公开(公告)号：US07687454B2

公开(公告)日：2010-03-30

申请号：US10661471

申请日：2003-09-12

申请人： Robert E. W. Hancock ,  B. Brett Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Melissa Rosenberger ,  Jon-Paul Steven Powers

发明人： Robert E. W. Hancock ,  B. Brett Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Melissa Rosenberger ,  Jon-Paul Steven Powers

IPC分类号： A61K38/00 ,  C07K5/00 ,  A61K51/00

CPC分类号： C12Q1/6883 ,  A61K38/10 ,  A61K38/1709 ,  A61K38/193 ,  C07K7/08 ,  C07K14/4723 ,  C07K14/521 ,  C07K14/7158 ,  C12Q2600/158 ,  G01N33/5047 ,  G01N33/505 ,  A61K2300/00

摘要： A method of identifying a polynucleotide or pattern of polynucleotides regulated by one or more sepsis or inflammatory inducing agents and inhibited by a peptide is described. A method of identifying a pattern of polynucleotide expression for inhibition of an inflammatory or septic response. The method includes contacting cells with LPS, LTA, CpG DNA and/or intact microbe or microbial components in the presence or absence of a cationic peptide; detecting a pattern of polynucleotide expression for the cells in the presence and absence of the peptide, wherein the pattern in the presence of the peptide represents inhibition of an inflammatory or septic response. Also included are compounds and agents identified by the methods of the invention. In another aspect, the invention provides methods and compounds for enhancing innate immunity in a subject.

摘要翻译： 描述了鉴定由一种或多种脓毒症或炎性诱导剂调节并被肽抑制的多核苷酸的多核苷酸或图案的方法。 鉴定抑制炎性或败血症反应的多核苷酸表达模式的方法。 该方法包括在存在或不存在阳离子肽的情况下使细胞与LPS，LTA，CpG DNA和/或完整的微生物或微生物组分接触; 在存在和不存在肽的情况下检测细胞的多核苷酸表达的模式，其中在肽存在下的图案表示炎症或败血病应答的抑制。 还包括通过本发明的方法鉴定的化合物和试剂。 另一方面，本发明提供用于增强受试者的先天免疫力的方法和化合物。

公开(公告)号：US20070190533A1

公开(公告)日：2007-08-16

申请号：US11241882

申请日：2005-09-29

申请人： Robert Hancock ,  B. Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Rosenberger ,  Jon-Paul Powers

发明人： Robert Hancock ,  B. Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Rosenberger ,  Jon-Paul Powers

IPC分类号： C12Q1/68 ,  G01N33/567

CPC分类号： G01N33/564 ,  G01N2500/00 ,  G01N2800/26

摘要： The present invention provides a method of identifying agents that enhance innate immunity in a subject. The invention further provides a method of selectively supresses sepsis by suppressing expression of a proinflammatory gene while maintaining expression of an anti-inflammatory gene. Also provided are methods of identifying a polynucleotide or pattern of polynucleotides regulated by one or more sepsis or inflammatory inducing agents and inhibited by a peptide is described, methods of identifying a pattern of polynucleotide expression for inhibition of an inflammatory or septic response, and compounds and agents identified by the methods of the invention.

摘要翻译： 本发明提供一种识别增强受试者中先天免疫力的药剂的方法。 本发明还提供了一种通过抑制促炎基因的表达同时保持抗炎基因的表达来选择性抑制败血症的方法。 还提供了鉴定由一种或多种败血症或炎性诱导剂调节并被肽抑制的多核苷酸的多核苷酸或图案的方法，描述了鉴定抑制炎性或败血病应答的多核苷酸表达模式的方法，以及化合物和 通过本发明的方法鉴定的试剂。

