公开(公告)号：US11958753B2

公开(公告)日：2024-04-16

申请号：US18191152

申请日：2023-03-28

申请人： ENERGYSOURCE MINERALS LLC

发明人： Charles R. Marston ,  Michael J. Garska

IPC分类号： C01D15/08 ,  B01D15/02 ,  B01D15/12 ,  B01D15/18 ,  C01D15/02

CPC分类号： C01D15/08 ,  B01D15/02 ,  B01D15/125 ,  B01D15/1807 ,  C01D15/02 ,  B01D2215/023

摘要： This invention relates generally to a process for selective adsorption and recovery of lithium from natural and synthetic brines, and more particular to a process for recovering lithium from a natural or synthetic brine solution by passing the brine solution through a lithium selective adsorbent in a continuous countercurrent adsorption and desorption circuit.

公开(公告)号：US11667671B2

公开(公告)日：2023-06-06

申请号：US16911511

申请日：2020-06-25

申请人： Cytiva BioProcess R&D AB

发明人： Annika Forss ,  Mats Ander ,  Tomas Bjorkman ,  Hans Blom ,  Jesper Hansson ,  Gustav Rodrigo

IPC分类号： C07K1/16 ,  C07K1/22 ,  C07K16/00 ,  B01J20/286 ,  B01J20/285 ,  B01D15/18 ,  B01D15/34 ,  C07K14/31

CPC分类号： C07K1/165 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/34 ,  B01J20/285 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K2317/21 ,  C07K2317/31 ,  C07K2319/30

摘要： The invention relates to a method of isolating an immunoglobulin, comprising the steps of:



a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml;
b) contacting a liquid sample comprising an immunoglobulin with the separation matrix;
c) washing the separation matrix with a washing liquid;
d) eluting the immunoglobulin from the separation matrix with an elution liquid; and
e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.

公开(公告)号：US20180353875A1

公开(公告)日：2018-12-13

申请号：US16060043

申请日：2016-12-12

申请人： BASF SE

发明人： Pedro Sa Gomes ,  Bastiaan Bram Pieter Staal ,  Felicitas Guth ,  Nigel A. Langley

IPC分类号： B01D15/34 ,  B01D15/18 ,  C08G65/30 ,  C08G65/08

CPC分类号： B01D15/34 ,  B01D15/1807 ,  B01D15/1821 ,  B01D15/1871 ,  B01D15/1892 ,  C08G65/08 ,  C08G65/30 ,  C08G2650/58

摘要： A process for purification of polyether block copolymers comprising polyoxyethylene and polyoxypropylene moieties using sequential multi-column size exclusion chromatography apparatus operated as a counter current moving bed wherein a process cycle comprises the steps of.

公开(公告)号：US20170361244A1

公开(公告)日：2017-12-21

申请号：US15533591

申请日：2015-12-15

申请人： GE HEALTHCARE BIO-SCIENCES AB

发明人： Karol Maciej Lacki

IPC分类号： B01D15/16 ,  B01D15/38 ,  B01D15/18 ,  G01N30/42 ,  G01N30/88

CPC分类号： B01D15/163 ,  B01D15/1807 ,  B01D15/1871 ,  B01D15/20 ,  B01D15/3809 ,  G01N30/42 ,  G01N30/56 ,  G01N30/88 ,  G01N2030/889

摘要： Certain embodiments of the invention provides a method for monitoring level of saturation of a chromatography media in a column, which method comprises measuring a first pressure at the inlet of an unloaded column; measuring a second pressure at the inlet from a loaded column; and comparing the first and second pressure measurement to determine the level of saturation of the chromatography media. Embodiments of the invention also provide related methods for controlling a chromatography system and methods for controlling a periodic counter current chromatography system, as well as a chromatography system suitable for use with the novel methods.

公开(公告)号：US20170241992A1

公开(公告)日：2017-08-24

申请号：US15593213

申请日：2017-05-11

申请人： ChromaCon AG

发明人： Thomas MULLER-SPATH ,  Lars AUMANN ,  Guido STROHLEIN ,  Michael BAVAND ,  Nicole ULMER

IPC分类号： G01N33/52 ,  B01D15/18 ,  B01D15/38

CPC分类号： G01N33/52 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/1871 ,  B01D15/3804 ,  B01D15/3809 ,  G01N30/468 ,  G01N30/8658

摘要： Provided is a method for control and/or monitoring and/or optimization of a chromatographic process, in which the method comprises at least 2 columns which are operated, alternatingly, wherein this operation can be carried out in that the at least 2 columns are operated in interconnected and disconnected states, wherein the columns switch positions after such a sequence of interconnected and disconnected state, and wherein downstream of at least one, or of each column, a detector is located capable of detecting the desired product and/or impurities when passing the detector.

