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    (R)-2-hydroxy-3-phenylpropionate (d-phenyllactate) dehydrogenase and gene encoding the same
    1.
    发明授权
    (R)-2-hydroxy-3-phenylpropionate (d-phenyllactate) dehydrogenase and gene encoding the same 失效
    （R）-2-羟基-3-苯基丙酸酯（d-苯基乳酸）脱氢酶及其编码基因

    公开(公告)号：US06916641B2

    公开(公告)日：2005-07-12

    申请号：US10258472

    申请日：2001-04-26

    Applicant: Koichi Miyamoto ,  Naomi Sumida ,  Naoki Midoh ,  Takeshi Murakami ,  Rainer Zocher ,  Horst Kleinkauf

    Inventor： Koichi Miyamoto ,  Naomi Sumida ,  Naoki Midoh ,  Takeshi Murakami ,  Rainer Zocher ,  Horst Kleinkauf

    IPC: C12N1/21 ,  C12N9/02 ,  C12P7/42 ,  C12N9/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04

    CPC classification number: C12N9/0008 ,  C12P7/42

    Abstract: An objective of the present invention is to provide a D-phenyllactic acid dehydrogenase. Another objective of the present invention is to provide a gene which encodes the D-phenyllactic acid dehydrogenase. A D-phenyllactic acid dehydrogenase according to the present invention is a protein comprising an amino acid sequence of the amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that has one or more modifications selected from a substitution, a deletion, an addition and an insertion and has D-phenyllactic acid dehydrogenase activity. A D-phenyllactic acid dehydrogenase gene according to the present invention comprises a nucleotide sequence which encodes the D-phenyllactic acid dehydrogenase, for example the nucleotide sequence of SEQ ID NO: 1.

    Abstract translation: 本发明的目的是提供D-苯基乳酸脱氢酶。 本发明的另一个目的是提供编码D-苯基乳酸脱氢酶的基因。 根据本发明的D-苯基乳酸脱氢酶是包含SEQ ID NO：2的氨基酸序列的氨基酸序列或SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列的蛋白质，其具有一个 或更多选自取代，缺失，添加和插入的修饰，并具有D-苯基乳酸脱氢酶活性。 根据本发明的D-苯基乳酸脱氢酶基因包含编码D-苯基乳酸脱氢酶的核苷酸序列，例如SEQ ID NO：1的核苷酸序列。

    Method for the production of baccatin-III by enzymatic synthesis
    2.
    发明授权
    Method for the production of baccatin-III by enzymatic synthesis 失效
    通过酶合成制备浆果赤霉素Ⅲ的方法

    公开(公告)号：US06458568B1

    公开(公告)日：2002-10-01

    申请号：US09311084

    申请日：1999-05-13

    Applicant: Konrad Glund ,  Matthias Hoffmann ,  Wolfram Weckwerth ,  Rainer Zocher

    Inventor： Konrad Glund ,  Matthias Hoffmann ,  Wolfram Weckwerth ,  Rainer Zocher

    IPC: C12P1702

    CPC classification number: C12P17/02

    Abstract: A method for the continuous synthesis of baccatin-III is performed by means of the enzymatic acetylations of 10-deacetyl baccatin-III (10-DAB) in an enzyme reactor. This objective is accomplished by a method, for which an aqueous reaction batch is used, which contains a partially purified or a very pure acetyl transferase from a Taxus species and synthesis components, especially 10-DAB, acetyl compounds or optionally an acetyl coenzyme A, which can be regenerated, a protective colloid and optionally a buffer substance in solution. The solution is separated by a semipermeable membrane from an organic solvent, which serves as extraction agent for baccatin-III, the reaction batch being regenerated at regular intervals in an aqueous buffer system and the solvent continuously being exchanged. By conducting the reaction, the product can be removed continuously from the reaction batch for the partial synthesis of taxol and taxoter. By the above method, achievable yields are increased significantly.

    Abstract translation: 通过酶反应器中10-脱乙酰浆果赤霉素III（10-DAB）的酶促乙酰化进行浆果赤霉素III的连续合成方法。 该目的通过一种使用含水反应批料的方法来实现，该方法含有来自紫杉属物种和合成成分，特别是10-DAB，乙酰化合物或任选的乙酰辅酶A的部分纯化或非常纯的乙酰转移酶， 其可以再生，保护胶体和任选的溶液中的缓冲物质。 将溶液由半透膜与有机溶剂分离，其用作浆果赤霉素III的提取剂，反应批次在水性缓冲体系中以规则的间隔再生，溶剂连续交换。 通过进行反应，可以从反应批料中连续除去产物以部分合成紫杉醇和紫杉醇。 通过上述方法，可实现的产率显着增加。

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