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    Methods for enriching CD34.sup.+ human hematopoietic progenitor cells
    1.
    发明授权
    Methods for enriching CD34.sup.+ human hematopoietic progenitor cells 失效
    富含CD34 +人造血祖细胞的方法

    公开(公告)号：US5474687A

    公开(公告)日：1995-12-12

    申请号：US299469

    申请日：1994-08-31

    申请人： Peter Van Vlasselaer

    发明人： Peter Van Vlasselaer

    IPC分类号： B01J20/32 ,  B01L3/14 ,  C12N5/00 ,  C12N5/0789 ,  G01N33/49 ,  B01D33/15

    CPC分类号： B01L3/5021 ,  B01L3/5082 ,  C12N5/0093 ,  C12N5/0647 ,  A61M2202/0439

    摘要： The present invention relates to methods of enriching hematopoietic progenitor cells from body fluids. In particular, it relates to the use of a cell-trap centrifugation tube containing a gradient solution adjusted to a specific density to enrich for CD34.sup.+ cells from apheresed blood. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to undesired cell populations allowing the progenitor cells to be separated during centrifugation in a more convenient manner. The rapid progenitor cell enrichment method described herein has a wide range of applications, including but not limited to, donor cell preparation for bone marrow transplantation without the use of invasive procedures such as bone marrow aspiration.

    摘要翻译： 本发明涉及从体液中富集造血祖细胞的方法。 特别地，本发明涉及使用含有调节至特定浓度的梯度溶液的细胞捕集器离心管，以从被提取的血液中富集CD34 +细胞。 该管允许在离心后通过倾析收集所需的细胞群，以最小化细胞损失并使效率最大化。 另外，通过使用细胞型特异性结合剂（例如与载体颗粒连接的抗体和凝集素）赋予不想要的细胞群体的不同密度的细胞型特异性结合剂，可以进一步简化该方法，从而允许在离心过程中分离祖细胞 以更方便的方式。 本文描述的快速祖细胞富集方法具有广泛的应用，包括但不限于用于骨髓移植的供体细胞制备，而不使用侵入性程序如骨髓抽吸。

    Separation apparatus and method
    2.
    发明授权
    Separation apparatus and method 失效
    分离装置及方法

    公开(公告)号：US5663051A

    公开(公告)日：1997-09-02

    申请号：US570397

    申请日：1995-12-11

    申请人： Peter Van Vlasselaer

    发明人： Peter Van Vlasselaer

    IPC分类号： B01L3/14 ,  C12N5/00 ,  C12N5/06 ,  G01N33/49 ,  G01N33/574

    CPC分类号： G01N33/491 ,  B01L3/5021 ,  B01L3/5082 ,  C12N5/0093 ,  A61M2202/0439 ,  Y10S435/803 ,  Y10S436/824

    摘要： Disclosed is an apparatus designed to be used for enriching specific cell types from cell mixtures. The apparatus includes a centrifugable device that includes a constriction defining a lower region and a defined cell separation medium. The constriction prevents mixing between the upper and lower portions of the device. Also disclosed are methods that use precisely defined cell separation media to isolate specific cells from cell mixtures, including CD34.sup.+ hematopoietic progenitor cells from blood or bone marrow, nucleated fetal cells from maternal blood, specific tumor cells, dendritic cells, natural killer cells, and natural suppressor cells from various body fluids, and for enrichment or depletion of T cell lymphocytes. Also disclosed is a density adjusted cell separation technique used to augment the above apparatus and enrichment methods. The apparatus and enrichment methods are useful in various diagnostic and therapeutic regimens.

    摘要翻译： 公开了一种设计用于从细胞混合物中富集特定细胞类型的装置。 该装置包括可离心装置，其包括限定下部区域的收缩部和限定的细胞分离介质。 缩颈防止装置的上部和下部之间的混合。 还公开了使用精确限定的细胞分离培养基从细胞混合物分离特定细胞的方法，所述细胞混合物包括来自血液或骨髓的CD34 +造血祖细胞，来自母体血液的有核胎儿细胞，特异性肿瘤细胞，树突状细胞，天然杀伤细胞和天然 来自各种体液的抑制细胞，以及T细胞淋巴细胞的富集或消耗。 还公开了一种用于增加上述装置和浓缩方法的密度调节细胞分离技术。 该设备和富集方法在各种诊断和治疗方案中是有用的。

    Methods for enriching breast tumor cells
    5.
    发明授权
    Methods for enriching breast tumor cells 失效
    丰富乳腺肿瘤细胞的方法

