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公开(公告)号：US20110117615A1
公开(公告)日：2011-05-19
申请号：US12920007
申请日：2009-02-27
Applicant: Edward Green , Matthew Crow
Inventor： Edward Green , Matthew Crow
Abstract: The invention relates to a process for producing organic acids, acid derivatives thereof and/or organic alcohols. In particular, the invention relates to a process for culturing a butyric acid-producing micro-organism in a culture vessel; transferring a portion of the butyric acid which is produced in the culture vessel to a separate compartment; and then reintroducing a portion of the acids from the second compartment in a controlled manner into the culture vessel. The invention also relates to a process for making butanol. The invention further relates to a process for culturing a butyrate-producing micro-organism in a first compartment of an electrochemical reactor, passing a direct current electric field across the electrochemical reactor and harvesting butyric acid or acid derivatives thereof in a second compartment of the reactor. The invention further relates to a process for producing butanol from the butyric acid.
Abstract translation: 本发明涉及一种生产有机酸，其酸衍生物和/或有机醇的方法。 特别地，本发明涉及一种在培养容器中培养产生丁酸的微生物的方法; 将在培养容器中产生的一部分丁酸转移到单独的隔室; 然后以受控方式将来自第二隔室的一部分酸重新引入培养容器中。 本发明还涉及制备丁醇的方法。 本发明还涉及一种在电化学反应器的第一隔室中培养产生丁酸盐的微生物的方法，使直流电场穿过电化学反应器并在反应器的第二隔室中收获其丁酸或其酸衍生物 。 本发明还涉及从丁酸生产丁醇的方法。
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公开(公告)号：US20060154247A1
公开(公告)日：2006-07-13
申请号：US10516204
申请日：2003-06-02
Applicant: Matthew Baker , Matthew Crow
Inventor： Matthew Baker , Matthew Crow
CPC classification number: C12N15/1006 , C12N15/1003
Abstract: Methods, compositions and kits for concentrating or separating cells containing target nucleic acid are disclosed, especially m mixtures containing the cells and other components such as impurities. The methods can keep a large proportion of the cells intact, allowing the cells to be employed after separation (e.g. cultured) and/or which facilitates the recovery of nucleic acid from the cells. The method employs flocculating agents, such as polyamines or cationic detergents, to form complexes with cells causing them to aggregate and so separated from other components of the mixture. Conveniently, the separation of the aggregated cells can be effected with a solid phase which is capable of binding the cells, such as magnetic beads or filters.
Abstract translation: 公开了用于浓缩或分离含有靶核酸的细胞的方法，组合物和试剂盒，特别是含有细胞和其它成分如杂质的混合物。 所述方法可以保持大部分细胞完整，允许在分离（例如培养）后使用细胞和/或有助于从细胞中回收核酸。 该方法使用絮凝剂，例如多胺或阳离子洗涤剂，与细胞形成复合物，导致它们与混合物的其它组分聚集并分离。 方便地，聚集的细胞的分离可以用能够结合诸如磁珠或过滤器的细胞的固相来实现。
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公开(公告)号：US09249431B2
公开(公告)日：2016-02-02
申请号：US12920007
申请日：2009-02-27
Applicant: Edward Green , Matthew Crow
Inventor： Edward Green , Matthew Crow
Abstract: The invention relates to a process for producing organic acids, acid derivatives thereof and/or organic alcohols. In particular, the invention relates to a process for culturing a butyric acid-producing micro-organism in a culture vessel; transferring a portion of the butyric acid which is produced in the culture vessel to a separate compartment; and then reintroducing a portion of the acids from the second compartment in a controlled manner into the culture vessel. The invention also relates to a process for making butanol. The invention further relates to a process for culturing a butyrate-producing micro-organism in a first compartment of an electrochemical reactor, passing a direct current electric field across the electrochemical reactor and harvesting butyric acid or acid derivatives thereof in a second compartment of the reactor. The invention further relates to a process for producing butanol from the butyric acid.
Abstract translation: 本发明涉及一种生产有机酸，其酸衍生物和/或有机醇的方法。 特别地，本发明涉及一种在培养容器中培养产生丁酸的微生物的方法; 将在培养容器中产生的一部分丁酸转移到单独的隔室; 然后以受控方式将来自第二隔室的一部分酸重新引入培养容器中。 本发明还涉及制备丁醇的方法。 本发明还涉及一种在电化学反应器的第一隔室中培养产生丁酸盐的微生物的方法，使直流电场穿过电化学反应器并在反应器的第二隔室中收获其丁酸或其酸衍生物 。 本发明还涉及从丁酸生产丁醇的方法。
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