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    PLANT HAVING REDUCED LIGNIN AND CELLULOSE CONTENTS WITHOUT REDUCING GLUCAN CONTENT, METHOD OF PRODUCING THE SAME AND UTILIZATION THEREOF
    5.
    发明申请
    PLANT HAVING REDUCED LIGNIN AND CELLULOSE CONTENTS WITHOUT REDUCING GLUCAN CONTENT, METHOD OF PRODUCING THE SAME AND UTILIZATION THEREOF 有权
    具有减少葡萄糖含量的减少木质素和纤维素含量的植物，其生产方法及其利用

    公开(公告)号：US20090019605A1

    公开(公告)日：2009-01-15

    申请号：US12280722

    申请日：2007-02-27

    申请人： Masaru Takagi ,  Nobutaka Mitsuda ,  Akira Iwase ,  Keiichiro Hiratsu

    发明人： Masaru Takagi ,  Nobutaka Mitsuda ,  Akira Iwase ,  Keiichiro Hiratsu

    IPC分类号： C12N15/11 ,  A01H5/00 ,  C12N15/82

    CPC分类号： C12N15/8246 ,  C12N15/8216 ,  C12N15/8255 ,  G01N33/5097 ,  G01N33/56961

    摘要： By inhibiting the function of a transcription factor that promotes transcription of a gene associated with the amounts of lignin and cellulose, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is produced. In this plant, glucan in the obtained cell wall components is in the state of highly easily undergoing saccharification. In this plant, moreover, natural dehiscence of pods is suppressed. A method of inhibiting the transcription factor includes a method in which a chimeric gene between a transcription factor gene and a polynucleotide that encodes a functional peptide capable of converting the transcription factor into a transcription repressor is introduced into a plant cell so that a chimeric protein in which the transcription factor is fused with the functional peptide is produced in a plant cell, and a method of inhibiting the expression of the transcription factor, such as knockout method or RNAi method. Thus, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is provided.

    摘要翻译： 通过抑制促进与木质素和纤维素的量相关的基因的转录的转录因子的功能，产生其中木素和纤维素的量减少而不减少葡聚糖的量的植物。 在该植物中，获得的细胞壁组分中的葡聚糖处于非常容易进行糖化的状态。 此外，在这种植物中，荚的自然开裂被抑制。 抑制转录因子的方法包括将转录因子基因和能够将转录因子转录成转录抑制子的功能性肽的多核苷酸之间的嵌合基因引入植物细胞中，使嵌合蛋白质 在植物细胞中产生转录因子与功能性肽融合的转录因子，以及抑制转录因子表达的方法，例如敲除方法或RNAi方法。 因此，提供了在不降低葡聚糖的量的情况下还原木质素和纤维素的量的植物。

    Plant having reduced lignin and cellulose contents without reducing glucan content, method of producing the same and utilization thereof
    8.
    发明授权
    Plant having reduced lignin and cellulose contents without reducing glucan content, method of producing the same and utilization thereof 有权
    具有降低木素和纤维素含量而不降低葡聚糖含量的植物，其生产方法及其利用

    公开(公告)号：US08183432B2

    公开(公告)日：2012-05-22

    申请号：US12280722

    申请日：2007-02-27

    申请人： Masaru Takagi ,  Nobutaka Mitsuda ,  Akira Iwase ,  Keiichiro Hiratsu

    发明人： Masaru Takagi ,  Nobutaka Mitsuda ,  Akira Iwase ,  Keiichiro Hiratsu

    IPC分类号： C12N15/82 ,  C12N15/10 ,  C12N15/00 ,  A01H5/00

    CPC分类号： C12N15/8246 ,  C12N15/8216 ,  C12N15/8255 ,  G01N33/5097 ,  G01N33/56961

    摘要： By inhibiting the function of a transcription factor that promotes transcription of a gene associated with the amounts of lignin and cellulose, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is produced. In this plant, glucan in the obtained cell wall components is in the state of highly easily undergoing saccharification. In this plant, moreover, natural dehiscence of pods is suppressed. A method of inhibiting the transcription factor includes a method in which a chimeric gene between a transcription factor gene and a polynucleotide that encodes a functional peptide capable of converting the transcription factor into a transcription repressor is introduced into a plant cell so that a chimeric protein in which the transcription factor is fused with the functional peptide is produced in a plant cell, and a method of inhibiting the expression of the transcription factor, such as knockout method or RNAi method. Thus, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is provided.

    摘要翻译： 通过抑制促进与木质素和纤维素的量相关的基因的转录的转录因子的功能，产生其中木素和纤维素的量减少而不减少葡聚糖的量的植物。 在该植物中，获得的细胞壁组分中的葡聚糖处于非常容易进行糖化的状态。 此外，在这种植物中，荚的自然开裂被抑制。 抑制转录因子的方法包括将转录因子基因和能够将转录因子转录成转录抑制子的功能性肽的多核苷酸之间的嵌合基因引入植物细胞中，使嵌合蛋白质 在植物细胞中产生转录因子与功能性肽融合的转录因子，以及抑制转录因子表达的方法，例如敲除方法或RNAi方法。 因此，提供了在不降低葡聚糖的量的情况下还原木质素和纤维素的量的植物。

    Producing process of sterile plants, plants obtained by the process, and use of the plants
    9.
    发明申请
    Producing process of sterile plants, plants obtained by the process, and use of the plants 审中-公开
    无菌植物的生产过程，通过该过程获得的植物和植物的使用

    公开(公告)号：US20110099664A1

    公开(公告)日：2011-04-28

    申请号：US10574470

    申请日：2005-01-07

    申请人： Masaru Takagi ,  Keiichiro Hiratsu ,  Nobutaka Mitsuda

    发明人： Masaru Takagi ,  Keiichiro Hiratsu ,  Nobutaka Mitsuda

    IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N5/04 ,  A01H5/10 ,  C12N15/63

    CPC分类号： C12N15/8289 ,  C12N15/829

    摘要： Transcription of a gene associated with formation of floral organs is suppressed to produce a sterile plant. A plant cell is transfected with a chimeric gene that includes (i) a coding gene of a transcription factor that promotes expression of a gene associated with formation of floral organs, and (ii) a polynucleotide that encodes a functional peptide that converts an arbitrary transcription factor into a transcription repressor, and a chimeric protein in which the transcription factor is fused with the functional peptide is expressed in the plant cell. The expression of the gene associated with formation of floral organs is dominantly suppressed by the chimeric protein, and as a result a male sterile plant is produced that cannot properly form pollen. The chimeric protein also suppresses expression of a gene associated with dehiscence of anther, and as a result a plant is produced in which dehiscence of anther is suppressed. Further, the chimeric protein suppresses expression of target genes of a transcription factor associated with formation of stamen and pistil, and as a result a double flowered plant is produced.

    摘要翻译： 抑制与花器官形成相关的基因的转录以产生无菌植物。 用嵌合基因转染植物细胞，该嵌合基因包括（i）促进与花器官形成有关的基因表达的转录因子的编码基因，和（ii）编码功能性肽的多核苷酸，其转换任意转录 因子转录入抑制子，并且其中转录因子与功能性肽融合的嵌合蛋白在植物细胞中表达。 与花器官形成相关的基因的表达主要被嵌合蛋白抑制，结果产生不能正确形成花粉的雄性不育植物。 嵌合蛋白还抑制与花药开裂有关的基因的表达，结果产生了抑制花药开裂的植物。 此外，嵌合蛋白质抑制与雄蕊和雌蕊形成相关的转录因子的靶基因的表达，结果产生双花植物。