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    Plant system for comprehensive gene function analysis with the use of full-length cDNA
    3.
    发明授权
    Plant system for comprehensive gene function analysis with the use of full-length cDNA 失效
    使用全长cDNA进行综合基因功能分析的植物系统

    公开(公告)号：US07608758B2

    公开(公告)日：2009-10-27

    申请号：US10488087

    申请日：2002-08-29

    申请人： Minami Matsui ,  Takanari Ichikawa ,  Miki Nakazawa ,  Motoaki Seki ,  Miki Fujita ,  Kazuo Shinozaki

    发明人： Minami Matsui ,  Takanari Ichikawa ,  Miki Nakazawa ,  Motoaki Seki ,  Miki Fujita ,  Kazuo Shinozaki

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8216

    摘要： A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.

    摘要翻译： 一个易于指定表型的基因，从而全面分析基因功能。 用于分析基因功能的方法包括（a）用含有表达调控序列的全长cDNA文库感染植物组，以将cDNA引入植物中; （b）从已经引入cDNA的植物中选择符合所需选择要求的植物; （c）从所选择的植物中分离cDNA; 和（d）将如此分离的cDNA重新引入与引入cDNA的植物相同物种的植物中，以根据选择要求重新确认表型。

    Plant Dwarfing Gene
    8.
    发明申请
    Plant Dwarfing Gene 审中-公开
    植物矮化基因

    公开(公告)号：US20080047030A1

    公开(公告)日：2008-02-21

    申请号：US10571173

    申请日：2004-09-10

    申请人： Minami Matsui ,  Miki Nakazawa ,  Takanari Ichikawa ,  Shu Muto

    发明人： Minami Matsui ,  Miki Nakazawa ,  Takanari Ichikawa ,  Shu Muto

    IPC分类号： A01H5/00 ,  C07H21/04 ,  C07K14/00 ,  C12N15/00 ,  C12N15/82

    CPC分类号： C07K14/415 ,  C12N15/8261 ,  Y02A40/146

    摘要： An object of the present invention is to explore, isolate, and identify a dwarfing gene by activation tagging. The present invention provides a gene encoding a protein of the following (a) or (b): (a) a protein consisting of the amino acid sequence represented by any of SEQ ID NOs: 2, 4, 6, and 8; or (b) a protein consisting of an amino acid sequence derived from the amino acid sequence represented by any of SEQ ID NOs: 2, 4, 6, and 8 with the deletion, substitution, or addition of one or several amino acids and having the activity of dwarfing a plant body.

    摘要翻译： 本发明的目的是通过激活标记来探索，分离和鉴定矮化基因。 本发明提供编码以下（a）或（b）的蛋白质的基因：（a）由SEQ ID NO：2,4,6,8所示的氨基酸序列组成的蛋白质; 或（b）由SEQ ID NO：2,4,6,8所示的氨基酸序列的氨基酸序列组成的蛋白质，其中缺失，取代或添加一个或数个氨基酸，并具有 矮化植物体的活动。

    IRES functioning in plant
    10.
    发明授权
    IRES functioning in plant 失效
    IRES在植物中运作

    公开(公告)号：US08772465B2

    公开(公告)日：2014-07-08

    申请号：US10586052

    申请日：2005-01-13

    申请人： Minami Matsui ,  Yoshiharu Yamamoto ,  Kazuhito Gohda ,  Kumiko Suzuki

    发明人： Minami Matsui ,  Yoshiharu Yamamoto ,  Kazuhito Gohda ,  Kumiko Suzuki

    IPC分类号： C12N15/82 ,  C07H21/04 ,  A01H5/00

    CPC分类号： C12N15/8216

    摘要： It is intended to provide for a polycistronic expression pattern in a plant used as a host. The present invention provides a polynucleotide which comprises the following DNA (a) or (b) and functions as an IRES (internal ribosome entry site) in a plant: (a) DNA of the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4; or (b) DNA of a nucleotide sequence derived from the nucleotide sequence represented by SEQ ID NO: 1, 2, 3, or 4 by the substitution, deletion, addition, and insertion of one or more bases and having a function of positively regulating the translation of a nucleic acid located downstream thereof.

    摘要翻译： 旨在提供用作宿主的植物中的多顺反子表达模式。 本发明提供了包含以下DNA（a）或（b）并在植物中用作IRES（内部核糖体进入位点）的多核苷酸：（a）由SEQ ID NO：1,2所示的核苷酸序列的DNA ，3或4; 或（b）通过一个或多个碱基的取代，缺失，添加和插入衍生自SEQ ID NO：1,2,3或4所示核苷酸序列的核苷酸序列的DNA，并且具有正调节功能 位于其下游的核酸的翻译。