公开(公告)号：US5981202A

公开(公告)日：1999-11-09

申请号：US397973

申请日：1995-03-03

申请人： Masayuki Masuko

发明人： Masayuki Masuko

IPC分类号： C12M1/34 ,  G01N21/76 ,  G01N33/543 ,  G01N33/58 ,  G01N33/53 ,  G01N33/551

CPC分类号： G01N33/582 ,  G01N21/76 ,  G01N33/54313 ,  Y10S435/962 ,  Y10S435/968 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S436/809

摘要： A method for assaying a substance is provided in which an optical image is obtained from spots of light emitted from a chemiluminescent material capable of undergoing a luminescent reaction. The method comprises: (a) a step of distributing solid particles on the surface of a support, the solid particles having a diameter of not more than 100 .mu.m and on its surface carrying an antibody being able to catch a catalyst previously bound to the second antibody (or its analogue, such as Fab') through binding an antigen, and the support being permeable to a liquid and capable of absorbing light emitted from chemiluminescent material; (b) a step of immersing the support into a solution containing the chemiluminescent material to bring the catalyst over the support into contact with the chemiluminescent material to cause luminescent reactions including fluorescence; (c) a step of two-dimensionally picking up an optical image including an image formed by luminescence and fluorescence, and converting it into electric signals; (d) a step of storing the measured two-dimensional image data of the optical image in a image processing device; and (e) a step of manipulating the stored signals to form a new image in the same processing device.

摘要翻译： 提供了一种用于测定物质的方法，其中从能够经历发光反应的化学发光材料发射的光斑获得光学图像。 该方法包括：（a）将固体颗粒分布在载体表面上的步骤，固体颗粒的直径不超过100μm，并且其表面上携带抗体能够捕获先前与 第二抗体（或其类似物，例如Fab'）通过结合抗原，并且所述载体可渗透液体并且能够吸收从化学发光材料发射的光; （b）将载体浸入含有化学发光材料的溶液中以使催化剂在载体上与化学发光材料接触以引起包括荧光在内的发光反应的步骤; （c）二维地拾取包含由发光和荧光形成的图像的光学图像，并将其转换为电信号的步骤; （d）将所测量的光学图像的二维图像数据存储在图像处理装置中的步骤; 和（e）在相同的处理装置中操纵存储的信号以形成新图像的步骤。

公开(公告)号：US5637874A

公开(公告)日：1997-06-10

申请号：US393387

申请日：1995-02-23

申请人： Katsu Honzawa ,  Kazuhiro Atsumi ,  Fumihiko Shimomura ,  Masayuki Masuko ,  Tsuyoshi Hayakawa

发明人： Katsu Honzawa ,  Kazuhiro Atsumi ,  Fumihiko Shimomura ,  Masayuki Masuko ,  Tsuyoshi Hayakawa

IPC分类号： G01N21/75 ,  C12M1/34 ,  G01N21/03 ,  G01N21/76

CPC分类号： G01N21/76

摘要： The present invention provides a chemiluminescence measuring apparatus which is small in size and high in operability and has a function of performing accurate quantitative analysis and the like. The apparatus includes at least a box-like housing having an opening, a cover provided on a top surface of the housing and having a through hole for introducing a first detachable vessel into the inner space of the housing, a holder having a concave portion and enclosing the opening of the housing, the hollow chamber having an opening at a side wall thereof and partially housing a second vessel, a photo-sensing unit for detecting luminescence from the second vessel via the through hole of the hollow chamber, and a shutter mechanism for intermittently cutting optical path between the photo-sensing unit and the second vessel. Particularly, the shutter mechanism includes a rotatable hollow chamber partially housing the vessel, provided in the hollow chamber and having an opening at a side wall thereof, and a driving motor rotating the rotatable hollow chamber.

摘要翻译： 本发明提供一种尺寸小，操作性高的化学发光测定装置，具有进行准确的定量分析等功能。 该装置至少包括具有开口的盒状壳体，设置在壳体的顶表面上的盖，并具有用于将第一可拆卸容器引入壳体的内部空间的通孔，具有凹部和 封闭壳体的开口，中空室在其侧壁处具有开口并且部分地容纳第二容器，用于经由中空室的通孔检测来自第二容器的发光的光感测单元，以及快门机构 用于间歇地切割感光单元和第二容器之间的光路。 特别地，闸门机构包括：可旋转的中空室，其设置在中空室中，部分地容纳容器，并且在其侧壁具有开口;以及驱动马达，其旋转可旋转中空室。

公开(公告)号：US5523845A

公开(公告)日：1996-06-04

申请号：US378369

申请日：1995-01-25

申请人： Katsu Honzawa ,  Kazuhiro Atsumi ,  Humihiko Shimomura ,  Seiji Kawaguchi ,  Yuichiro Sakamoto ,  Hisaya Motojima ,  Masayuki Masuko ,  Tsuyoshi Hayakawa

发明人： Katsu Honzawa ,  Kazuhiro Atsumi ,  Humihiko Shimomura ,  Seiji Kawaguchi ,  Yuichiro Sakamoto ,  Hisaya Motojima ,  Masayuki Masuko ,  Tsuyoshi Hayakawa

IPC分类号： G01N21/63 ,  G01N1/00 ,  G01N21/15 ,  G01N21/17 ,  G01N35/10 ,  G01N21/11 ,  G01N21/64 ,  G01N21/65

CPC分类号： G01N21/15 ,  G01N2035/1062

摘要： The first branch 1a of the optical fiber 1 is connected to the light source 2, and the second branch 1b is connected to the optical measuring unit 3. A taper-shaped tip member 8 is fitted to a tip end of the optical fiber unit 1 with the use of a jig member 4. The inner hollow space 7 in the tip member 8 is connected with the air suction device 11, through the gap 5 formed between the optical fiber unit 1 and the jig member 4 and the air suction tube 10. The air suction device 11 discharges air from the inner hollow space 7 to suck liquid through the through-hole 6 into the inner hollow space. The air suction device 11 is controlled by the controller 12. Because the optical fiber unit can directly irradiate liquid with light, measurement can be performed with high sensitivity. The optical fiber unit is prevented from contamination.

摘要翻译： 光纤1的第一分支1a连接到光源2，第二分支1b连接到光学测量单元3.锥形尖端构件8装配到光纤单元1的末端 通过使用夹具构件4.通过形成在光纤单元1和夹具构件4之间的间隙5和吸气管10，尖端构件8中的内部中空空间7与吸气装置11连接 吸气装置11从内部中空空间7排出空气，通过通孔6将液体吸入内部中空空间。 吸气装置11由控制器12控制。由于光纤单元可以直接用光照射液体，因此可以以高灵敏度进行测量。 防止光纤单元的污染。