公开(公告)号：US09718713B2

公开(公告)日：2017-08-01

申请号：US13768477

申请日：2013-02-15

申请人： Brown University

发明人： Joseph Calo ,  Madhavan Lakshmi ,  Johannes Kirchner ,  Euan Bain

IPC分类号： B01D15/00 ,  B01D15/02 ,  B01D24/00 ,  B01D29/00 ,  B01D21/01 ,  C02F1/52 ,  B03D3/00 ,  C02F1/68 ,  B01D15/20 ,  B01J20/02 ,  B01D15/22 ,  C02F1/28 ,  B01D24/32 ,  B01D24/46 ,  C02F1/00 ,  B01J19/18 ,  B01J8/00 ,  B01J10/00 ,  B01D37/00 ,  B01D15/08 ,  B01D15/18 ,  B01D15/26 ,  B01D24/36 ,  C02F1/70 ,  C02F101/10

CPC分类号： C02F1/68 ,  B01D15/08 ,  B01D15/1807 ,  B01D15/1892 ,  B01D15/206 ,  B01D15/22 ,  B01D15/26 ,  B01D24/36 ,  B01J20/0229 ,  C02F1/281 ,  C02F1/683 ,  C02F1/705 ,  C02F2101/103 ,  C02F2303/08

摘要： A system is provided for removing arsenic from water to safe levels at or below the EPA standards. The system is a hybrid spouted vessel/fixed bed filter system that significantly enhances/improves arsenic removal for drinking water using zero-valent iron (ZVI) particles. Movement of the circulating, iron-containing particles in a dense moving bed that forms on the spouted vessel bottom creates an abrasive “self-polishing” action among them that continuously generates colloidal iron corrosion products. This material then circulates with the water in the vessel and is removed and concentrated in a fixed bed filter. The colloidal material captured and immobilized in the filter has been shown to remove arsenic from contaminated water at very rapid rates.

公开(公告)号：US09650413B2

公开(公告)日：2017-05-16

申请号：US14627559

申请日：2015-02-20

申请人： Genzyme Corporation

发明人： Konstantin Konstantinov ,  Rahul Godawat ,  Veena Warikoo ,  Sujit Jain

IPC分类号： C07K1/22 ,  C07K16/00 ,  C12N9/00 ,  C12P21/00

CPC分类号： C07K1/16 ,  B01D15/1807 ,  B01D15/1864 ,  B01D15/1871 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01D15/3814 ,  B01D15/422 ,  C07K1/18 ,  C07K1/22 ,  C07K1/36 ,  C07K16/00 ,  C07K2317/14 ,  C12M23/52 ,  C12M47/10 ,  C12M47/12 ,  C12N9/00 ,  C12N9/2402 ,  C12N9/2465 ,  C12P21/00 ,  C12P21/005 ,  C12Y302/01022 ,  C12Y302/01045

摘要： Provided herein are integrated continuous biomanufacturing processes for producing a therapeutic protein drug substance. Also provided are systems that are capable of continuously producing a therapeutic protein drug substance.

公开(公告)号：US20160312168A1

公开(公告)日：2016-10-27

申请号：US15105505

申请日：2014-12-29

申请人： GE Healthcare Bio-Sciences Corp.

发明人： Vincent Francis PIZZI

IPC分类号： C12M1/42 ,  C12M1/00 ,  B01D15/02 ,  B01D21/01 ,  B01D15/12 ,  B01D63/02 ,  B01D61/14 ,  C12M1/26 ,  B01D15/18

CPC分类号： C12M35/04 ,  B01D15/02 ,  B01D15/125 ,  B01D15/1807 ,  B01D15/1821 ,  B01D21/01 ,  B01D61/145 ,  B01D61/147 ,  B01D63/02 ,  C07K1/22 ,  C07K1/34 ,  C12M23/14 ,  C12M23/26 ,  C12M33/14 ,  C12M47/02 ,  C12M47/10

摘要： The invention discloses an apparatus (1;11;31) for cell cultivation, comprising a bioreactor (2;12;32), an acoustic standing wave cell separator (5;15;35) and a filter (7;17;37), wherein an outlet (3;13;33) of the bioreactor is fluidically connected to an inlet (4;14;34) of the acoustic standing wave cell separator and a media outlet (6;16;36) of the acoustic standing wave cell separator is fluidically connected to the filter (7;17;37).

