公开(公告)号：US06916641B2

公开(公告)日：2005-07-12

申请号：US10258472

申请日：2001-04-26

申请人： Koichi Miyamoto ,  Naomi Sumida ,  Naoki Midoh ,  Takeshi Murakami ,  Rainer Zocher ,  Horst Kleinkauf

发明人： Koichi Miyamoto ,  Naomi Sumida ,  Naoki Midoh ,  Takeshi Murakami ,  Rainer Zocher ,  Horst Kleinkauf

IPC分类号： C12N1/21 ,  C12N9/02 ,  C12P7/42 ,  C12N9/04 ,  C07H21/04 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04

CPC分类号： C12N9/0008 ,  C12P7/42

摘要： An objective of the present invention is to provide a D-phenyllactic acid dehydrogenase. Another objective of the present invention is to provide a gene which encodes the D-phenyllactic acid dehydrogenase. A D-phenyllactic acid dehydrogenase according to the present invention is a protein comprising an amino acid sequence of the amino acid sequence of SEQ ID NO: 2 or a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that has one or more modifications selected from a substitution, a deletion, an addition and an insertion and has D-phenyllactic acid dehydrogenase activity. A D-phenyllactic acid dehydrogenase gene according to the present invention comprises a nucleotide sequence which encodes the D-phenyllactic acid dehydrogenase, for example the nucleotide sequence of SEQ ID NO: 1.

摘要翻译： 本发明的目的是提供D-苯基乳酸脱氢酶。 本发明的另一个目的是提供编码D-苯基乳酸脱氢酶的基因。 根据本发明的D-苯基乳酸脱氢酶是包含SEQ ID NO：2的氨基酸序列的氨基酸序列或SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列的蛋白质，其具有一个 或更多选自取代，缺失，添加和插入的修饰，并具有D-苯基乳酸脱氢酶活性。 根据本发明的D-苯基乳酸脱氢酶基因包含编码D-苯基乳酸脱氢酶的核苷酸序列，例如SEQ ID NO：1的核苷酸序列。

公开(公告)号：US20060121577A1

公开(公告)日：2006-06-08

申请号：US11330363

申请日：2006-01-12

申请人： Naoki Midoh ,  Shigeru Hoshiko ,  Takeshi Murakami

发明人： Naoki Midoh ,  Shigeru Hoshiko ,  Takeshi Murakami

IPC分类号： C07H17/08 ,  C07H21/04 ,  C12P19/62 ,  C12N15/74 ,  C12N1/21

CPC分类号： C12N15/52 ,  C12P19/62

摘要： The present invention provides an isolated polypeptide comprising a nucleotide sequence encoding a protein which is involved in midecamycin biosynthesis, wherein the protein contains an amino acid sequence selected from SEQ ID NOs: 2 to 10, 13, 14, 16, 19, 20, 22 to 26, and 28 to 38 or a modified amino acid sequence of the amino acid sequence having one or more amino acid modifications without affecting activity of the protein.

摘要翻译： 本发明提供了一种分离的多肽，其包含编码参与麦迪霉素生物合成的蛋白质的核苷酸序列，其中所述蛋白质含有选自SEQ ID NO：2至10,13,14,16,19,20,22的氨基酸序列 至26和28至38或具有一个或多个氨基酸修饰的氨基酸序列的修饰的氨基酸序列，而不影响蛋白质的活性。

公开(公告)号：US07795001B2

公开(公告)日：2010-09-14

申请号：US11330363

申请日：2006-01-12

申请人： Naoki Midoh ,  Shigeru Hoshiko ,  Takeshi Murakami

发明人： Naoki Midoh ,  Shigeru Hoshiko ,  Takeshi Murakami

IPC分类号： C12N1/21 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N15/52 ,  C12P19/62

摘要： The present invention provides an isolated polypeptide comprising a nucleotide sequence encoding a protein which is involved in midecamycin biosynthesis, wherein the protein contains an amino acid sequence selected from SEQ ID NOs: 2 to 10, 13, 14, 16, 19, 20, 22 to 26, and 28 to 38 or a modified amino acid sequence of the amino acid sequence having one or more amino acid modifications without affecting activity of the protein.

摘要翻译： 本发明提供了一种分离的多肽，其包含编码参与麦迪霉素生物合成的蛋白质的核苷酸序列，其中所述蛋白质含有选自SEQ ID NO：2至10,13,14,16,19,20,22的氨基酸序列 至26和28至38或具有一个或多个氨基酸修饰的氨基酸序列的修饰的氨基酸序列，而不影响蛋白质的活性。

公开(公告)号：US07285404B1

公开(公告)日：2007-10-23

申请号：US10070387

申请日：2000-09-07

申请人： Naoki Midoh ,  Kaoru Okakura ,  Koichi Miyamoto ,  Manabu Watanabe ,  Koji Yanai ,  Tetsuya Yasutake ,  Sato Aihara ,  Takafumi Futamura ,  Horst Kleinkauf ,  Takeshi Murakami

发明人： Naoki Midoh ,  Kaoru Okakura ,  Koichi Miyamoto ,  Manabu Watanabe ,  Koji Yanai ,  Tetsuya Yasutake ,  Sato Aihara ,  Takafumi Futamura ,  Horst Kleinkauf ,  Takeshi Murakami

IPC分类号： C12N9/00

CPC分类号： C12N9/00 ,  C12P21/00

摘要： The present invention provides an enzyme that synthesizes a cyclic depsipeptide, particularly the substance 1022, and a gene thereof. A cyclic depsipeptide synthetase according to the present invention comprises (a) an amino acid sequence of SEQ ID NO: 2 or (b) a modified amino acid sequence of the amino acid sequence of SEQ ID NO: 2 that have one or more modifications selected from a substitution, a deletion, an addition and an insertion and has cyclic depsipeptide synthetase activity. A cyclic depsipeptide synthetase gene according to the present invention comprises a nucleotide sequence encoding a cyclic depsipeptide synthetase. The present invention also provides a recombinant vector and a transformant for expressing the cyclic depsipeptide synthetase, and a mass production system for the cyclic depsipeptide. The present invention further provides a method for producing the cyclic depsipeptide synthetase.

