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公开(公告)号：US20050090002A1
公开(公告)日：2005-04-28
申请号：US10614191
申请日：2003-07-08
Applicant: Ranieri Cancedda , Beatrice Dozin
Inventor： Ranieri Cancedda , Beatrice Dozin
CPC classification number: C12N5/0655 , A61K35/12 , C12N5/0663 , C12N2500/25 , C12N2500/36 , C12N2500/38 , C12N2500/90 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/125 , C12N2501/135 , C12N2501/235 , C12N2501/39
Abstract: The present invention provides serum free media for growth and proliferation of chondrocytes and mesenchymal stem cells in cultures.
Abstract translation: 本发明提供用于培养物中软骨细胞和间充质干细胞生长和增殖的无血清培养基。
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2.发明授权
公开(公告)号：US5670308A
公开(公告)日：1997-09-23
申请号：US446821
申请日：1995-06-01
Applicant: Giuseppe Geraci , Mario De Rosa , Mose Rossi , Ranieri Cancedda , Michele De Luca , Graziella Pellegrini
Inventor： Giuseppe Geraci , Mario De Rosa , Mose Rossi , Ranieri Cancedda , Michele De Luca , Graziella Pellegrini
CPC classification number: A01N1/0221 , A01N1/02
Abstract: A method for the preservation of in vitro expanded epithelial sheets by incubation of the sheets in a cryopreserving solution characterized by lack of volume increase at the liquid-solid phase transition. The cryopreserving solutions comprise: a) polyethyleneglycols having molecular weight between 3 and 5 KD; and b) at least one cross-linking agent selected from polyols, polyamines, mono- or oligosaccharides, and polyethyleneglycols having molecular weight lower than 1 KD.
Abstract translation: PCT No.PCT / EP93 / 03364 371日期：1995年6月1日 102（e）1995年6月1日PCT 1993年12月1日PCT PCT。 出版物WO94 / 13135 日期1994年6月23日一种通过在冷冻保存溶液中温育片材来保存体外扩增的上皮片的方法，其特征在于在液 - 固相转变时体积增加不足。 冷冻保存溶液包括：a）分子量在3和5KD之间的聚乙二醇; 和b）选自分子量低于1KD的多元醇，多胺，单糖或寡糖和聚乙二醇中的至少一种交联剂。
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Patent Agency Ranking
公开(公告)号：US20100203101A1
公开(公告)日：2010-08-12
申请号：US12301881
申请日：2007-05-31
Applicant: Ranieri CANCEDDA , Maddalena MASTROGIACOMO , Marco SCALA
Inventor： Ranieri CANCEDDA , Maddalena MASTROGIACOMO , Marco SCALA
CPC classification number: A61L24/108 , A61L27/3834 , A61L27/3839 , A61L27/3847 , C12N5/0663 , C12N2500/42 , C12N2500/84 , C12N2501/115 , C12N2501/39 , C12N2502/115 , C12N2533/90
Abstract: A bio-membrane with angiogenic activity for implant in tissue regeneration and repair, including bone reconstruction and the repair of skin and soft tissue lesions is described, essentially constituted by a gel able to provide support and growth and/or differentiation and/or angiogenic factors for the full in vivo functionality of the cell, containing also mesenchymal stem/precursor cells, an implant device for reconstructive surgery of bone tissue, of skin and soft tissue lesions which comprises the bio-membrane, and a method for its obtainment. Use of the gel alone for tissue regeneration and of adhesive plasters that comprise it is also described.
Abstract translation: 描述了具有用于植入物在组织再生和修复中的血管生成活性的生物膜，包括骨重建和皮肤和软组织损伤的修复，其基本上由能够提供支持和生长和/或分化和/或血管生成因子的凝胶构成 用于包含间充质干/前体细胞的细胞的完整体内功能，用于骨组织重建手术，包含生物膜的皮肤和软组织损伤的植入装置及其获得方法。 还描述了单独使用凝胶用于组织再生和包含它的粘合剂膏剂。
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公开(公告)号：US20120141559A1
公开(公告)日：2012-06-07
申请号：US13370554
申请日：2012-02-10
Applicant: Ranieri CANCEDDA , Maddalena MASTROGIACOMO , Marco SCALA
Inventor： Ranieri CANCEDDA , Maddalena MASTROGIACOMO , Marco SCALA
CPC classification number: A61L24/108 , A61L27/3834 , A61L27/3839 , A61L27/3847 , C12N5/0663 , C12N2500/42 , C12N2500/84 , C12N2501/115 , C12N2501/39 , C12N2502/115 , C12N2533/90
Abstract: A bio-membrane with angiogenic activity for implant in tissue regeneration and repair, including bone reconstruction and the repair of skin and soft tissue lesions is described, essentially constituted by a gel able to provide support and growth and/or differentiation and/or angiogenic factors for the full in vivo functionality of the cell, containing also mesenchymal stem/precursor cells, an implant device for reconstructive surgery of bone tissue, of skin and soft tissue lesions which comprises the bio-membrane, and a method for its obtainment. Use of the gel alone for tissue regeneration and of adhesive plasters that comprise it is also described.
