公开(公告)号：US07608758B2

公开(公告)日：2009-10-27

申请号：US10488087

申请日：2002-08-29

申请人： Minami Matsui ,  Takanari Ichikawa ,  Miki Nakazawa ,  Motoaki Seki ,  Miki Fujita ,  Kazuo Shinozaki

发明人： Minami Matsui ,  Takanari Ichikawa ,  Miki Nakazawa ,  Motoaki Seki ,  Miki Fujita ,  Kazuo Shinozaki

IPC分类号： C12N15/82

CPC分类号： C12N15/8216

摘要： A gene causative of a phenotype is easily specified and thus gene functions are comprehensively analyzed. A method for analyzing a gene function includes (a) infecting a plant group with a full-length cDNA library containing an expression regulatory sequence to introduce the cDNA into the plants; (b) selecting a plant complying with desired selection requirements from the plants into which the cDNA has been introduced; (c) isolating the cDNA from the selected plant; and (d) re-introducing the thus isolated cDNA into a plant of the same species as the plant into which the cDNA has been introduced, to reconfirm a phenotype according to the selection requirements.

摘要翻译： 一个易于指定表型的基因，从而全面分析基因功能。 用于分析基因功能的方法包括（a）用含有表达调控序列的全长cDNA文库感染植物组，以将cDNA引入植物中; （b）从已经引入cDNA的植物中选择符合所需选择要求的植物; （c）从所选择的植物中分离cDNA; 和（d）将如此分离的cDNA重新引入与引入cDNA的植物相同物种的植物中，以根据选择要求重新确认表型。

公开(公告)号：US20080216196A1

公开(公告)日：2008-09-04

申请号：US12076932

申请日：2008-03-25

申请人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

发明人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

IPC分类号： C12N15/29 ,  C12N15/82

CPC分类号： C12N15/8238 ,  C07K14/415 ,  C12N15/8237 ,  C12N15/8273

摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

摘要翻译： 本发明提供一种通过（a）用具有SEQ ID NO：27的非生物环境胁迫应答启动子和其下游基因的表达载体制备重组植物细胞系，植物组织或植物来调节基因表达的方法 ; 和（b）在非生物环境胁迫下培养和培养重组植物细胞，植物组织或植物，其中所述启动子调节所述基因在非生物环境胁迫下的表达。

公开(公告)号：US20070094752A1

公开(公告)日：2007-04-26

申请号：US11470154

申请日：2006-09-05

申请人： Kazuo SHINOZAKI ,  Motoaki Seki ,  Miki Fujita

发明人： Kazuo SHINOZAKI ,  Motoaki Seki ,  Miki Fujita

IPC分类号： C12N15/82

CPC分类号： C12N15/8238 ,  C07K14/415 ,  C12N15/8237 ,  C12N15/8273

摘要： The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c): DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.

摘要翻译： 本发明的环境胁迫响应启动子包含以下（a），（b）或（c）的DNA：由选自SEQ ID NO：1〜90的任意碱基序列组成的DNA; 由包含相对于选自SEQ ID NO：1至90的任何核苷酸序列的一个或多个核苷酸的缺失，取代或添加的核苷酸序列组成的DNA，并且起到环境应激反应启动子的作用; 和（c）在严格条件下与由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。

公开(公告)号：US20050009187A1

公开(公告)日：2005-01-13

申请号：US10495918

申请日：2002-11-15

申请人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

发明人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

IPC分类号： A01H5/00 ,  C07K14/415 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/82 ,  C07H21/04 ,  C12N15/63 ,  C12N15/87

CPC分类号： C07K14/415 ,  C12N15/8237 ,  C12N15/8238 ,  C12N15/8273

摘要： The present invention provides a stress responsive promoter. The environmental stress responsive promoter of the present invention comprises DNA of the following (a), (b) or (c):(a) DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90; (b) DNA consisting of a nucleotide sequence comprising a deletion, substitution or addition of one or more nucleotides relative to any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter; and (c) DNA hybridizing under stringent conditions to DNA consisting of any nucleotide sequence selected from SEQ ID NOS: 1 to 90, and functioning as an environmental stress responsive promoter.

摘要翻译： 本发明提供一种应激反应启动子。 本发明的环境胁迫响应启动子包含以下（a），（b）或（c）的DNA：（a）由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA; （b）由相对于选自SEQ ID NO：1至90的任何核苷酸序列缺失，取代或添加一个或多个核苷酸的核苷酸序列组成的DNA，并且起到环境胁迫响应启动子的作用; 和（c）在严格条件下与由选自SEQ ID NO：1至90的任何核苷酸序列组成的DNA杂交并用作环境胁迫响应启动子的DNA。

