药物制剂

    公开(公告)号：US5698585A

    公开(公告)日：1997-12-16

    申请号：US631424

    申请日：1996-04-12

    申请人： Jun Yamakoshi ,  Tsutomu Sasaki ,  Hiroharu Ishikawa ,  Yukihiko Iwai ,  Takanao Matsudo ,  Kenji Mori ,  Mamoru Kikuchi

    发明人： Jun Yamakoshi ,  Tsutomu Sasaki ,  Hiroharu Ishikawa ,  Yukihiko Iwai ,  Takanao Matsudo ,  Kenji Mori ,  Mamoru Kikuchi

    IPC分类号： A61K31/341 ,  A61K31/34

    CPC分类号： A61K31/341 ,  Y10S514/912

    摘要： In order to prevent or treat cataract, present invention features 3(2H)-furanone derivatives having 3(2H)-furanone skeleton as a main structure and/or pharmaceutically acceptable salts thereof, pharmaceutical preparations containing the derivatives and/or the salts thereof, treatment methods of cataract, and their use. The 3(2H)-furanone derivatives have excellent effects for the prevention and/or treatment of cataract, for instance, senile cataract caused by oxidation disorder. Particularly, the pharmaceutical preparations have good effect for the prevention and/or treatment of cataract when they are administrated as ophtalmics.

    摘要翻译： 为了防止或治疗白内障，本发明的特征在于具有3（2H） - 呋喃酮骨架的3（2H） - 呋喃酮衍生物作为主要结构和/或其药学上可接受的盐，其含有衍生物和/或其盐的药物制剂， 白内障治疗方法及其应用。 3（2H） - 呋喃酮衍生物对于预防和/或治疗白内障具有优异的效果，例如由氧化障碍引起的老年性白内障。 特别地，药物制剂对于预防和/或治疗白内障时具有良好的效果，当它们作为眼底液给药时。