    公开(公告)号：US5648223A

    公开(公告)日：1997-07-15

    申请号：US299465

    申请日：1994-08-31

    申请人： Peter Van Vlasselaer

    发明人： Peter Van Vlasselaer

    IPC分类号： B01L3/14 ,  C12N5/00 ,  C12N5/06 ,  G01N33/49 ,  G01N33/574 ,  B01L11/00

    CPC分类号： G01N33/491 ,  B01L3/5021 ,  B01L3/5082 ,  C12N5/0093 ,  A61M2202/0439 ,  Y10S435/803 ,  Y10S436/824

    摘要： The present invention relates to methods of enriching breast tumor cells from a patient's body fluids. In particular, it relates to the use of a cell-trap centrifugation tube containing a specific density gradient solution adjusted to a specific density to enrich for breast tumor cells from a cell mixture. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to the non-tumor or tumor cell populations allowing the breast tumor cells to be separated from the non-tumor cells in a more convenient manner. The rapid breast tumor cell enrichment method described herein has a wide range of applications, including but not limited to, detection of tumor cells by molecular and immunochemical means, and purging of tumor cells from an autologous bone marrow cell preparation prior to re-infusion.

    摘要翻译： 本发明涉及从患者体液中丰富乳腺肿瘤细胞的方法。 特别地，本发明涉及使用一种含有特定密度梯度溶液的细胞捕集式离心管，该浓度调节至特定密度以从细胞混合物中富集乳腺肿瘤细胞。 该管允许在离心后通过倾析收集所需的细胞群，以最小化细胞损失并使效率最大化。 此外，该方法可以通过密度调整的细胞分选进一步简化，其使用细胞类型特异性结合剂，例如与载体颗粒连接的抗体和凝集素以赋予非肿瘤或肿瘤细胞群体不同的密度，允许乳腺肿瘤 细胞以更方便的方式与非肿瘤细胞分离。 本文描述的快速乳腺肿瘤细胞富集方法具有广泛的应用，包括但不限于通过分子和免疫化学方法检测肿瘤细胞，以及在重新输注之前从自体骨髓细胞制剂中清除肿瘤细胞。

    THERAPEUTIC METHODS AND COMPOSITIONS
    7.
    发明申请
    THERAPEUTIC METHODS AND COMPOSITIONS 审中-公开
    治疗方法和组合物

    公开(公告)号：US20110076272A1

    公开(公告)日：2011-03-31

    申请号：US12860838

    申请日：2010-08-20

    申请人： Victoria Smith ,  Peter Van Vlasselaer

    发明人： Victoria Smith ,  Peter Van Vlasselaer

    IPC分类号： A61K39/395 ,  C12Q1/26 ,  C12Q1/68 ,  C12N5/00 ,  A61K31/7088 ,  A61K31/713 ,  A61P35/00

    CPC分类号： C07K16/40 ,  A61K2039/505 ,  C07K2317/73 ,  C12N15/1137 ,  C12N2310/14 ,  C12N2310/531

    摘要： Disclosed herein are methods for modulating the environment of a tumor, by inhibiting the activity of the extracellular enzyme lysyl oxidase-like 2 (LOXL2). The methods disclosed herein are effective in reducing tumor growth, reducing recruitment of cells to the tumor, reducing fibroblast activation, reducing desmoplasia, reducing vasculogenesis, reducing the number of TAFs, reducing growth factor production, inhibiting collagen deposition, and increasing necrosis and pyknosis in the tumor. Exemplary inhibitors of LOXL2 activity are antibodies and siRNAs.

    摘要翻译： 本文公开了通过抑制细胞外酶赖氨酰氧化酶样2（LOXL2）的活性来调节肿瘤的环境的方法。 本文公开的方法有效减少肿瘤生长，减少细胞向肿瘤的募集，减少成纤维细胞的活化，减少发育不全，减少血管发生，减少TAF的数量，减少生长因子的产生，抑制胶原沉积，增加坏死和固缩 肿瘤。 LOXL2活性的示例性抑制剂是抗体和siRNA。

    METHOD OF TREATING HEPATITIS VIRUS INFECTION WITH A MULTIPHASIC INTERFERON DELIVERY PROFILE
    8.
    发明申请
    METHOD OF TREATING HEPATITIS VIRUS INFECTION WITH A MULTIPHASIC INTERFERON DELIVERY PROFILE 审中-公开
    用多功能干扰素递送谱描述乙型肝炎病毒感染的方法

    公开(公告)号：US20090196853A1

    公开(公告)日：2009-08-06

    申请号：US12420573

    申请日：2009-04-08

    申请人： Gary VISOR ,  Peter Van Vlasselaer

    发明人： Gary VISOR ,  Peter Van Vlasselaer

    IPC分类号： A61K38/21 ,  A61P31/12

    CPC分类号： A61K38/212 ,  A61K38/217

    摘要： The present invention provides methods of treating hepatitis virus infection. The methods generally involve administering a composition comprising an antiviral agent in a dosing regimen that achieves a multiphasic serum concentration profile of the antiviral agent. The dosing regimen includes dosing events that are less frequent than with currently available hepatitis therapies. The multiphasic antiviral agent serum concentration profile that is achieved using the methods of the invention effects an initial rapid drop in viral titer, followed by a further decrease in viral titer over time, to achieve a sustained viral response.

