-
1.发明授权公开(公告)号：US09505004B2
公开(公告)日：2016-11-29
申请号：US14383905
申请日：2012-03-09
申请人： Chen Su , Ping-Hua Teng , Chien-Chung Jeng
发明人： Chen Su , Ping-Hua Teng , Chien-Chung Jeng
CPC分类号： B01L7/525 , B01L2300/185 , B01L2400/0445 , B01L2400/082 , C12M41/12 , C12Q1/686
摘要： The present disclosure is related to a device for controlling thermal convection velocity of a biochemical reaction. The thermal convection velocity controlling device includes a base body for disposing a tube which is movable, wherein the tube is filled with a buffer of the biochemical reaction; a heating source located at a bottom of the tube or at a side of the tube to heat the buffer; and a flow rate adjusting apparatus for controlling a thermal convection flow direction of the buffer in the tube, whereby the flow rate adjusting apparatus changes a flow velocity and a flow time of the buffer. The present disclosure is also related to a method for controlling thermal convection velocity of a biochemical reaction using the device.
摘要翻译： 本公开涉及用于控制生化反应的热对流速度的装置。 热对流速度控制装置包括用于设置可移动的管的基体，其中管填充有生化反应的缓冲液; 位于所述管的底部或所述管的一侧以加热所述缓冲器的加热源; 以及用于控制管中的缓冲器的热对流流动方向的流量调节装置，由此流量调节装置改变缓冲器的流速和流动时间。 本公开还涉及使用该装置控制生化反应的热对流速度的方法。
-
公开(公告)号：US20130023010A1
公开(公告)日：2013-01-24
申请号：US13377490
申请日：2011-07-21
申请人： Ping Hua Teng , Cheng Su , Sih Ying Wu , Fu Chun Li
发明人： Ping Hua Teng , Cheng Su , Sih Ying Wu , Fu Chun Li
IPC分类号： C12P19/34
CPC分类号： C12N9/1276
摘要： A method for reverse transcription polymerase chain reaction comprises steps: preparing a capillary, and adding a reverse transcription enzyme into the capillary; and performing a lyophilization process on the RT enzyme contained by the capillary to fabricate the RT enzyme into a lyophilized RT reagent in the capillary. Therefore, a buffer solution, an RNA sample, a polymerase and a primer solution can be added into the capillary to re-dissolve the lyophilized RT reagent and enable a reverse transcription reaction and a polymerase chain reaction of the RNA sample to directly take place inside the capillary, so as to promote convenience and efficiency of experiment.
摘要翻译： 逆转录聚合酶链反应的方法包括以下步骤：制备毛细管，并向毛细管中加入逆转录酶; 并对由毛细管包含的RT酶进行冻干处理以将RT酶制成毛细管中的冻干的RT试剂。 因此，可以将缓冲溶液，RNA样品，聚合酶和引物溶液加入到毛细管中以重新溶解冻干的RT试剂，并使RNA样品的逆转录反应和聚合酶链反应直接发生在内部 毛细管，从而提高实验的便利性和效率。
-
公开(公告)号：US08574516B2
公开(公告)日：2013-11-05
申请号：US13324383
申请日：2011-12-13
申请人： Ping-Hua Teng , Cheng Su
发明人： Ping-Hua Teng , Cheng Su
摘要： An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat sink and a tube rack. The heat sink has a main body with a through hole for insertion of the test tube. The through hole has a relatively big diameter section and a relatively small diameter section located below the relatively big diameter section. The tube rack is mounted on the heat sink for insertion of the test tube. The apparatus can ensure that the temperature at the liquid level of the reaction mixture is lower than the temperature suitable for conducting the primer annealing step in the polymerase chain reaction process.
