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1.
公开(公告)号:US06822142B2
公开(公告)日:2004-11-23
申请号:US09885723
申请日:2001-06-20
申请人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
IPC分类号: C12N1509
CPC分类号: C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要: Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译: 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞,植物,种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶,至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞,植物,种子和储存器官。
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2.
公开(公告)号:US07544863B2
公开(公告)日:2009-06-09
申请号:US10862907
申请日:2004-06-07
申请人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
CPC分类号: C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要: Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译: 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞,植物,种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶,至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞,植物,种子和储存器官。
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公开(公告)号:US20060107350A1
公开(公告)日:2006-05-18
申请号:US11338399
申请日:2006-01-24
CPC分类号: C12N15/8247 , C12N9/1029
摘要: Nucleic acids are disclosed that encode fatty acid β-keto acyl synthases from plants. Such synthases are effective for producing very long chain fatty acids (VLCFA), e.g., C22 to C26, preferentially saturated but also monounsaturated. Also disclosed are polypeptides encoded by such nucleic acids. Transgenic plants expressing these polypeptides exhibit altered levels of VLCFA in one or more tissues, such as seeds or leaves.
摘要翻译: 公开了从植物编码脂肪酸β-酮酰基合成酶的核酸。 这种合酶对于生产非常长链脂肪酸(VLCFA)是有效的,例如C22至C26,优选饱和但也是单不饱和的。 还公开了由这种核酸编码的多肽。 表达这些多肽的转基因植物在一个或多个组织例如种子或叶中表现出VLCFA水平的变化。
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4.
公开(公告)号:US20050086713A1
公开(公告)日:2005-04-21
申请号:US10862907
申请日:2004-06-07
申请人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh Kishore , Gregory Thorne , John LeDeaux
发明人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh Kishore , Gregory Thorne , John LeDeaux
CPC分类号: C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要: Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译: 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞,植物,种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶,至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞,植物,种子和储存器官。
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5.
公开(公告)号:US07906710B2
公开(公告)日:2011-03-15
申请号:US12477673
申请日:2009-06-03
申请人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
CPC分类号: C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要: Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译: 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞,植物,种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶,至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞,植物,种子和储存器官。
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公开(公告)号:US20100037354A1
公开(公告)日:2010-02-11
申请号:US12477673
申请日:2009-06-03
申请人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
发明人: Balasulojini Karunanandaa , Martha Post-Beittenmiller , Mylavarapu Venkatramesh , Ganesh M. Kishore , Gregory M. Thorne , John R. LeDeaux
CPC分类号: C12N9/0073 , C12N9/0006 , C12N9/0083 , C12N9/1007 , C12N15/8243 , C12N15/8247 , C12Y101/01034 , C12Y114/99007
摘要: Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
摘要翻译: 公开了包含编码3-羟基-3甲基戊二酰辅酶A还原酶和至少一种其它甾醇合成途径酶的序列的构建体。 还公开了使用这种构建体来改变植物的细胞,植物,种子和储存器官中的固醇产生和含量的方法。 还提供了含有通过使用所公开的构建体产生的改变的固醇水平的油和组合物。 还提供了可用于改变甾醇生产的新型核苷酸序列。 还提供了包含编码3-羟基-3甲基戊二酰辅酶A还原酶,至少一种其它甾醇合成途径酶和至少一种生育酚合成酶的序列的植物的细胞,植物,种子和储存器官。
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