公开(公告)号：US5550058A

公开(公告)日：1996-08-27

申请号：US990718

申请日：1992-12-15

Applicant: Mark A. Corio ,  James F. Leary

Inventor： Mark A. Corio ,  James F. Leary

IPC: B07C5/00 ,  G01N15/10 ,  G01N15/14 ,  G01N33/49 ,  B07C5/04 ,  B07C5/342 ,  G01N15/00

CPC classification number: G01N15/1429 ,  G01N15/1427 ,  G01N15/1459 ,  G01N2015/1488 ,  G01N2015/149 ,  Y10S209/906

Abstract: This invention relates to a method and apparatus for flexibly controlling sorting decisions for a flow cytometer or similar instrument at a purity vs. yield ratio of sorted particles, particularly at high event rates. The system monitors distances between sequential objects to determine if they are in close enough proximity to be within a single resolution unit of the sorting device. The "friend" or "foe" status of neighboring events is considered by this system in dealing with detected "coincident" events. The system operates on information of the desirability to sort the object, i.e. droplet, based upon the properties of the individual object. A storage and retrieval method is provided for making the information available at the time a sort operation based on a sorting logic condition is performed. Accordingly, an easily reconfigurable mode of operation control is provided to allow customized sorting strategies, based on the needs of individual applications or experiments. This system may be included as an integral part of a flow cytometer/cell sorter or similar instrument and may also be provided as an outboard module to such systems.

Abstract translation: 本发明涉及一种用于灵活地控制流式细胞仪或类似仪器的分选决定的方法和装置，其分选颗粒的纯度与产率之比，特别是在高事件率下。 系统监视顺序对象之间的距离，以确定它们是否处于足够接近的位置，处于分类设备的单个分辨率单位内。 该系统在处理检测到的“重合”事件时考虑了邻居事件的“朋友”或“敌方”状态。 该系统基于对单个对象的属性对物体（即液滴）进行分类的可取性的信息进行操作。 提供了一种存储和检索方法，用于在执行基于排序逻辑条件的分类操作时使信息可用。 因此，提供了一种易于重新配置的操作控制模式，以根据个别应用或实验的需要来允许定制的排序策略。 该系统可以作为流式细胞仪/细胞分选器或类似仪器的组成部分包括在内，也可以作为外部模块提供给这样的系统。

公开(公告)号：US20120222600A1

公开(公告)日：2012-09-06

申请号：US13041261

申请日：2011-03-04

Applicant: Mark A. Corio

Inventor： Mark A. Corio

IPC: D05B49/00 ,  D05B87/00

CPC classification number: D05B1/02 ,  D05B85/10 ,  D05C11/16

Abstract: A sewing device includes a material wrapped around a spool (the spool rotating around an axis), and a needle with a hole through which the material passes. A material feeding mechanism moves the needle and feeds the material in a feeding direction, thereby unraveling the material from the spool and stitching the material into a workpiece. A controller controls the material feeding mechanism to move the needle to a first rest position above a top surface of the workpiece. The first rest position is determined by the following formula: PR1=PW1+AT; wherein PR1 is the first rest position; PW1 is a first position of the top surface of the workpiece; and AT is an amount of material to be used in forming a second stitch in the workpiece after forming a first stitch in the workpiece.

Abstract translation: 缝纫装置包括缠绕在卷轴（卷轴绕轴旋转的）的材料，以及具有孔的针，材料通过该针。 材料供给机构使针移动并沿进给方向进给材料，从而将材料从卷轴解开并将材料缝合到工件中。 控制器控制材料馈送机构将针移动到工件顶表面上方的第一静止位置。 第一个休息位置由以下公式确定：PR1 = PW1 + AT; 其中PR1是第一静止位置; PW1是工件顶面的第一位置; AT是在工件中形成第一线圈之后在工件中形成第二线圈的材料量。

公开(公告)号：US06175254B1

公开(公告)日：2001-01-16

申请号：US09240750

申请日：1999-01-29

Applicant: Mark A. Corio

Inventor： Mark A. Corio

IPC: H03K5159

CPC classification number: H03K5/007 ,  H03F3/45982 ,  H03K5/082

Abstract: An electronic circuit provides automatic compensation with reduced errors of undesirable offsets in analog and digital signals, including offsets in the input signal and offsets due to tolerances in components comprising said electronic circuit. The electronic circuit includes a minimum-hold circuit or, depending on the polarity of the signal of interest, a maximum-hold circuit to continuously determine the at-rest, or reference level of the input signal. The accuracy of compensation is therefore not a function of the duty cycle or amplitude of the input signal of interest.

