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公开(公告)号：US06303759B1
公开(公告)日：2001-10-16
申请号：US09310850
申请日：1999-05-13
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
IPC分类号： C07K1702
CPC分类号： G01N33/531 , G01N33/532 , Y10S436/80
摘要： The present invention provides a highly sensitive dye-labeled and polymerized antibody that can detect a target substance even when the target substance has a low concentration. The dye-labeled and polymerized antibody of the present invention comprises a polymerized antibody, which has been polymerized with a polyfunctional reagent, and a cyanine dye for labeling the polymerized antibody represented by the formula (1) given below: where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a range of 1 to 4.
摘要翻译： 本发明提供即使在目标物质浓度低的情况下也能够检测目标物质的高灵敏度的染料标记和聚合抗体。 本发明的染料标记和聚合抗体包含已经与多官能试剂聚合的聚合抗体和用于标记由下式给出的聚合抗体的花青染料：其中R1和R2表示氢 或烷基，X表示卤素，M表示氢或碱金属，n表示1〜4的整数。
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公开(公告)号：US07790470B2
公开(公告)日：2010-09-07
申请号：US10588546
申请日：2005-03-23
CPC分类号： B01F5/0057 , B01F1/0022 , B01F5/0206 , B01F2215/0037 , G01N21/03 , G01N21/76 , G01N2035/00544 , Y10T436/25
摘要： A method capable of stirring a liquid sample and a reagent promptly and easily includes the following steps (A) and (B). In step (A), using a cell including: a liquid sample retaining section having a plurality of particles; and a liquid sample supply inlet, a liquid sample containing an analyte is supplied from the liquid sample supply inlet to the liquid sample retaining section. In step (B), the liquid sample and a specific binding substance capable of specifically binding to the analyte are stirred by the movement of the particles caused by the flow of the liquid sample in the cell resulting from the supply of the liquid sample, to obtain a liquid mixture. The electric charge of at least the surface of the particles and the electric charge of the specific binding substance have the same polarity in the liquid mixture.
摘要翻译： 能够迅速且容易地搅拌液体样品和试剂的方法包括以下步骤（A）和（B）。 在步骤（A）中，使用包括：具有多个颗粒的液体样品保留部分的细胞; 将液体试样供给口从液体试样供给口供给到液体试样保持部。 在步骤（B）中，液体样品和能够特异性结合分析物的特异性结合物质由于由液体样品供应而导致的液体样品流动引起的颗粒运动而被搅拌至 获得液体混合物。 至少颗粒表面的电荷和特定结合物质的电荷在液体混合物中具有相同的极性。
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公开(公告)号：US06300480B1
公开(公告)日：2001-10-09
申请号：US09310851
申请日：1999-05-13
IPC分类号： C07K1702
CPC分类号： G01N33/532 , G01N33/531 , Y10S436/80
摘要： The present invention provides a dye-labeled protein conjugate in which a protein conjugate is labeled with a large number of dye molecules. In the dye-labeled protein conjugate, a protein conjugate that includes a protein and an antibody bound thereto via a disulfide bond is labeled with a cyanine dye represented by the formula (1) given below. where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a rage of 1 to 4.
摘要翻译： 本发明提供染料标记的蛋白质缀合物，其中蛋白质缀合物用大量染料分子标记。 在染料标记的蛋白质缀合物中，包含蛋白质和通过二硫键结合的抗体的蛋白质缀合物用下述式（1）表示的花青染料标记。其中R1和R2表示氢或烷基 X表示卤素，M表示氢或碱金属，n表示1〜4的整数。
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公开(公告)号：US20070172961A1
公开(公告)日：2007-07-26
申请号：US10588546
申请日：2005-03-23
IPC分类号： G01N1/00
CPC分类号： B01F5/0057 , B01F1/0022 , B01F5/0206 , B01F2215/0037 , G01N21/03 , G01N21/76 , G01N2035/00544 , Y10T436/25
摘要： A method capable of stirring a liquid sample and a reagent promptly and easily includes the following steps (A) and (B). In step (A), using a cell including: a liquid sample retaining section having a plurality of particles; and a liquid sample supply inlet, a liquid sample containing an analyte is supplied from the liquid sample supply inlet to the liquid sample retaining section. In step (B), the liquid sample and a specific binding substance capable of specifically binding to the analyte are stirred by the movement of the particles caused by the flow of the liquid sample in the cell resulting from the supply of the liquid sample, to obtain a liquid mixture. The electric charge of at least the surface of the particles and the electric charge of the specific binding substance have the same polarity in the liquid mixture.