公开(公告)号：US07390873B2

公开(公告)日：2008-06-24

申请号：US10403104

申请日：2003-03-28

申请人： Timothy J. Falla ,  Robert E. W. Hancock ,  Monisha Gough

发明人： Timothy J. Falla ,  Robert E. W. Hancock ,  Monisha Gough

IPC分类号： C07K17/00 ,  A61K38/00 ,  A61K39/00 ,  C12Q1/18

CPC分类号： C07K7/06 ,  A61K38/00 ,  C07K7/08 ,  C07K14/4723 ,  C07K2319/00 ,  Y02A50/475

摘要： A novel class of cationic peptides having antimicrobial activity is disclosed. These peptides can be encompassed by the formulas: X1X1PX2X3X2P(X2X2P)nX2X3(X5)0;(SEQ ID NO:23) X1X1PX2X3X4(X5)rPX2X3X3;(SEQ ID NO:24) X1X1X3(PW)uX3X2X5X2X2X5X2(X5)0; and(SEQ ID NO:25) X1X1X3X3X2P(X2X2P)nX2(X5)m;(SEQ ID NO:26) wherein: m is 1 to 5; n is 1 or 2; o is 2 to 5; r is 0 to 8; u is 0 or 1; X1 is Isoleucine, Leucine, Valine, Phenylalanine, Tyrosine, Tryptophan or Methionine; X2 represents Tryptophan or Phenylalanine X3 represents Arginine or Lysine; X4 represents Tryptophan or Lysine; and X5 represents Phenylalanine, Tryptophan, Arginine, Lysine, or Proline. The invention also provides a method of producing a cationic peptide variant having antimicrobial activity.

公开(公告)号：US20070134261A1

公开(公告)日：2007-06-14

申请号：US11400411

申请日：2006-04-07

申请人： Robert Hancock ,  B. Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Rosenberger ,  Jon-Paul Steven Powers ,  Jie Yu ,  Neeloffer Mookherjee

发明人： Robert Hancock ,  B. Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Rosenberger ,  Jon-Paul Steven Powers ,  Jie Yu ,  Neeloffer Mookherjee

IPC分类号： A61K39/02 ,  C12Q1/68

CPC分类号： A61K38/10 ,  A61K38/1709 ,  A61K38/193 ,  C07K7/08 ,  C07K14/4723 ,  C07K14/521 ,  C07K14/7158 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/136 ,  C12Q2600/158 ,  G01N33/5047 ,  G01N33/505 ,  G01N33/564 ,  G01N2500/00 ,  G01N2800/26 ,  A61K2300/00

摘要： The present invention provides a method of identifying agents that enhance innate immunity in a subject. The invention further provides a method of selectively supressing sepsis by suppressing expression of a proinflammatory gene while maintaining expression of an anti-inflammatory gene. Also provided are methods of identifying a polynucleotide or pattern of polynucleotides regulated by one or more sepsis or inflammatory inducing agents and inhibited by a peptide is described, methods of identifying a pattern of polynucleotide expression for inhibition of an inflammatory or septic response, and compounds and agents identified by the methods of the invention.

摘要翻译： 本发明提供一种识别增强受试者中先天免疫力的药剂的方法。 本发明还提供了通过抑制促炎基因的表达同时保持抗炎基因的表达来选择性抑制败血症的方法。 还提供了鉴定由一种或多种败血症或炎性诱导剂调节并被肽抑制的多核苷酸的多核苷酸或图案的方法，描述了鉴定抑制炎性或败血病应答的多核苷酸表达模式的方法，以及化合物和 通过本发明的方法鉴定的试剂。

公开(公告)号：US07507787B2

公开(公告)日：2009-03-24

申请号：US10308905

申请日：2002-12-02

申请人： Robert E. W. Hancock ,  B. Brett Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Melissa Rosenberger ,  Jon-Paul Steven Powers

发明人： Robert E. W. Hancock ,  B. Brett Finlay ,  Monisha Gough Scott ,  Dawn Bowdish ,  Carrie Melissa Rosenberger ,  Jon-Paul Steven Powers

IPC分类号： A61K38/00 ,  A61K38/28 ,  A61K38/04 ,  C07K14/00 ,  C07K16/00

CPC分类号： A61K38/1709 ,  A61K38/10 ,  A61K38/193 ,  C07K7/08 ,  C07K14/4723 ,  C07K14/521 ,  C07K14/7158 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N33/505 ,  A61K2300/00

摘要： A method of identifying a polynucleotide or pattern of polynucleotides regulated by one or more sepsis or inflammatory inducing agents and inhibited by a peptide is described. A method of identifying a pattern of polynucleotide expression for inhibition of an inflammatory or septic response. The method includes contacting cells with LPS, LTA, CpG DNA and/or intact microbe or microbial components in the presence or absence of a cationic peptide; detecting a pattern of polynucleotide expression for the cells in the presence and absence of the peptide, wherein the pattern in the presence of the peptide represents inhibition of an inflammatory or septic response. Also included are compounds and agents identified by the methods of the invention. In another aspect, the invention provides methods and compounds for enhancing innate immunity in a subject.