摘要翻译： 本发明公开了一种用于细胞培养的装置（1; 11; 31），包括生物反应器（2; 12; 32），声驻波隔离器（5; 15; 35）和过滤器（7; 17; 37） ，其中所述生物反应器的出口（3; 13; 33）流体连接到所述声驻波分离器的入口（4; 14; 34）和所述声驻波的介质出口（6; 16; 36） 电池隔板流体连接到过滤器（7; 17; 37）。

公开(公告)号：US20160271520A1

公开(公告)日：2016-09-22

申请号：US15074017

申请日：2016-03-18

申请人： BIOTECHFLOW LTD

发明人： Martin John HOFMANN

IPC分类号： B01D15/18 ,  B01D15/26 ,  C07K1/22 ,  B01D15/22

CPC分类号： B01D15/1807 ,  B01D15/22 ,  B01D15/265 ,  C07K1/22 ,  G01N30/56 ,  G01N30/6004 ,  G01N30/6017

摘要： A separation column for expanded bed adsorption comprises a column tube (2), a base (15) carrying an inlet rotor structure (6) for pumping in process liquid, and a top cap (3). The top cap (3) is conical in form, and has a peripheral flange 31 by which it is rigidly fixed to the top edge flange (22) of the column tube (2). The angle of the convergent interior surface (35) of the conical top cap (3) may be between 10 and 25°. A vortex-inhibitor component (8) projects down below the outlet structure (4) at the top of the cap, projecting into the operating space (15) of the column to inhibit rotation of liquid in the column interior. An expanded bed adsorption process is done with upward flow of liquid in the column through a bed of media particles.

摘要翻译： 用于膨胀床吸附的分离塔包括柱管（2），承载用于泵送处理液体的入口转子结构（6）的底座（15）和顶盖（3）。 顶盖（3）是圆锥形的，并具有周边凸缘31，刚性地固定在柱管（2）的顶边缘凸缘（22）上。 锥形顶盖（3）的会聚内表面（35）的角度可以在10°和25°之间。 涡流抑制剂组件（8）在盖的顶部向下突出到出口结构（4）的下方，突出到柱的操作空间（15）中，以阻止液体在柱内部的旋转。 膨胀床吸附过程通过柱中的液体向上流动通过介质颗粒床进行。

公开(公告)号：US09220997B2

公开(公告)日：2015-12-29

申请号：US12532806

申请日：2008-03-28

申请人： Allan Lihme ,  René Oehlenschläger Holte ,  Kurt Hauge Jensen ,  Tony Christensen

发明人： Allan Lihme ,  René Oehlenschläger Holte ,  Kurt Hauge Jensen ,  Tony Christensen

IPC分类号： B01D15/18 ,  C07K1/22 ,  G01N30/38

CPC分类号： B01D15/1807 ,  C07K1/22 ,  G01N30/38

摘要： In the field of expanded bed adsorption chromatography, with particular but not exclusive relevance to disposable expanded bed chromatography columns, a method of conducting upward flow expanded bed chromatography comprising: supplying a liquid via an inlet to a stationary phase medium contained in a column, allowing adsorption of at least one component from the liquid by the stationary phase medium, withdrawing the liquid from the column via an outlet, regulating the expansion of the stationary phase medium by regulation of the flow rate of the liquid through at least the inlet, and restricting any overpressure in the headspace of the column to not more than the outside pressure plus 0.1 bar, is provided. In addition, apparatus for use in said method, in particular columns for use in expanded bed chromatography, are provided.

摘要翻译： 在膨胀床吸附色谱领域中，与一次性膨胀床层析色谱柱特别但不排他地相关，一种进行向上流扩张床层析的方法，包括：通过入口将液体供给到容纳在柱中的固定相介质，允许 通过固定相介质从液体中吸收至少一种组分，通过出口从柱中排出液体，通过至少通过入口限制液体的流速来调节固定相介质的膨胀，并限制 提供柱的顶部空间中的任何超压力不超过外部压力加上0.1巴。 此外，提供了用于所述方法的设备，特别是用于扩展床层析的色谱柱。