公开(公告)号：US20070082002A1

公开(公告)日：2007-04-12

申请号：US11456511

申请日：2006-07-10

申请人： Peter Brams ,  Phillip Morrow

发明人： Peter Brams ,  Phillip Morrow

IPC分类号： A61K39/42 ,  C07H21/04 ,  C12P21/06 ,  C12N5/06 ,  C07K16/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/1027 ,  C07K2317/21 ,  C07K2317/92 ,  C12N2760/18522 ,  C12N2799/028

摘要： A highly efficient method for generating human antibodies in particular which are specific to be RSV fusion protein which combines in vitro primary of human spleen cells and antigen boosting in SCID mice is taught. This method provides for very high human antibody titers which are predominantly of the IgG isotype which contain antibodies of high specificity and affinity to desired antigens. This method is well suited for generating human monoclonal antibodies for therapeutic and diagnostic applications as well as for rescue of human cells for generation of combinational human antibody gene libraries. Two human monoclonal antibodies, RF-1 and RF-2 which each possess an affinity for RSV F-protein ≦2×10−9 Molar are taught as well as their corresponding amino acid and DNA sequences. These antibodies are to be used therapeutically and prophylactically for treating or preventing RSV infection, as well as for diagnosis of RSV in analytes.

摘要翻译： 教导了特异性产生特异性的人抗体的高效方法，其特征在于将体外原代人脾细胞和SCID小鼠中的抗原增强相结合的RSV融合蛋白。 该方法提供非常高的人抗体滴度，其主要是IgG同种型，其含有对所需抗原具有高特异性和亲和力的抗体。 该方法非常适用于产生用于治疗和诊断应用的人单克隆抗体以及用于拯救人细胞以产生组合人抗体基因文库。 每个对RSV F蛋白具有亲和力的两个人单克隆抗体RF-1和RF-2被教导以及它们相应的氨基酸和DNA序列。 这些抗体应用于治疗和预防性用于治疗或预防RSV感染以及分析物中RSV的诊断。

公开(公告)号：US20050281830A1

公开(公告)日：2005-12-22

申请号：US11041471

申请日：2005-01-24

申请人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

发明人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

IPC分类号： A61K39/40 ,  G01N33/554 ,  G01N33/569

CPC分类号： G01N33/6854 ,  A61K39/395 ,  A61K45/06 ,  A61K2039/505 ,  A61K2039/507 ,  C07K16/1278 ,  C07K2317/21 ,  G01N2333/35 ,  A61K2300/00

摘要： A highly efficient method for generating human antibodies using recall technology is provided. In one aspect, human antibodies which are specific to the anthrax toxin are provided. In one aspect, human peripheral blood cells that have been pre-exposed to anthrax toxin are used in the SCID mouse model. This method results in high human antibody titers which are primarily of the IgG isotype and which contain antibodies of high specificity and affinity to desired antigens. The antibodies generated by this method can be used therapeutically and prophylactically for preventing or treating mammals exposed to anthrax. Thus, in one embodiment, a prophylactic or therapeutic agent used to counter the effects of anthrax toxin, released as a mechanism of bioterrorism, is provided. In one embodiment, a formulation and method for preventing and/or treating anthrax infection comprising a binding agent that prevents the assembly of the PA63 heptamer is also provided. Methods for diagnosis and methods to determine anthrax contamination are also described.

摘要翻译： 提供了使用召回技术产生人抗体的高效方法。 一方面，提供了对炭疽毒素特异性的人抗体。 一方面，在SCID小鼠模型中使用预先暴露于炭疽毒素的人外周血细胞。 该方法导致高的人抗体滴度，其主要是IgG同种型，并且其含有对所需抗原具有高特异性和亲和力的抗体。 通过该方法产生的抗体可以治疗和预防用于预防或治疗暴露于炭疽的哺乳动物。 因此，在一个实施方案中，提供了用作抵抗作为生物恐怖机制的机制释放的炭疽毒素的作用的预防或治疗剂。 在一个实施方案中，还提供了用于预防和/或治疗炭疽感染的制剂和方法，其包含防止PA63七聚体组装的结合剂。 还描述了诊断方法和确定炭疽污染的方法。

