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    IRES functioning in plant
    1.
    发明授权
    IRES functioning in plant 失效
    IRES在植物中运作

    公开(公告)号：US08772465B2

    公开(公告)日：2014-07-08

    申请号：US10586052

    申请日：2005-01-13

    申请人： Minami Matsui ,  Yoshiharu Yamamoto ,  Kazuhito Gohda ,  Kumiko Suzuki

    发明人： Minami Matsui ,  Yoshiharu Yamamoto ,  Kazuhito Gohda ,  Kumiko Suzuki

    IPC分类号： C12N15/82 ,  C07H21/04 ,  A01H5/00

    CPC分类号： C12N15/8216

    摘要： It is intended to provide for a polycistronic expression pattern in a plant used as a host. The present invention provides a polynucleotide which comprises the following DNA (a) or (b) and functions as an IRES (internal ribosome entry site) in a plant: (a) DNA of the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4; or (b) DNA of a nucleotide sequence derived from the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4 by the substitution, deletion, addition, and insertion of one or more bases and having a function of positively regulating the translation of a nucleic acid located downstream thereof.

    摘要翻译： 旨在提供用作宿主的植物中的多顺反子表达模式。 本发明提供了包含以下DNA（a）或（b）并在植物中用作IRES（内部核糖体进入位点）的多核苷酸：（a）由SEQ ID NO：1,2所示的核苷酸序列的DNA ，3或4; 或（b）通过一个或多个碱基的取代，缺失，添加和插入衍生自SEQ ID NO：1,2,3或4所示核苷酸序列的核苷酸序列的DNA，并且具有正调节功能 位于其下游的核酸的翻译。

    RICE GENE CAPABLE OF IMPARTING WIDE-SPECTRUM DISEASE RESISTANCE
    3.
    发明申请
    RICE GENE CAPABLE OF IMPARTING WIDE-SPECTRUM DISEASE RESISTANCE 有权
    能弥补广谱抗病性的米糠基因

    公开(公告)号：US20110258737A1

    公开(公告)日：2011-10-20

    申请号：US13061396

    申请日：2009-03-04

    申请人： Masaki Mori ,  Nagao Hayashi ,  Shoji Sugano ,  Hiroshi Takatsuji ,  Hirohiko Hirochika ,  Kenji Oda ,  Minami Matsui

    发明人： Masaki Mori ,  Nagao Hayashi ,  Shoji Sugano ,  Hiroshi Takatsuji ,  Hirohiko Hirochika ,  Kenji Oda ,  Minami Matsui

    IPC分类号： A01H5/00 ,  C12Q1/02 ,  C12N15/82 ,  C12N5/10

    CPC分类号： C12N15/8281 ,  C12N9/12 ,  C12N15/8282 ,  G01N33/5097 ,  G01N2333/195 ,  G01N2500/10

    摘要： A three-step screening of selection for resistance against pathogenic bacteria infection, selection for resistance against pathogenic fungi infection, and selection for sensitivity to salicylic acid was carried out on Arabidopsis thaliana lines highly expressing rice full-length cDNA (rice-FOX Arabidopsis lines) which were prepared by using a FOX hunting system. As a result, one Arabidopsis thaliana line (line K15424) selected in all of the three types of screening was successfully selected. Line K15424 carries a rice full-length cDNA. Rice overexpressing AK070024 was produced, and resistance against rice bacterial leaf blight was assayed using the T1 generation. As a result, it was confirmed that AK070024-overexpressing rice is resistant against bacterial leaf blight and blast.

    摘要翻译： 对高度表达水稻全长cDNA（水稻 - FOX拟南芥品系）的拟南芥系进行了三步筛选抗病原菌感染选择，抗病原真菌感染选择和对水杨酸敏感性的选择， 这是通过使用FOX狩猎系统准备的。 结果，在三种筛选中选出的一种拟南芥系（K15424系）成功筛选出来。 K15424系携带全长cDNA。 产生过表达AK070024的水稻，利用T1代测定了抗稻瘟病叶枯病的抗性。 结果证实，AK070024-过表达的稻对抗细菌性叶枯病和爆炸是抗性的。

    Ires Functioning In Plant
    4.
    发明申请
    Ires Functioning In Plant 失效
    Ires在植物中的功能

    公开(公告)号：US20080057581A1

    公开(公告)日：2008-03-06

    申请号：US10586052

    申请日：2005-01-13

    申请人： Minami Matsui ,  Yoshiharu Yamamoto ,  Kazuhito Gohda ,  Kumiko Suzuki

    发明人： Minami Matsui ,  Yoshiharu Yamamoto ,  Kazuhito Gohda ,  Kumiko Suzuki

    IPC分类号： A01H5/00 ,  C07H21/04 ,  C12N15/00 ,  C12N5/04

    CPC分类号： C12N15/8216

    摘要： It is intended to provide for a polycistronic expression pattern in a plant used as a host. The present invention provides a polynucleotide which comprises the following DNA (a) or (b) and functions as IRES (internal ribosome entry site) in a plant: (a) DNA of the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4; or (b) DNA of a nucleotide sequence derived from the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4 by the substitution, deletion, addition, and insertion of one or more bases and having a function of positively regulating the translation of a gene located downstream along the translation direction in the plant.

    摘要翻译： 旨在提供用作宿主的植物中的多顺反子表达模式。 本发明提供了包含以下DNA（a）或（b）并在植物中作为IRES（内部核糖体进入位点）起作用的多核苷酸：（a）SEQ ID NO：1,2所示核苷酸序列的DNA， 3或4; 或（b）通过一个或多个碱基的取代，缺失，添加和插入衍生自SEQ ID NO：1,2,3或4所示核苷酸序列的核苷酸序列的DNA，并且具有正调节功能 在植物中沿着翻译方向位于下游的基因的翻译。

    Plant system for comprehensive gene function analysis with the use of full-length cDNA
    9.
    发明授权
    Plant system for comprehensive gene function analysis with the use of full-length cDNA 失效
    使用全长cDNA进行综合基因功能分析的植物系统

    公开(公告)号：US07608758B2

    公开(公告)日：2009-10-27

    申请号：US10488087

    申请日：2002-08-29

    申请人： Minami Matsui ,  Takanari Ichikawa ,  Miki Nakazawa ,  Motoaki Seki ,  Miki Fujita ,  Kazuo Shinozaki

    发明人： Minami Matsui ,  Takanari Ichikawa ,  Miki Nakazawa ,  Motoaki Seki ,  Miki Fujita ,  Kazuo Shinozaki

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8216

    摘要： A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.

    摘要翻译： 一个易于指定表型的基因，从而全面分析基因功能。 用于分析基因功能的方法包括（a）用含有表达调控序列的全长cDNA文库感染植物组，以将cDNA引入植物中; （b）从已经引入cDNA的植物中选择符合所需选择要求的植物; （c）从所选择的植物中分离cDNA; 和（d）将如此分离的cDNA重新引入与引入cDNA的植物相同物种的植物中，以根据选择要求重新确认表型。