Preparation of support matrices for immobilized enzymes
    2.
    发明授权
    Preparation of support matrices for immobilized enzymes 失效
    固定化酶支持基质的制备

    公开(公告)号:US4193910A

    公开(公告)日:1980-03-18

    申请号:US951948

    申请日:1978-10-16

    摘要: Support matrices for immobilized enzymes which comprise a combined organic-inorganic support may be prepared by treating a solid, porous, inorganic, water-insoluble support such as an inorganic porous metal oxide with a styrene monomer, polymerizing the styrene on the solid support at polymerizing conditions, nitrating the resultant polystyrene-solid support composite and thereafter reducing the nitrated polystyrene-solid support composite to form an aminopolystyrene-solid support matrix.

    摘要翻译: 包含组合的有机 - 无机载体的固定化酶的支持基质可以通过用苯乙烯单体处理固体,多孔,无机,水不溶性载体如无机多孔金属氧化物来制备,在苯乙烯聚合时将苯乙烯聚合在固体载体上 条件下,硝化所得的聚苯乙烯 - 固体支持物复合物,然后还原硝化聚苯乙烯 - 固体载体复合物以形成氨基聚苯乙烯 - 固体载体基质。

    Dye-labeled antibody conjugate and preparation method thereof
    10.
    发明授权
    Dye-labeled antibody conjugate and preparation method thereof 失效
    染料标记的抗体缀合物及其制备方法

    公开(公告)号:US5922618A

    公开(公告)日:1999-07-13

    申请号:US831204

    申请日:1997-04-02

    摘要: The invention relates to a dye-labeled antibody conjugate comprising an antibody conjugate and a cyanine dye. The dye-labeled antibody conjugate is prepared by polymerizing an antibody such as mouse IgG in phosphate buffer using a polyfunctional reagent such as dithiobis(sulfosuccinimidylpropionate), and stirring the antibody and a cyanine dye represented by Formula I . The dye-labeled antibody conjugate is more sensitive to antigens than conventional dye-labeled antibodies because it has many reactive sites to antigens. ##STR1## wherein X represents a halogen, M represents a hydrogen or alkali metal, and n represents an integer of 1 to 4

    摘要翻译: 本发明涉及包含抗体缀合物和花青染料的染料标记的抗体缀合物。 通过使用多官能试剂如二硫代双(丙磺酸磺基琥珀酰亚胺酯)在磷酸盐缓冲液中聚合抗体如小鼠IgG,并搅拌该抗体和由式I表示的花青染料,来制备染料标记的抗体缀合物。 染料标记的抗体缀合物比常规染料标记的抗体对抗原更敏感,因为它具有许多抗原反应性位点。 其中X表示卤素,M表示氢或碱金属,n表示1〜4的整数