    摘要翻译： 本发明提供治疗肝炎病毒感染的方法。 所述方法通常包括在实现抗病毒剂的多相血清浓度分布的给药方案中施用包含抗病毒剂的组合物。 给药方案包括比目前可用的肝炎疗法不太频繁的给药事件。 使用本发明的方法实现的多相抗病毒剂血清浓度分布影响病毒滴度的初始快速下降，随后随着时间的推移进一步降低病毒滴度，以达到持续的病毒反应。

    Cell separation composition
    9.
    发明授权
    Cell separation composition 失效
    细胞分离组成

    公开(公告)号：US5789148A

    公开(公告)日：1998-08-04

    申请号：US570120

    申请日：1995-12-11

    申请人： Peter Van Vlasselaer ,  Varghese Palathumpat

    发明人： Peter Van Vlasselaer ,  Varghese Palathumpat

    IPC分类号： B01J20/32 ,  B01L3/14 ,  C12N5/00 ,  C12N5/0789 ,  G01N33/49

    CPC分类号： B01J20/261 ,  B01J20/103 ,  B01J20/3204 ,  B01J20/3244 ,  B01J20/327 ,  C12N5/0093 ,  C12N5/0647 ,  G01N33/491 ,  A61M2202/0439 ,  B01L2300/12 ,  B01L3/5021 ,  Y10S435/803 ,  Y10T428/2995

    摘要： Disclosed are a kit, composition and method for cell separation. The kit includes a centrifugable container and an organosilanized silica particle-based cell separation suspension suitable for density gradient separation, containing a polylactam and sterilized by treatment with ionizing radiation. The composition includes a silanized silica particle-based suspension for cell separation which contains at least 0.05% of a polylactam, and preferably treated by ionizing radiation. Also disclosed is a method of isolating rare blood cells from a blood cell mixture.

    摘要翻译： 公开了用于细胞分离的试剂盒，组合物和方法。 该试剂盒包括可离心容器和适用于密度梯度分离的有机硅烷化二氧化硅颗粒基细胞分离悬浮液，其含有聚内酰胺并通过用电离辐射处理灭菌。 该组合物包括用于细胞分离的硅烷化二氧化硅颗粒基悬浮液，其含有至少0.05％的聚内酰胺，并且优选通过电离辐射处理。 还公开了从血细胞混合物中分离稀有血细胞的方法。

    Methods for enriching fetal cells from maternal body fluids
    10.
    发明授权
    Methods for enriching fetal cells from maternal body fluids 失效
    从母体体液中丰富胎儿细胞的方法

    公开(公告)号：US5646004A

    公开(公告)日：1997-07-08

    申请号：US299468

    申请日：1994-08-31

    申请人： Peter Van Vlasselaer

    发明人： Peter Van Vlasselaer

    IPC分类号： B01L3/14 ,  C12N5/00 ,  C12N5/06 ,  G01N33/49 ,  G01N33/555 ,  B01L11/00

    CPC分类号： G01N33/491 ,  B01L3/5021 ,  B01L3/5082 ,  C12N5/0093 ,  A61M2202/0439 ,  Y10S435/803 ,  Y10S436/824

    摘要： The present invention relates to methods of enriching fetal cells from maternal body fluids. In particular, it relates to the use of a cell-trap centrifugation tube containing a gradient solution adjusted to a specific density to enrich for fetal nucleated red blood cells from maternal blood. The tube allows the desired cell population to be collected by decantation after centrifugation to minimize cell loss and maximize efficiency. In addition, the method can be further simplified by density-adjusted cell sorting which uses cell type-specific binding agents such as antibodies and lectins linked to carrier particles to impart a different density to undesired cell populations allowing the fetal cells to be separated during centrifugation in a more convenient manner. The rapid fetal cell enrichment method described herein has a wide range of applications, including but not limited to, gender determination and prenatal diagnosis of genetic diseases without the use of invasive procedures.

    摘要翻译： 本发明涉及从母体体液中富集胎儿细胞的方法。 特别地，本发明涉及使用含有调节至特定密度的梯度溶液以从母体血液中富集胎儿有核红细胞的细胞捕集器离心管。 该管允许在离心后通过倾析收集所需的细胞群，以最小化细胞损失并使效率最大化。 此外，该方法可以通过密度调整的细胞分选进一步简化，其使用细胞型特异性结合剂，例如与载体颗粒连接的抗体和凝集素，以赋予不想要的细胞群体不同的密度，从而允许在离心过程中分离胎儿细胞 以更方便的方式。 本文描述的快速胎儿细胞富集方法具有广泛的应用，包括但不限于性别测定和遗传疾病的产前诊断，而不使用侵入性程序。