摘要翻译： 用于保持绝缘等温聚合酶链式反应的试管的装置包括散热器和管架。 散热器具有主体，其具有用于插入试管的通孔。 通孔具有相对较大的直径部分和位于较大直径部分下方的相对小的直径部分。 管架安装在散热器上，用于插入试管。 该装置可以确保反应混合物的液面温度低于聚合酶链反应过程中适合进行引物退火步骤的温度。
-
公开(公告)号：US20120244047A1
公开(公告)日：2012-09-27
申请号：US13323121
申请日：2011-12-12
申请人： Ping-Hua TENG , Cheng SU
发明人： Ping-Hua TENG , Cheng SU
IPC分类号： B01L9/06
摘要： An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat insulating mount and a heating member. The heat insulating mount has a main body provided with a receiving space for receiving a bottom of the test tube, a lateral channel communicated between the receiving space and an ambient environment, and an upper channel communicated between the receiving space and the ambient environment for insertion of the test tube. The heating member is inserted into the lateral channel for stopping at the bottom of the test tube. The apparatus can minimize the influence caused by the high temperature generated from the heat source on the heat dissipation of the reaction mixture in middle and upper sections of the test tube. The apparatus is suitable for the fluorescent detection of PCR reaction.
摘要翻译： 用于保持绝缘等温聚合酶链式反应的试管的装置包括隔热安装件和加热构件。 隔热安装座具有主体，该主体设置有用于接收试管的底部的接收空间，在接收空间与周围环境之间通信的横向通道，以及在接收空间与周围环境之间连通的上部通道用于插入 的试管。 将加热构件插入到横向通道中以停止在试管的底部。 该设备可以最小化由热源产生的高温对试管中部和上部的反应混合物的散热造成的影响。 该装置适用于PCR反应的荧光检测。
-
公开(公告)号：US20130146786A1
公开(公告)日：2013-06-13
申请号：US13315658
申请日：2011-12-09
申请人： Ping-Hua TENG , Cheng SU
发明人： Ping-Hua TENG , Cheng SU
IPC分类号： G01N21/64
CPC分类号： G01N21/645 , G01N2021/6471 , G01N2021/6473
摘要： An apparatus for generating fluorescence comprises a blue LED emitting a light beam, a filter and a fluorescent material. The filter is arranged in front of the light beam emitted by the blue LED, receives the light beam, and converts the light beam into a filtered light beam having wavelengths of 465-505 nm. The fluorescent material is arranged in one side of the filter, which is opposite to the blue LED, to receive the filtered light beam. The fluorescent material is excited by the filtered light beam to emit fluorescence. The filter is applied to control the wavelength and spectral range of the filtered light beam to achieve the best fluorescence exciting efficiency and prevent from the overlap of the wavelength ranges of the filtered light beam and the fluorescence.
摘要翻译： 用于产生荧光的装置包括发射光束的蓝色LED，滤光器和荧光材料。 滤光器布置在由蓝色LED发射的光束的前面，接收光束，并将光束转换成波长为465-505nm的滤波光束。 荧光材料布置在与蓝色LED相对的过滤器的一侧中，以接收经滤光的光束。 荧光材料被过滤的光束激发以发射荧光。 滤波器被应用于控制滤波光束的波长和光谱范围，以获得最佳的荧光激发效率，并防止滤波光束和荧光的波长范围的重叠。
-
公开(公告)号：US08409532B2
公开(公告)日：2013-04-02
申请号：US13323121
申请日：2011-12-12
申请人： Ping-Hua Teng , Cheng Su
发明人： Ping-Hua Teng , Cheng Su
摘要： An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat insulating mount and a heating member. The heat insulating mount has a main body provided with a receiving space for receiving a bottom of the test tube, a lateral channel communicated between the receiving space and an ambient environment, and an upper channel communicated between the receiving space and the ambient environment for insertion of the test tube. The heating member is inserted into the lateral channel for stopping at the bottom of the test tube. The apparatus can minimize the influence caused by the high temperature generated from the heat source on the heat dissipation of the reaction mixture in middle and upper sections of the test tube. The apparatus is suitable for the fluorescent detection of PCR reaction.