摘要翻译： 能够迅速且容易地搅拌液体样品和试剂的方法包括以下步骤（A）和（B）。 在步骤（A）中，使用包括：具有多个颗粒的液体样品保留部分的细胞; 将液体试样供给口从液体试样供给口供给到液体试样保持部。 在步骤（B）中，液体样品和能够特异性结合分析物的特异性结合物质由于由液体样品供应而导致的液体样品流动引起的颗粒运动而被搅拌至 获得液体混合物。 至少颗粒表面的电荷和特定结合物质的电荷在液体混合物中具有相同的极性。
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公开(公告)号：US06307029B1
公开(公告)日：2001-10-23
申请号：US09311147
申请日：1999-05-13
IPC分类号： C07K1702
CPC分类号： G01N33/532 , G01N33/531 , Y10S436/80
摘要： The present invention provides a dye-labeled protein conjugate in which a protein conjugate is labeled with a large number of dye molecules. In the dye-labeled protein conjugate, a protein conjugate that includes a protein and an antibody bound thereto via a disulfide bond is labeled with a cyanine dye represented by the formula (1) or the formula (2) given below: where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a range of 1 to 4.
摘要翻译： 本发明提供染料标记的蛋白质缀合物，其中蛋白质缀合物用大量染料分子标记。 在染料标记的蛋白质缀合物中，包含蛋白质和通过二硫键结合的抗体的蛋白质缀合物用下面给出的式（1）或式（2）表示的花青染料标记：其中R1和R2 表示氢或烷基，X表示卤素，M表示氢或碱金属，n表示1〜4的整数。
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公开(公告)号：US06194225B1
公开(公告)日：2001-02-27
申请号：US09154025
申请日：1998-09-16
申请人： Miwa Oka , Nobuyuki Shigetou , Jinsei Miyazaki
发明人： Miwa Oka , Nobuyuki Shigetou , Jinsei Miyazaki
IPC分类号： G01N33533
CPC分类号： G01N33/76 , G01N33/54386 , G01N33/558 , Y10S435/97 , Y10S436/81 , Y10S436/823
摘要： An immunochromatography-assisted device is disclosed which facilitates accurate and rapid detection of an antigen contained in a fluid sample and from which background coloring and blank coloring possibly causing a malfunction at detection are successfully eliminated. The immunochromatography-assisted device comprises a porous carrier having an additive-impregnated part between a sample introducing part and a determining part, the additive-impregnated part carrying at least one selected from the group consisting of a surfactant, a water-soluble ammonium salt and a pH buffer such that it dissolves in a sample introduced into the carrier in order to move with the movement of the sample through the carrier. The porous carrier also has a labelled part segmented into plural zones.
摘要翻译： 公开了免疫色谱辅助装置，其有助于准确和快速地检测流体样品中所含的抗原，并且可以从中检测出可能导致检测故障的背景着色和毛坯着色。 免疫色谱辅助装置包括多孔载体，其在样品引入部分和确定部分之间具有添加剂浸渍部分，所述添加剂浸渍部分携带选自表面活性剂，水溶性铵盐和 pH缓冲液，使得其溶解在引入载体中的样品中，以随着样品通过载体的移动而移动。 多孔载体还具有被分割成多个区域的标记部分。
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公开(公告)号：US06303758B1
公开(公告)日：2001-10-16
申请号：US09310849
申请日：1999-05-13
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
IPC分类号： C07K1702
CPC分类号： G01N33/531 , G01N33/532 , Y10S436/80
摘要： The present invention provides a highly sensitive dye-labeled and polymerized antibody that can detect a target substance even when the target substance has a low concentration. The dye-labeled and polymerized antibody of the present invention comprises a polymerized antibody, which has been polymerized with a polyfunctional reagent, and a cyanine dye for labeling the polymerized antibody represented by the formula (1) or the formula (2) given below: where R1 and R2 denote hydrogen or an alkyl group, X denotes a halogen, M denotes hydrogen or an alkali metal, and n represents an integer in a range of 1 to 4.