Abstract translation: 电子电路提供自动补偿，减少模拟和数字信号中不期望的偏移的误差，包括输入信号中的偏移和由于包括所述电子电路的部件中的公差引起的偏移。 电子电路包括最小保持电路，或者根据感兴趣的信号的极性，具有连续地确定输入信号的静止或参考电平的最大保持电路。 因此，补偿的精度不是所关心的输入信号的占空比或幅度的函数。

公开(公告)号：US5998212A

公开(公告)日：1999-12-07

申请号：US697296

申请日：1996-08-26

Applicant: Mark A. Corio ,  James F. Leary

Inventor： Mark A. Corio ,  James F. Leary

IPC: B07C5/00 ,  G01N15/10 ,  G01N15/14 ,  G01N33/49 ,  B07C5/04 ,  B07C5/342 ,  G01N15/00

CPC classification number: G01N15/1429 ,  G01N15/1427 ,  G01N15/1459 ,  G01N2015/1488 ,  G01N2015/149 ,  Y10S209/906

Abstract: This invention relates to a method and apparatus for flexibly controlling sorting decisions for a flow cytometer or similar instrument at a purity vs. yield ratio of sorted particles, particularly at high event rates. The system monitors distances between sequential objects to determine if they are in close enough proximity to be within a single resolution unit of the sorting device. The "friend" or "foe" status of neighboring events is considered by this system in dealing with detected "coincident" events. The system operates on information of the desirability to sort the object, i.e. droplet, based upon the properties of the individual object. A storage and retrieval method is provided for making the information available at the time a sort operation based on a sorting logic condition is performed. Accordingly, an easily reconfigurable mode of operation control is provided to allow customized sorting strategies, based on the needs of individual applications or experiments. This system may be included as an integral part of a flow cytometer/cell sorter or similar instrument and may also be provided as an outboard module to such systems.

Abstract translation: 本发明涉及一种用于灵活地控制流式细胞仪或类似仪器的分选决定的方法和装置，其分选颗粒的纯度与产率之比，特别是在高事件率下。 系统监视顺序对象之间的距离，以确定它们是否处于足够接近的位置，处于分类设备的单个分辨率单位内。 该系统在处理检测到的“重合”事件时考虑了邻居事件的“朋友”或“敌方”状态。 该系统基于对单个对象的属性对物体（即液滴）进行分类的可取性的信息进行操作。 提供了一种存储和检索方法，用于在执行基于排序逻辑条件的分类操作时使信息可用。 因此，提供了一种易于重新配置的操作控制模式，以根据个别应用或实验的需要来允许定制的排序策略。 该系统可以作为流式细胞仪/细胞分选器或类似仪器的组成部分包括在内，也可以作为外部模块提供给这样的系统。

公开(公告)号：US5436921A

公开(公告)日：1995-07-25

申请号：US263854

申请日：1994-06-22

Applicant: Mark A. Corio

Inventor： Mark A. Corio

IPC: B41J2/44 ,  H01S5/06 ,  H01S5/062 ,  H01S5/40 ,  H04B10/145 ,  H04B10/155 ,  H01S3/10

CPC classification number: H04B10/50 ,  H01S5/4025 ,  H01S5/0617 ,  H01S5/06213 ,  Y10S372/703

Abstract: A high dynamic range laser diode modulation scheme employs two laser diodes connected so that their outputs are added in a single optical fiber. The first diode is modulated over its dynamic range. The second diode is also modulated over its dynamic range, and is attenuated with a filter to provide fine level adjustment to the coarse levels provided by the first diode.

Abstract translation: 高动态范围的激光二极管调制方案采用两个连接的激光二极管，使得它们的输出被添加到单个光纤中。 第一个二极管在其动态范围内被调制。 第二个二极管也在其动态范围内进行调制，并用滤波器衰减，以提供由第一二极管提供的粗略电平的精细电平调整。

公开(公告)号：US5204884A

公开(公告)日：1993-04-20

申请号：US671009

申请日：1991-03-18

Applicant: James F. Leary ,  Mark A. Corio ,  Scott R. McLaughlin

Inventor： James F. Leary ,  Mark A. Corio ,  Scott R. McLaughlin

IPC: G01N15/10 ,  B07C5/02 ,  B07C5/342 ,  G01N15/14 ,  G01N33/00 ,  G01N33/49

CPC classification number: B07C5/3425 ,  G01N15/1456 ,  G01N2015/149 ,  Y10S209/906 ,  Y10T436/10