摘要翻译： 用于保持绝缘等温聚合酶链式反应的试管的装置包括隔热安装件和加热构件。 隔热安装座具有主体，该主体设置有用于接收试管的底部的接收空间，在接收空间与周围环境之间通信的横向通道，以及在接收空间和周围环境之间连通以便插入的上部通道 的试管。 将加热构件插入到横向通道中以停止在试管的底部。 该设备可以最小化由热源产生的高温对试管中部和上部的反应混合物的散热造成的影响。 该装置适用于PCR反应的荧光检测。
-
7.发明申请公开(公告)号：US20130071917A1
公开(公告)日：2013-03-21
申请号：US13234655
申请日：2011-09-16
申请人： Chen Su , Ping-Hua Teng , Chien-Chung Jeng
发明人： Chen Su , Ping-Hua Teng , Chien-Chung Jeng
IPC分类号： C12M1/40
CPC分类号： B01L3/5082 , B01L3/50851 , B01L7/525 , B01L2300/0832 , B01L2300/1805 , B01L2400/0445
摘要： A capillary for apparatus of insulated isothermal polymerase chain reaction is mounted onto a test tube holder. The capillary includes a tube which has an elongated tubular housing space that is cut to form an axial cross section with a distance between left and right edges greater than the distance between front and rear edges. When reactants are filled in the elongated tubular housing space and heated to generate convection, the left and right edges of the axial cross section form a narrower passage to reduce flowing speed of heat flow in the convection and increase cycling duration of the convection. Hence reaction duration of the reactants is prolonged, and reaction efficiency of the apparatus of insulated isothermal polymerase chain reaction also is increased.
摘要翻译： 绝缘等温聚合酶链式反应装置的毛细管安装在试管支架上。 毛细管包括管，其具有细长的管状容纳空间，其被切割以形成轴向横截面，其左右边缘之间的距离大于前后边缘之间的距离。 当反应物填充在细长管状容纳空间中并加热以产生对流时，轴向横截面的左边缘和右边缘形成较窄的通道，以减小对流中热流的流动速度并增加对流的循环持续时间。 因此反应物的反应持续时间延长，绝热等温聚合酶链反应装置的反应效率也提高。
-
公开(公告)号：US20120244048A1
公开(公告)日：2012-09-27
申请号：US13324383
申请日：2011-12-13
申请人： Ping-Hua Teng , Cheng Su
发明人： Ping-Hua Teng , Cheng Su
IPC分类号： B01L9/06
摘要： An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat sink and a tube rack. The heat sink has a main body with a through hole for insertion of the test tube. The through hole has a relatively big diameter section and a relatively small diameter section located below the relatively big diameter section. The tube rack is mounted on the heat sink for insertion of the test tube. The apparatus can ensure that the temperature at the liquid level of the reaction mixture is lower than the temperature suitable for conducting the primer annealing step in the polymerase chain reaction process.
摘要翻译： 用于保持绝缘等温聚合酶链式反应的试管的装置包括散热器和管架。 散热器具有主体，其具有用于插入试管的通孔。 通孔具有相对较大的直径部分和位于较大直径部分下方的相对小的直径部分。 管架安装在散热器上，用于插入试管。 该装置可以确保反应混合物的液面温度低于聚合酶链反应过程中适合进行引物退火步骤的温度。
-
公开(公告)号：US20120094373A1
公开(公告)日：2012-04-19
申请号：US13013831
申请日：2011-01-26
申请人： Chen SU , Ping-Hua TENG
发明人： Chen SU , Ping-Hua TENG
IPC分类号： C12M1/40
CPC分类号： B01L3/5082 , B01L7/52 , B01L9/065 , B01L2300/16 , B01L2300/1805
摘要： A container for nucleic acid amplification reaction is disclosed. The container includes a capillary and a conductor. The conductor tightly surrounds the capillary for heating the capillary evenly.
摘要翻译： 公开了用于核酸扩增反应的容器。 容器包括毛细管和导体。 导体紧紧围绕毛细管以均匀地加热毛细管。
-
-
-
-
-
-
-
-
搜索结果
9 条记录 (耗时 0.016 秒)