摘要翻译： 本发明提供即使在目标物质浓度低的情况下也能够检测目标物质的高灵敏度的染料标记和聚合抗体。 本发明的染料标记和聚合抗体包含已经与多官能试剂聚合的聚合抗体和用于标记由下式给出的式（1）或式（2）表示的聚合抗体的花青染料： 其中R1和R2表示氢或烷基，X表示卤素，M表示氢或碱金属，n表示1〜4的整数。
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8.发明授权公开(公告)号：US06303757B1
公开(公告)日：2001-10-16
申请号：US09305289
申请日：1999-05-05
申请人： Nobuyuki Shigetou , Jinsei Miyazaki
发明人： Nobuyuki Shigetou , Jinsei Miyazaki
IPC分类号： C07K1702
CPC分类号： G01N33/58 , G01N33/532 , G01N33/583 , Y10S436/80
摘要： A high-sensitive indirect polymerized and labelled antibody is disclosed. The antibody facilitates detection of a low concentration of antigen as an analyte in a sample solution. The indirect polymerized and labelled antibody of the present invention is prepared by polymerizing an antibody using a multi-functional reagent, binding the polymerized antibody with a protein via a disulfide bond of the antibody to form a polymerized antibody conjugate, and labelling the conjugate with a cyanine dye represented by the following formula: where R1 and R2 represent hydrogen or an alkyl group, X represents a halogen, M represents hydrogen or an alkali metal, and n represents an integer of 1 to 4.
摘要翻译： 公开了一种高灵敏度的间接聚合和标记的抗体。 抗体有助于检测低浓度的抗原作为样品溶液中的分析物。 本发明的间接聚合和标记的抗体是通过使用多功能试剂聚合抗体，通过抗体的二硫键将聚合的抗体与蛋白质结合形成聚合的抗体缀合物，并用偶联物标记缀合物来制备的 由下式表示的花青染料：其中R1和R2表示氢或烷基，X表示卤素，M表示氢或碱金属，n表示1〜4的整数。
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公开(公告)号：US5922618A
公开(公告)日：1999-07-13
申请号：US831204
申请日：1997-04-02
申请人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
发明人： Nobuyuki Shigetou , Jinsei Miyazaki , Mahito Hirai
IPC分类号： G01N33/532 , G01N33/58 , G01N33/549 , C07K16/00
CPC分类号： G01N33/583 , G01N33/532 , Y10S436/80 , Y10S524/90
摘要： The invention relates to a dye-labeled antibody conjugate comprising an antibody conjugate and a cyanine dye. The dye-labeled antibody conjugate is prepared by polymerizing an antibody such as mouse IgG in phosphate buffer using a polyfunctional reagent such as dithiobis(sulfosuccinimidylpropionate), and stirring the antibody and a cyanine dye represented by Formula I . The dye-labeled antibody conjugate is more sensitive to antigens than conventional dye-labeled antibodies because it has many reactive sites to antigens. ##STR1## wherein X represents a halogen, M represents a hydrogen or alkali metal, and n represents an integer of 1 to 4
摘要翻译： 本发明涉及包含抗体缀合物和花青染料的染料标记的抗体缀合物。 通过使用多官能试剂如二硫代双（丙磺酸磺基琥珀酰亚胺酯）在磷酸盐缓冲液中聚合抗体如小鼠IgG，并搅拌该抗体和由式I表示的花青染料，来制备染料标记的抗体缀合物。 染料标记的抗体缀合物比常规染料标记的抗体对抗原更敏感，因为它具有许多抗原反应性位点。 其中X表示卤素，M表示氢或碱金属，n表示1〜4的整数
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