公开(公告)号：US20190249138A1

公开(公告)日：2019-08-15

申请号：US16120040

申请日：2018-08-31

Applicant: Janssen Biotech, Inc. ,  Nunc A/S

Inventor： Benjamin Fryer ,  Shelley Nelson ,  Villy Nielsen ,  Tina Kristensen Marwood ,  Thomas Brevig

IPC: C12N5/0735

CPC classification number: C12N5/0606 ,  C12N2501/70 ,  C12N2509/00 ,  C12N2533/00

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

公开(公告)号：US11001802B2

公开(公告)日：2021-05-11

申请号：US16120040

申请日：2018-08-31

Applicant: Nunc A/S

Inventor： Benjamin Fryer ,  Shelley Nelson ,  Villy Nielsen ,  Tina Kristensen Marwood ,  Thomas Brevig

IPC: C12N5/00 ,  C12N5/0735

Abstract: The present invention relates to the field of mammalian cell culture, and provides methods and compositions for cell attachment to, cultivation on and detachment from a solid substrate surface containing from at least about 0.5% N, a sum of O and N of greater than or equal to 17.2% and a contact angle of at least about 13.9 degrees, lacking a feeder cell layer and lacking an adlayer. In one embodiment of the present invention, the cells are treated with a compound capable of inhibiting Rho kinase activity. In another embodiment, the cells are treated with a compound capable of inhibiting Rho activity.

公开(公告)号：US10421948B2

公开(公告)日：2019-09-24

申请号：US15683594

申请日：2017-08-22

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania ,  Benjamin Fryer

IPC: C12N5/071

Abstract: The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.

公开(公告)号：US09951314B2

公开(公告)日：2018-04-24

申请号：US15274749

申请日：2016-09-23

Applicant: Janssen Biotech, Inc.

Inventor： Benjamin Fryer

IPC: C12N5/00 ,  C12N5/071

CPC classification number: C12N5/0678 ,  C12N2500/34 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/19 ,  C12N2501/727 ,  C12N2506/02 ,  C12N2533/54

Abstract: The present invention provides methods to promote the differentiation of pluripotent stem cells into insulin producing cells. In particular, the present invention provides a method to produce a population of cells, wherein greater than 80% of the cells in the population express markers characteristic of the definitive endoderm lineage.

公开(公告)号：US20170355963A1

公开(公告)日：2017-12-14

申请号：US15683594

申请日：2017-08-22

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania ,  Benjamin Fryer

IPC: C12N5/071

Abstract: The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.

公开(公告)号：US09752126B2

公开(公告)日：2017-09-05

申请号：US14963436

申请日：2015-12-09

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania ,  Benjamin Fryer

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/39 ,  C12N2501/405 ,  C12N2501/41 ,  C12N2501/70 ,  C12N2501/998 ,  C12N2506/02

Abstract: The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.

公开(公告)号：US20160160182A1

公开(公告)日：2016-06-09

申请号：US14963436

申请日：2015-12-09

Applicant: Janssen Biotech, Inc.

Inventor： Alireza Rezania ,  Benjamin Fryer

IPC: C12N5/071

CPC classification number: C12N5/0676 ,  C12N2501/115 ,  C12N2501/117 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/39 ,  C12N2501/405 ,  C12N2501/41 ,  C12N2501/70 ,  C12N2501/998 ,  C12N2506/02

Abstract: The present invention provides a method for increasing the expression of MAFA in cells expressing markers characteristic of the pancreatic endocrine lineage comprising the steps of culturing the cells expressing markers characteristic of the pancreatic endocrine lineage in medium comprising a sufficient amount of a cyclin-dependent kinase inhibitor to cause an increase in expression of MAFA.

Abstract translation: 本发明提供了增加表达胰腺内分泌谱系特征标志物的细胞中MAFA表达的方法，包括以下步骤：在含有足量的细胞周期蛋白依赖性激酶抑制剂的培养基中培养表达胰腺内分泌谱系特征性标志物的细胞 导致MAFA的表达增加。

公开(公告)号：US20240101963A1

公开(公告)日：2024-03-28

申请号：US18231208

申请日：2023-08-07

Applicant: President and Fellows of Harvard College ,  Janssen Biotech, Inc.

Inventor： Quinn P. Peterson ,  Felicia J. Pagliuca ,  Douglas A. Melton ,  Jeffrey Robert Millman ,  Michael Saris Segel ,  Mads Gurtler ,  Alireza Rezania ,  Benjamin Fryer

IPC: C12N5/071 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0735 ,  C12N5/074

CPC classification number: C12N5/0678 ,  A61K35/39 ,  A61P3/10 ,  A61P5/48 ,  A61P5/50 ,  C12N5/0606 ,  C12N5/0676 ,  C12N5/0696 ,  C12N2501/11 ,  C12N2501/117 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2501/375 ,  C12N2501/385 ,  C12N2501/395 ,  C12N2501/40 ,  C12N2501/41 ,  C12N2501/727 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/03 ,  C12N2506/22 ,  C12N2506/45

Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.

公开(公告)号：US10377989B2

公开(公告)日：2019-08-13

申请号：US13998974

申请日：2013-12-30

Applicant: Janssen Biotech, Inc.

Inventor： Benjamin Fryer ,  Daina Laniauskas ,  Marcia Blackmoore ,  Haiyun Wang ,  Kostadinka LiLova ,  Shelley Nelson ,  Elizabeth Rosocha

IPC: C12N5/071 ,  C12N5/0735

Abstract: The present invention provides methods of preparing aggregated pluripotent stem cell clusters for differentiation.

公开(公告)号：US20190169575A1

公开(公告)日：2019-06-06

申请号：US16273076

申请日：2019-02-11

Applicant: President and Fellows of Harvard College ,  Janssen Biotech, Inc.

Inventor： Quinn P. Peterson ,  Felicia J. Pagliuca ,  Douglas A. Melton ,  Jeffrey R. Millman ,  Michael Saris Segel ,  Mads Gurtler ,  Alireza Rezania ,  Benjamin Fryer

IPC: C12N5/071 ,  C12N5/074 ,  C12N5/0735

Abstract: Disclosed herein are methods, compositions, kits, and agents useful for inducing β cell maturation, and isolated populations of SC-β cells for use in various applications, such as cell therapy.