摘要:
An object of the present invention is to provide a plant having enhanced resistance to environmental stress by controlling the expression of a histone modifying enzyme in a plant. The present invention relates to a method of producing a plant having enhanced resistance to environmental stress, comprising a step of suppressing or increasing the expression of a histone deacetylase gene.
摘要:
The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.
摘要翻译:本发明提供一种通过(a)用具有SEQ ID NO:27的非生物环境胁迫应答启动子和其下游基因的表达载体制备重组植物细胞系,植物组织或植物来调节基因表达的方法 ; 和(b)在非生物环境胁迫下培养和培养重组植物细胞,植物组织或植物,其中所述启动子调节所述基因在非生物环境胁迫下的表达。
摘要:
An object of the present invention is to provide a plant having enhanced resistance to environmental stress by controlling the expression of a histone modifying enzyme in a plant. The present invention relates to a method of producing a plant having enhanced resistance to environmental stress, comprising a step of suppressing or increasing the expression of a histone deacetylase gene.
摘要:
The present invention provides a stress responsive promoter. The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c):(a) DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; (b) DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.
摘要翻译:本发明提供一种应激反应启动子。 本发明的环境胁迫响应启动子包含以下(a),(b)或(c)的DNA:(a)由选自SEQ ID NO:1至90的任何核苷酸序列组成的DNA; (b)由相对于选自SEQ ID NO:1至90的任何核苷酸序列缺失,取代或添加一个或多个核苷酸的核苷酸序列组成的DNA,并且起到环境胁迫响应启动子的作用; 和(c)在严格条件下与由选自SEQ ID NO:1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。
摘要:
The present invention provides an early-maturing transgenic plant, which comprises a nucleic acid that encodes a protein having activity of causing early maturation of a plant, so that the gene can be expressed.
摘要:
The present invention provides an early-maturing transgenic plant, which comprises a nucleic acid that encodes a protein having activity of causing early maturation of a plant, so that the gene can be expressed.
摘要:
This invention concerns induction of gene expression specifically in given tissue with the use of a novel promoter that has environmental stress responsiveness and functions specifically in given tissue. It is realized by a method comprising: a step of preparing a plant having an arbitrary gene downstream of the environmental stress responsive promoter comprising DNA (a), (b), or (c) below; (a) DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8; (b) DNA comprising a nucleotide sequence derived from the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 by deletion, substitution, or addition of one or a plurality of nucleotides and functioning as an environmental stress responsive promoter; or (c) DNA hybridizing under stringent conditions to DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 and functioning as an environmental stress responsive promoter; and a step of cultivating the plant in the presence of environmental stress, the method comprising inducing expression of the gene located downstream of the environmental stress responsive promoter in a tissue-specific manner.
摘要翻译:本发明涉及使用具有环境应激反应性和特定于给定组织中功能的新型启动子来特异性诱导给定组织中的基因表达。 通过以下方法实现:包括下述DNA(a),(b)或(c)的环境胁迫响应启动子下游具有任意基因的植物的步骤; (a)包含SEQ ID NO:1至8中任一个所示的核苷酸序列的DNA; (b)通过缺失,取代或添加一个或多个核苷酸并起到环境应激反应启动子的作用,其包含衍生自SEQ ID NO:1至8中任一项所示核苷酸序列的核苷酸序列的DNA; 或(c)在严格条件下与包含SEQ ID NO:1至8中任一所示的核苷酸序列的DNA杂交并用作环境胁迫响应启动子的DNA的DNA; 以及在存在环境胁迫的情况下培育植物的步骤,所述方法包括以组织特异性方式诱导位于环境胁迫响应启动子下游的基因的表达。
摘要:
A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.
摘要:
The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.
摘要:
The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c): DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.
摘要翻译:本发明的环境胁迫响应启动子包含以下(a),(b)或(c)的DNA:由选自SEQ ID NO:1〜90的任意碱基序列组成的DNA; 由包含相对于选自SEQ ID NO:1至90的任何核苷酸序列的一个或多个核苷酸的缺失,取代或添加的核苷酸序列组成的DNA,并且起到环境应激反应启动子的作用; 和(c)在严格条件下与由选自SEQ ID NO:1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。