公开(公告)号：US20080067056A1

公开(公告)日：2008-03-20

申请号：US11804964

申请日：2007-05-21

Applicant: Peter Searson ,  Nirveek Bhatacharjee ,  Prashant Mali

Inventor： Peter Searson ,  Nirveek Bhatacharjee ,  Prashant Mali

IPC: B01J19/08

CPC classification number: A61N1/0428 ,  A61K9/0009 ,  A61N1/0436 ,  A61N1/0476 ,  A61N1/0496 ,  B01J2219/00454 ,  B01J2219/00626 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01L3/5027 ,  B01L2200/16 ,  B01L2300/0896 ,  B01L2400/0415 ,  B82Y5/00 ,  B82Y30/00

Abstract: A method for the controlled release of an agent (e.g., a biomolecule or nanoparticle) into a specified environment, includes the steps of: (a) providing an electrode or array of electrodes, (b) functionalizing the electrode's surface by introducing to it a molecule or molecules (e.g., thiols on a gold electrode) that chemically bond on the electrode surface and form themselves into a self-assembled monolayer (c) attaching or linking said agent to the molecules through a chemical (e.g., using a coupling group such as amine) or electrostatic (e.g., when the agent is DNA) linkage, and (d) electrochemically releasing the agent from the electrode surface.

Abstract translation: 将试剂（例如，生物分子或纳米颗粒）控制释放到特定环境中的方法包括以下步骤：（a）提供电极或电极阵列，（b）通过向其引入电极的表面进行功能化 分子或分子（例如，金电极上的硫醇），其在电极表面上化学键合并形成自组装单层（c）通过化学物质（例如使用偶联基团）连接或连接所述试剂与分子 作为胺）或静电（例如，当试剂为DNA时）连接，和（d）从电极表面电化学释放试剂。

公开(公告)号：US20200340012A1

公开(公告)日：2020-10-29

申请号：US16325679

申请日：2017-08-18

Applicant: Prashant Mali ,  Dhruva Katrekar ,  Ana Moreno Collado ,  The Regents of the University of California

Inventor： Prashant Mali ,  Dhruva Katrekar ,  Ana Moreno Collado

IPC: C12N15/86 ,  C12N15/113 ,  C12N9/22 ,  C12N7/00 ,  A61K35/76 ,  A61P25/04 ,  A61P33/06

Abstract: The present disclosure relates to a novel delivery system with unique modular CRISPR-Cas9 architecture that allows better delivery, specificity and selectivity of gene editing. It represents significant improvement over previously described split-Cas9 systems. The modular architecture is “regulatable”. Additional aspects relate to systems that can be both spatially and temporally controlled, resulting in the potential for inducible editing. Further aspects relate to a modified viral capsid allowing conjugation to homing agents.