公开(公告)号：US20110309044A1

公开(公告)日：2011-12-22

申请号：US13165540

申请日：2011-06-21

申请人： Michael Phillip Morrow

发明人： Michael Phillip Morrow

IPC分类号： A47F1/04 ,  G09F3/00 ,  G09F3/18 ,  A47B96/04

CPC分类号： A47F5/0068 ,  A47F5/005

摘要： The invention is embodied in an shelving system especially useful for carton flow warehouse shelving systems. The new system provides for carton flow lanes by mounting a series of shelf slotters along the replenish side shelf rail. Goods, typically in boxes, are placed into slots or lanes on the shelf platform, as guided by the shelf slotters. The series of parallel rollers allows the goods to travel down the shelf platform. Once the goods are directed into a lane by the shelf slotters, they will travel under the force of gravity down the roller tracks without significant deviation in their path, eliminating the need for a lane divider and reducing the labor involved in re-profiling a carton flow shelving system.

摘要翻译： 本发明体现在对于纸箱流动仓库货架系统特别有用的货架系统中。 新系统通过沿着补充侧搁架轨道安装一系列货架开槽器来提供纸箱流道。 商品，通常在箱子中，放置在货架平台上的狭槽或车道中，由货架开槽器引导。 一系列平行辊允许货物沿架子平台行进。 货物一旦通过货架开槽机被引导到车道中，它们将在重力的作用下沿着滚筒轨道行进，而不会在其路径上显着偏离，消除了对车道分隔器的需要，并减少了对纸箱进行重新分配的劳动 流动货架系统。

公开(公告)号：US20060246079A1

公开(公告)日：2006-11-02

申请号：US11040580

申请日：2005-01-21

申请人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

发明人： Phillip Morrow ,  Jeanne Morrow ,  Angray Kang ,  Fei Wang ,  Ivy Jiang ,  Ritsuko Sawada ,  Wolfgang Scholz

IPC分类号： A61K39/40 ,  C07K16/12

CPC分类号： C07K16/1278 ,  A61K39/40 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/56 ,  C07K2317/76 ,  C07K2317/92 ,  A61K2300/00

摘要： A highly efficient method for generating human antibodies using recall technology is provided. In one aspect, human antibodies which are specific to the anthrax toxin are provided. In one aspect, human peripheral blood cells that have been pre-exposed to anthrax toxin are used in the SCID mouse model. This method results in high human antibody titers which are primarily of the IgG isotype and which contain antibodies of high specificity and affinity to desired antigens. The antibodies generated by this method can be used therapeutically and prophylactically for preventing or treating mammals exposed to anthrax. Thus, in one embodiment, a prophylactic or therapeutic agent used to counter the effects of anthrax toxin, released as a mechanism of bioterrorism, is provided. In one embodiment, a formulation and method for preventing and/or treating anthrax infection comprising a binding agent that prevents the assembly of the PA63 heptamer is also provided. Methods for diagnosis and methods to determine anthrax contamination are also described.

摘要翻译： 提供了使用召回技术产生人抗体的高效方法。 一方面，提供了对炭疽毒素特异性的人抗体。 一方面，在SCID小鼠模型中使用预先暴露于炭疽毒素的人外周血细胞。 该方法导致高的人抗体滴度，其主要是IgG同种型，并且其含有对所需抗原具有高特异性和亲和力的抗体。 通过该方法产生的抗体可以治疗和预防用于预防或治疗暴露于炭疽的哺乳动物。 因此，在一个实施方案中，提供了用作抵抗作为生物恐怖机制的机制释放的炭疽毒素的作用的预防或治疗剂。 在一个实施方案中，还提供了用于预防和/或治疗炭疽感染的制剂和方法，其包含防止PA63七聚体组装的结合剂。 还描述了诊断方法和确定炭疽污染的方法。