Abstract: A system for sorting particles is arranged such that the presence or absence of multiple parameters is determined for each particle in a flow of particles, and a logic condition is determined based on the multiple parameters. Thereafter, particles are outputted from the system when the logic condition is present. Accordingly, multiparameter high-speed measurements of a rare subpopulation of particles (e.g. biological cells) can be made amidst a larger population of particles with differing characteristics. When attached to a multiparameter flow cytometer/cell sorter and microcomputer, the system allows multiparameter analysis of cells at rates in excess of 100,000 cells/sec. This system can be an outboard module attached to a commercially available or home-built flow cytometer.

Abstract translation: 排列粒子的系统是为了在粒子流中为每个粒子确定是否存在多个参数，并且基于多个参数来确定逻辑条件。 此后，当存在逻辑条件时，从系统输出粒子。 因此，可以在具有不同特征的更大群体的颗粒中制备罕见的亚群（例如生物细胞）的多参数高速测量。 当连接到多参数流式细胞仪/细胞分选器和微型计算机时，该系统允许以超过100,000个细胞/秒的速率对细胞进行多参数分析。 该系统可以是连接到市售或自制的流式细胞仪的外部模块。

公开(公告)号：US08731704B2

公开(公告)日：2014-05-20

申请号：US13041261

申请日：2011-03-04

Applicant: Mark A. Corio

Inventor： Mark A. Corio

IPC: D05B69/24

CPC classification number: D05B1/02 ,  D05B85/10 ,  D05C11/16

Abstract: A sewing device includes a material wrapped around a spool (the spool rotating around an axis), and a needle with a hole through which the material passes. A material feeding mechanism moves the needle and feeds the material in a feeding direction, thereby unraveling the material from the spool and stitching the material into a workpiece. A controller controls the material feeding mechanism to move the needle to a first rest position above a top surface of the workpiece. The first rest position is determined by the following formula: PR1=PW1+AT; wherein PR1 is the first rest position; PW1 is a first position of the top surface of the workpiece; and AT is an amount of material to be used in forming a second stitch in the workpiece after forming a first stitch in the workpiece.

Abstract translation: 缝纫装置包括缠绕在卷轴（卷轴绕轴旋转的）的材料，以及具有孔的针，材料通过该针。 材料供给机构使针移动并沿进给方向进给材料，从而将材料从卷轴解开并将材料缝合到工件中。 控制器控制材料馈送机构将针移动到工件顶表面上方的第一静止位置。 第一个休息位置由以下公式确定：PR1 = PW1 + AT; 其中PR1是第一静止位置; PW1是工件顶面的第一位置; AT是在工件中形成第一线圈之后在工件中形成第二线圈的材料量。

公开(公告)号：US5199576A

公开(公告)日：1993-04-06

申请号：US681559

申请日：1991-04-05

Applicant: Mark A. Corio ,  James F. Leary

Inventor： Mark A. Corio ,  James F. Leary

IPC: B07C5/00 ,  G01N15/10 ,  G01N15/14 ,  G01N33/49

CPC classification number: G01N15/1429 ,  G01N15/1427 ,  G01N15/1459 ,  G01N2015/1488 ,  G01N2015/149 ,  Y10S209/906

Abstract: This invention relates to a method and apparatus for flexibly controlling sorting decisions for a flow cytometer or similar instrument at a purity vs. yield ratio of sorted particles, particularly at high event rates. The system monitors distances between sequential objects to determine if they are in close enough proximity to be within a single resolution unit of the sorting device. The "friend" or "foe" status of neighboring events is considered by this system in dealing with detected "coincident" events. The system operates on information of the desirability to sort the object, i.e. droplet, based upon the properties of the individual object. A storage and retrieval method is provided for making the information available at the time a sort operation based on a sorting logic condition is performed. Accordingly, an easily reconfigurable mode of operation control is provided to allow customized sorting strategies, based on the needs of individual applications or experiments. This system may be included as an integral part of a flow cytometer/cell sorter or similar instrument and may also be provided as an outboard module to such systems.