摘要翻译： 本发明提供合成环状缩肽，特别是物质1022及其基因的酶。 根据本发明的循环倍肽合成酶包含（a）SEQ ID NO：2的氨基酸序列或（b）SEQ ID NO：2的氨基酸序列的修饰的氨基酸序列，其具有一个或多个修饰选择 从取代，缺失，添加和插入，并具有循环衰老肽合成酶活性。 根据本发明的环状缩肽酶合成酶基因包含编码环状倍肽合成酶的核苷酸序列。 本发明还提供了用于表达环状缩酚酸肽合成酶的重组载体和转化体，以及用于环状缩酚酸肽的批量生产系统。 本发明还提供了一种用于制备环状缩酚酸肽合成酶的方法。

公开(公告)号：US07109018B1

公开(公告)日：2006-09-19

申请号：US10089514

申请日：2000-09-29

申请人： Koji Yanai ,  Kaoru Okakura ,  Shohei Yasuda ,  Manabu Watanabe ,  Koichi Miyamoto ,  Naoki Midoh ,  Takeshi Murakami

发明人： Koji Yanai ,  Kaoru Okakura ,  Shohei Yasuda ,  Manabu Watanabe ,  Koichi Miyamoto ,  Naoki Midoh ,  Takeshi Murakami

IPC分类号： C12N1/20 ,  C12N5/10 ,  C07H21/04

CPC分类号： C12N9/0004 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P13/001 ,  C12P13/02

摘要： An objective of the present invention is to provide a transformant altered so as to produce a secondary metabolite in which a benzene ring of the secondary product is modified at the para-position with a functional group containing a nitrogen atom. A transformant according to the present invention is a transformant of an organism producing a secondary metabolite having a benzene ring skeleton without substitution at the para-position with a functional group containing a nitrogen atom, said transformant being transformed by introducing genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a gene encoding an amino acid sequence (SEQ ID NO: 2) having 4-amino-4-deoxychorismic acid synthase activity, a gene encoding an amino acid sequence (SEQ ID NO: 4) having 4-amino-4-deoxychorismic acid mutase activity and a gene encoding an amino acid sequence (SEQ ID NO: 6) having 4-amino-4-deoxyprephenic acid dehydrogenase activity, so as to produce a secondary metabolite having a benzene ring skeleton substituted at the para-position with a functional group containing a nitrogen atom. Another objective of the present invention is to provide a novel gene involved in the biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid. A novel gene according to the present invention comprises genes encoding the amino acid sequences of SEQ ID NOs: 2, 4 and 6 or modified sequences thereof.

摘要翻译： 本发明的目的是提供改性的转化体，以便产生次级代谢物，其中次级产物的苯环在对位上用含氮原子的官能团改性。 根据本发明的转化体是产生具有苯环骨架的次级代谢物的生物体的转化体，其在具有含氮原子的官能团的对位没有取代，所述转化体通过引入参与生物合成途径的基因而转化 包括编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的编码氨基酸序列（SEQ ID NO：4）的氨基酸序列（SEQ ID NO：2）的基因的氨基苯基丙酮酸， 具有4-氨基-4-脱氧酸性变位酶活性的基因，以及编码具有4-氨基-4-脱氧蝶呤酸脱氢酶活性的氨基酸序列（SEQ ID NO：6）的基因，以产生具有苯环骨架的次级代谢物 在对位上用含有氮原子的官能团取代。 本发明的另一个目的是提供一种涉及从氯酸到对氨基苯基丙酮酸生物合成途径的新基因。 根据本发明的新基因包含编码SEQ ID NO：2,4和6的氨基酸序列的基因或其修饰序列。

公开(公告)号：US07070980B2

公开(公告)日：2006-07-04

申请号：US10229148

申请日：2002-08-28

申请人： Naoki Midoh ,  Shigeru Hoshiko ,  Takeshi Murakami

发明人： Naoki Midoh ,  Shigeru Hoshiko ,  Takeshi Murakami

IPC分类号： C12N1/21 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N15/52 ,  C12P19/62

摘要： The present invention provides an isolated polypeptide comprising a nucleotide sequence encoding a protein which is involved in midecamycin biosynthesis, wherein the protein contains an amino acid sequence selected from SEQ ID NOs: 2 to 10, 13, 14, 16, 19, 20, 22 to 26, and 28 to 38 or a modified amino acid sequence of the amino acid sequence having one or more amino acid modifications without affecting activity of the protein.

摘要翻译： 本发明提供了一种分离的多肽，其包含编码参与麦迪霉素生物合成的蛋白质的核苷酸序列，其中所述蛋白质含有选自SEQ ID NO：2至10,13,14,16,19,20,22的氨基酸序列 至26和28至38或具有一个或多个氨基酸修饰的氨基酸序列的修饰的氨基酸序列，而不影响蛋白质的活性。