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    Methods for culturing mammalian taste cells
    2.
    发明申请
    Methods for culturing mammalian taste cells 失效

    公开(公告)号：US20060121534A1

    公开(公告)日：2006-06-08

    申请号：US11249854

    申请日：2005-10-13

    Applicant: Nancy Rawson ,  Mehmet Ozdener

    Inventor： Nancy Rawson ,  Mehmet Ozdener

    IPC: G01N33/53 ,  C12N5/08 ,  C12N15/87

    CPC classification number: C12N5/062 ,  C12N2501/33 ,  C12N2533/54

    Abstract: The invention provides methods of culturing mammalian taste cells, including taste receptor cells. Cells are maintained for a duration of up to three months and longer while maintaining molecular and functional characteristics of mature taste cells. The cells are cultured on coated cell culture vessels and, from first replacement of medium onwards, the medium is replaced in intervals of at least 5 days. The invention further provides isolation and culturing methods of taste cells wherein the time that the cells are exposed to isolation solution and proteolytic enzymes is minimized and the cells are cultured in coated culture vessels with the medium replaced in intervals of at least 5 days from first replacement onwards. The invention further provides cultured taste cells, transfection and assay methods, and taste cell assay buffers with an osmolarity of about 300-320 and pH of about 7.0-7.3.

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