摘要:
The present invention provides an improved method for the production of 1,3-propanediol from a variety of carbon sources in an organism capable of 1,3-propanediol production and comprising DNA encoding protein X of a microorganismal dehydratase or protein X in combination with at least one of protein 1, protein 2 and protein 3, which proteins are comparable to those encoded by orfY, orfX and orfW, respectively from a microorganismal dha regulon. The protein X may be isolated from a diol dehydratase or a glycerol dehydratase. The present invention also provides host cells comprising protein X that are capable of increased production of 1,3-propanediol.
摘要:
Recombinant organisms are provided comprising genes encoding cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates.
摘要:
A process of preparing a mushroom growth medium from a cellulosic material is provided in which the cellulosic material used in the solid-state fermentation process is chemically ammoniated. The chemical ammoniation step reduces composting time, particularly preconditioning time, while providing a compost and a mushroom bed material comparable to that achieved by commercial composting processes, which do not use ammoniated straw.
摘要:
A method of controlling toxic fungal diseases in mushrooms is provided which comprises adding an effective fungal-disease-inhibiting amount of a compound of the formula ##STR1## to a substrate in which mushrooms are growing or are to be grown, wherein R represents --CH.sub.2 OH or --CHO; n is an integer from 0 to 3; and each R.sup.1 independently represents halogen, OH, NH.sub.2, or an organic substituent containing from 1 to 10 carbon atoms and from 0 to 5 heteroatoms, wherein the total number of carbon and heteroatoms in all R.sup.1 substituents is no more than 15.
摘要:
Recombinant organisms are provided comprising genes encoding aquacobalamin reductase, cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates. More specifically the following nucleotide sequences are provided: btuR, encoding the E. coli cob(I)alamin adenosyltransferase enzyme; cobA, encoding the Salmonella typhimurium cob(I)alamin adenosyltransferase enzyme; cobO, encoding the Pseudomonas denitrificans cob(I)alamin adenosyltransferase enzyme; dhaB1, encoding the α subunit of the Klebsiella glycerol dehydratase enzyme; dhaB2, encoding the β subunit of the Klebsiella glycerol dehydratase enzyme; dhaB3, encoding the γ subunit of the Klebsiella glycerol dehydratase enzyme; dhaT, encoding Klebsiella oxidoreductase enzyme; the yciK gene isolated from E. coli; the glucose isomerase promoter sequence from Streptomyces; and the N-terminal amino acid sequence for cob(II)alamin reductase from Pseudomonas denitrificans.
摘要:
There are provided novel transposable elements isolated from Aspergillus. Also provided are novel fragments comprising the inverted repeat(s) of the transposable elements, such fragments being useful as probes to isolate transposable elements from other filamentous fungi.