Abstract translation: 本发明涉及一种用于灵活地控制流式细胞仪或类似仪器的分选决定的方法和装置，其分选颗粒的纯度与产率之间，特别是在高事件率下。 系统监视顺序对象之间的距离，以确定它们是否处于足够接近的位置，处于分类设备的单个分辨率单位内。 该系统在处理检测到的“重合”事件时考虑了邻居事件的“朋友”或“敌方”状态。 该系统基于对单个对象的属性对物体（即液滴）进行分类的可取性的信息进行操作。 提供了一种存储和检索方法，用于在执行基于排序逻辑条件的分类操作时使信息可用。 因此，提供了一种易于重新配置的操作控制模式，以根据个别应用或实验的需要来允许定制的排序策略。 该系统可以作为流式细胞仪/细胞分选器或类似仪器的组成部分包括在内，也可以作为外部模块提供给这样的系统。

公开(公告)号：US5804143A

公开(公告)日：1998-09-08

申请号：US702209

申请日：1996-08-23

Applicant: James F. Leary ,  Mark A. Corio ,  Scott R. McLaughlin

Inventor： James F. Leary ,  Mark A. Corio ,  Scott R. McLaughlin

IPC: G01N15/10 ,  B07C5/02 ,  B07C5/342 ,  G01N15/14 ,  G01N33/00 ,  G01N33/49

CPC classification number: B07C5/3425 ,  G01N15/1456 ,  G01N2015/149 ,  Y10S209/906 ,  Y10T436/10

Abstract: A system for sorting particles is arranged such that the presence or absence of multiple parameters is determined for each particle in a flow of particles, and a logic condition is determined based on the multiple parameters. Thereafter, particles are outputted from the system when the logic condition is present. Accordingly, multiparameter high-speed measurements of a rare subpopulation of particles (e.g. biological cells) can be made amidst a larger population of particles with differing characteristics. When attached to a multiparameter flow cytometer/cell sorter and microcomputer, the system allows multiparameter analysis of cells at rates in excess of 100,000 cells/sec. This system can be an outboard module attached to a commercially available or home-built flow cytometer.

Abstract translation: 排列粒子的系统是为了在粒子流中为每个粒子确定是否存在多个参数，并且基于多个参数来确定逻辑条件。 此后，当存在逻辑条件时，从系统输出粒子。 因此，可以在具有不同特征的更大群体的颗粒中制备罕见的亚群（例如生物细胞）的多参数高速测量。 当连接到多参数流式细胞仪/细胞分选器和微型计算机时，该系统允许以超过100,000个细胞/秒的速率对细胞进行多参数分析。 该系统可以是连接到市售或自制的流式细胞仪的外部模块。

公开(公告)号：US5374940A

公开(公告)日：1994-12-20

申请号：US710221

申请日：1991-06-04

Applicant: Mark A. Corio

Inventor： Mark A. Corio

IPC: G06F3/14 ,  H05K1/18 ,  G09G5/00

CPC classification number: G06F3/1438 ,  H05K1/189

Abstract: Multiple video boards of the general purpose type (e.g. EGA or VGA boards) and their associated monitors are operated by a single computer of the personal computer type, without modification of the computer hardware and while the video boards reside in their usual expansion slots which are provided by connectors on the mother board of the personal computer by a system which utilizes a flexible printed circuit board containing circuits which allows the processor (CPU) of the computer to present address and data signals which operate circuitry on the flexible boards to selectively enable or inhibit the communication of control signals (on the I/O and memory read and write control lines) to the video boards thereby enabling them to generate graphical images for their associated monitors. The signals on the address control and data lines are tapped and relayed either directly or after processing to the associated video board thereby enabling multiple video boards of the same or different types to be used concurrently by appropriate programming of the computer without the need to interchange hardware to switch between displays thereby reducing the inconvenience and potential hardware damage necessarily incident to such a hardware interchange procedure.

Abstract translation: 通用型（例如EGA或VGA板）和其相关监视器的多个视频板由个人计算机类型的单个计算机操作，而不修改计算机硬件，而视频板驻留在其通常的扩展槽 由个人计算机母板上的连接器提供，该系统利用包含电路的柔性印刷电路板，该电路允许计算机的处理器（CPU）呈现在柔性板上操作电路的地址和数据信号，以选择性地启用或 禁止控制信号（在I / O和存储器读写控制线上）与视频板的通信，从而使它们能够为其相关监视器生成图形图像。 地址控制和数据线上的信号可以直接地或在处理之后被中继到相关联的视频板，从而使得能够通过对计算机的适当编程并行地使用相同或不同类型的多个视频板，而不需要交换硬件 在显示器之间进行切换，从而减少必然事件对这种硬件交换过程的不便和潜在的硬件损坏。