公开(公告)号：US11001802B2

公开(公告)日：2021-05-11

申请号：US16120040

申请日：2018-08-31

Applicant: Nunc A/S

Inventor： Benjamin Fryer ,  Shelley Nelson ,  Villy Nielsen ,  Tina Kristensen Marwood ,  Thomas Brevig

IPC: C12N5/00 ,  C12N5/0735

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

公开(公告)号：US20190249138A1

公开(公告)日：2019-08-15

申请号：US16120040

申请日：2018-08-31

Applicant: Janssen Biotech, Inc. ,  Nunc A/S

Inventor： Benjamin Fryer ,  Shelley Nelson ,  Villy Nielsen ,  Tina Kristensen Marwood ,  Thomas Brevig

IPC: C12N5/0735

CPC classification number: C12N5/0606 ,  C12N2501/70 ,  C12N2509/00 ,  C12N2533/00

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

公开(公告)号：US20240376434A1

公开(公告)日：2024-11-14

申请号：US18690262

申请日：2022-09-16

Applicant: LIFE TECHNOLOGIES CORPORATION ,  NUNC A/S

Inventor： Debra JOHNSON ,  Sune SPORRING

IPC: C12N5/0775 ,  A61K35/28 ,  C12N9/22

Abstract: The instant technology generally relates to methods and compositions for expansion of mesenchymal stem cells in culture on a modified surface in the absence of a cell feeder layer and the absence of a cell adhesive coating on the surface. In some instances, the mesenchymal stem cells are expanded on the modified surface in a serum-free culture medium.