摘要翻译： 描述了鉴定由一种或多种脓毒症或炎性诱导剂调节并被肽抑制的多核苷酸的多核苷酸或图案的方法。 鉴定抑制炎性或败血症反应的多核苷酸表达模式的方法。 该方法包括在存在或不存在阳离子肽的情况下使细胞与LPS，LTA，CpG DNA和/或完整的微生物或微生物组分接触; 在存在和不存在肽的情况下检测细胞的多核苷酸表达的模式，其中在肽存在下的图案表示炎症或败血病应答的抑制。 还包括通过本发明的方法鉴定的化合物和试剂。 另一方面，本发明提供用于增强受试者的先天免疫力的方法和化合物。

公开(公告)号：US20080009445A1

公开(公告)日：2008-01-10

申请号：US11707813

申请日：2007-02-15

申请人： Robert Hancock ,  Monisha Gough ,  Aleksander Patrzykat ,  Donald Woods ,  Xiaoyan Jia

发明人： Robert Hancock ,  Monisha Gough ,  Aleksander Patrzykat ,  Donald Woods ,  Xiaoyan Jia

IPC分类号： A61K31/711 ,  A61K38/16 ,  A61P31/04

CPC分类号： A61K31/711 ,  A61K38/00 ,  C07K14/4723 ,  Y02A50/473 ,  Y02A50/481

摘要： A novel class of cationic peptides having antimicrobial activity is provided. Exemplary peptides of the invention include KWKSFIKKLTSAAKKVVTTAKPLALIS (SEQ ID NO:3) and KGWGSFFKKAAHVGKHVGKAALTHYL (SEQ ID NO:15). Also provided are methods for inhibiting the growth of bacteria utilizing the peptides of the invention. Such methods are useful for the treatment of respiratory infections, such as in cystic fibrosis patients. Such methods are further useful for accelerating wound healing.

摘要翻译： 提供了一类具有抗菌活性的新型阳离子肽。 本发明的示例性肽包括KWKSFIKKLTSAAKKVVTTAKPLALIS（SEQ ID NO：3）和KGWGSFFKKAAHVGKHVGKAALTHYL（SEQ ID NO：15）。 还提供了利用本发明的肽抑制细菌生长的方法。 这些方法可用于治疗呼吸道感染，如囊性纤维化患者。 这种方法对于加速伤口愈合更有用。

公开(公告)号：US06191254B1

公开(公告)日：2001-02-20

申请号：US08702054

申请日：1996-08-23

申请人： Timothy J. Falla ,  Robert E. W. Hancock ,  Monisha Gough

发明人： Timothy J. Falla ,  Robert E. W. Hancock ,  Monisha Gough

IPC分类号： A61K3800

CPC分类号： C07K7/06 ,  A61K38/00 ,  C07K7/08 ,  C07K14/4723 ,  C07K2319/00 ,  Y02A50/475

摘要： A novel class of cationic peptides having antimicrobial activity is disclosed. These peptides can be encompassed by the formulas: X1X1PX2X3X2P(X2X2P)nX2X3(X5)o; (SEQ ID NO: 23) X1X1PX2X3X4(X5)rPX2X3X3; (SEQ ID NO: 24) X1X1X3(PW)uX3X2X5X2X2X5X2(X5)o; and (SEQ ID NO: 25) X1X1X3X3X2P(X2X2P)nX2(X5)m; (SEQ ID NO: 26) wherein: m is 1 to 5; n is 1 or 2; o is 2 to 5; r is 0 to 8; u is 0 or 1; X1 is Isoleucine, Leucine, Valine, Phenylalanine, Tyrosine, Tryptophan or Methionine; X2 represents Tryptophan or Phenylalanine X3 represents Arginine or Lysine; X4 represents Tryptophan or Lysine; and X5 represents Phenylalanine, Tryptophan, Arginine, Lysine, or Proline. The invention also provides a method of producing a cationic peptide variant having antimicrobial activity.

摘要翻译： 公开了一类具有抗微生物活性的新型阳离子肽。 这些肽可以由下式涵盖：其中：m为1至5; n为1或2; o为2至5; r为0至8; u为0或1; X1为异亮氨酸，亮氨酸，缬氨酸，苯丙氨酸 ，酪氨酸，色氨酸或甲硫氨酸; X2表示色氨酸或苯丙氨酸，X 3表示精氨酸或赖氨酸; X 4表示色氨酸或赖氨酸; 并且X 5表示苯丙氨酸，色氨酸，精氨酸，赖氨酸或脯氨酸。本发明还提供了具有抗微生物活性的阳离子肽变体的制备方法。