公开(公告)号：US20090282582A1

公开(公告)日：2009-11-12

申请号：US12311900

申请日：2007-10-19

申请人： Motoaki Seki ,  Jong-Myong Kim ,  Kazuo Shinozaki ,  Miki Fujita

发明人： Motoaki Seki ,  Jong-Myong Kim ,  Kazuo Shinozaki ,  Miki Fujita

IPC分类号： C12N15/82 ,  C07H21/04 ,  C12N15/63 ,  A01H5/00 ,  C12N5/10

CPC分类号： C07K14/415 ,  C12N15/8237

摘要： This invention concerns induction of gene expression specifically in given tissue with the use of a novel promoter that has environmental stress responsiveness and functions specifically in given tissue. It is realized by a method comprising: a step of preparing a plant having an arbitrary gene downstream of the environmental stress responsive promoter comprising DNA (a), (b), or (c) below; (a) DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8; (b) DNA comprising a nucleotide sequence derived from the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 by deletion, substitution, or addition of one or a plurality of nucleotides and functioning as an environmental stress responsive promoter; or (c) DNA hybridizing under stringent conditions to DNA comprising the nucleotide sequence as shown in any of SEQ ID NO: 1 to 8 and functioning as an environmental stress responsive promoter; and a step of cultivating the plant in the presence of environmental stress, the method comprising inducing expression of the gene located downstream of the environmental stress responsive promoter in a tissue-specific manner.

摘要翻译： 本发明涉及使用具有环境应激反应性和特定于给定组织中功能的新型启动子来特异性诱导给定组织中的基因表达。 通过以下方法实现：包括下述DNA（a），（b）或（c）的环境胁迫响应启动子下游具有任意基因的植物的步骤; （a）包含SEQ ID NO：1至8中任一个所示的核苷酸序列的DNA; （b）通过缺失，取代或添加一个或多个核苷酸并起到环境应激反应启动子的作用，其包含衍生自SEQ ID NO：1至8中任一项所示核苷酸序列的核苷酸序列的DNA; 或（c）在严格条件下与包含SEQ ID NO：1至8中任一所示的核苷酸序列的DNA杂交并用作环境胁迫响应启动子的DNA的DNA; 以及在存在环境胁迫的情况下培育植物的步骤，所述方法包括以组织特异性方式诱导位于环境胁迫响应启动子下游的基因的表达。

公开(公告)号：US07368630B2

公开(公告)日：2008-05-06

申请号：US11470154

申请日：2006-09-05

申请人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

发明人： Kazuo Shinozaki ,  Motoaki Seki ,  Miki Fujita

IPC分类号： C12N15/82 ,  A01H1/00

CPC分类号： C12N15/8238 ,  C07K14/415 ,  C12N15/8237 ,  C12N15/8273

摘要： The present invention provides a method of regulating expression of a gene by (a) preparing a recombinant plant cell line, plant tissue or plant with an expression vector having an abiotic environmental stress-responsive promoter of SEQ ID NO: 27 and the gene downstream thereof; and (b) culturing and cultivating the recombinant plant cell, plant tissue or plant under an abiotic environmental stress, wherein the promoter regulates the expression of the gene under the abiotic environmental stress.

公开(公告)号：US5323844A

公开(公告)日：1994-06-28

申请号：US998294

申请日：1992-12-30

申请人： Shigeto Sumitani ,  Takeshi Sato ,  Miki Fujita

发明人： Shigeto Sumitani ,  Takeshi Sato ,  Miki Fujita

IPC分类号： F24F3/06 ,  F25B13/00 ,  F25B41/04 ,  F25B29/00

CPC分类号： F24F3/06 ,  F25B13/00 ,  F25B41/04 ,  F25B2313/008 ,  F25B2313/009 ,  F25B2313/02341 ,  F25B2313/02343 ,  F25B2313/02344 ,  F25B2313/02541 ,  F25B2313/0316 ,  F25B2341/0652 ,  F25B2600/02 ,  F25B2600/2513 ,  F25B2700/2104 ,  Y02B30/72

摘要： A refrigerant heating type air conditioner operable in a cooling mode and a heating mode includes a first external heat exchanger which works as a condenser in the cooling mode and a second external heat-exchanger which works as an evaporator. Bypass means is disposed between a discharge side of a compressor and the second external heat-exchanger. An expansion valve is connected to an inner heat-exchanger, which works as a condenser in the heating mode, and to both the first and second external heat-exchangers. A control valve is disposed between a suction port side of the second external heat-exchanger for preventing the refrigerant from flowing to the second external heat-exchanger in the cooling mode. Pressure of the refrigerant which is trapped between the control valve and the expansion valve is increased by applying heat, by heating means, to the second external heat-exchanger. The heated refrigerant is released to the bypass means when its pressure exceeds to the pressure of discharged refrigerant from the compressor.

摘要翻译： 以冷却模式和加热模式可操作的制冷剂加热型空调器包括作为冷却模式的冷凝器的第一外部热交换器和作为蒸发器工作的第二外部热交换器。 旁通装置设置在压缩机的排出侧和第二外部热交换器之间。 膨胀阀连接到作为加热模式中的冷凝器的内部热交换器以及第一和第二外部热交换器两者。 控制阀设置在第二外部热交换器的吸入口侧之间，用于防止制冷剂在制冷模式下流向第二外部热交换器。 通过加热装置将热量加热到第二外部热交换器来增加被捕获在控制阀和膨胀阀之间的制冷剂的压力。 当其压力超过压缩机排出的制冷剂的压力时，加热的制冷剂被释放到旁路装置。