Abstract translation: 描述了具有用于植入物在组织再生和修复中的血管生成活性的生物膜，包括骨重建和皮肤和软组织损伤的修复，其基本上由能够提供支持和生长和/或分化和/或血管生成因子的凝胶构成 用于包含间充质干/前体细胞的细胞的完整体内功能，用于骨组织重建手术，包含生物膜的皮肤和软组织损伤的植入装置及其获得方法。 还描述了单独使用凝胶用于组织再生和包含它的粘合剂膏剂。
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公开(公告)号：US07109032B2
公开(公告)日：2006-09-19
申请号：US10614191
申请日：2003-07-08
Applicant: Ranieri Cancedda , Beatrice Dozin
Inventor： Ranieri Cancedda , Beatrice Dozin
CPC classification number: C12N5/0655 , A61K35/12 , C12N5/0663 , C12N2500/25 , C12N2500/36 , C12N2500/38 , C12N2500/90 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/125 , C12N2501/135 , C12N2501/235 , C12N2501/39
Abstract: Serum-free media for growth and proliferation of chondrocytes and mesenchymal stem cells in culture are provided. A serum-free medium for growth of chondrocytes includes a serum-free composition comprising FGF-2, linoleic acid, ascorbic acid, B-mercaptoethanol, transferrin and dexamethasone. Further, the composition comprises EGF, PDGFbb, insulin and albumin. A method for growing chondrocytes in a serum free medium comprising the compostion is also provided. Also provided for mesenchymal stem cell growth, is a serum-free medium which includes a composition comprising FGF-2, LIF, SCF, pantotenate, biotin and selenium and method, therefore.
Abstract translation: 提供了用于培养中软骨细胞和间充质干细胞生长和增殖的无血清培养基。 用于生长软骨细胞的无血清培养基包括含有FGF-2，亚油酸，抗坏血酸，B-巯基乙醇，转铁蛋白和地塞米松的无血清组合物。 此外，组合物包含EGF，PDGFbb，胰岛素和白蛋白。 还提供了在包含组合物的无血清培养基中培养软骨细胞的方法。 也提供用于间充质干细胞生长，是一种无血清培养基，其包括包含FGF-2，LIF，SCF，泛酸盐，生物素和硒的组合物和方法。
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公开(公告)号：US06617159B1
公开(公告)日：2003-09-09
申请号：US09831161
申请日：2001-06-11
Applicant: Ranieri Cancedda , Beatrice Dozin
Inventor： Ranieri Cancedda , Beatrice Dozin
IPC: C12N500
CPC classification number: C12N5/0655 , A61K35/12 , C12N5/0663 , C12N2500/25 , C12N2500/36 , C12N2500/38 , C12N2500/90 , C12N2501/105 , C12N2501/11 , C12N2501/115 , C12N2501/125 , C12N2501/135 , C12N2501/235 , C12N2501/39
Abstract: Serum free media for growth and proliferation of chondrocytes and mesenchymal stem cells in culture are provided. A serum free medium for growth of chondrocytes includes a serum free composition comprising FGF-2, linoleic acid, ascorbic acid, B-mercaptoethanol, transferrin and dexamethasone. Further the composition comprises EGF, PDGFbb, insulin and albumin. A method for growing chondrocytes in a serum free medium comprising the composition is also provided. Also provided for mesenchymal stem cell growth, is a serum free medium which includes a composition comprising FGF-2, LIF, SCF, pantotenate, biotin and selenium and method, therefore.
Abstract translation: 提供了培养物中软骨细胞和间充质干细胞生长和增殖的无血清培养基。 用于生长软骨细胞的无血清培养基包括含有FGF-2，亚油酸，抗坏血酸，B-巯基乙醇，转铁蛋白和地塞米松的无血清组合物。 此外，组合物包含EGF，PDGFbb，胰岛素和白蛋白。 还提供了在含有该组合物的无血清培养基中生长软骨细胞的方法。 也提供用于间充质干细胞生长，是含有FGF-2，LIF，SCF，泛酸盐，生物素和硒的组合物的无血清培养基和方法。
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公开(公告)号：US5298417A
公开(公告)日：1994-03-29
申请号：US799752
申请日：1991-11-25
Applicant: Ranieri Cancedda , Michele De Luca
Inventor： Ranieri Cancedda , Michele De Luca
IPC: C12N5/06 , A01N1/02 , A01N25/02 , A61L27/00 , A61L27/60 , C12N5/00 , C12N5/02 , A01N37/18 , A61K37/00
CPC classification number: A01N1/0284 , A01N1/02 , A01N1/0221 , A61L27/60
Abstract: A process for preserving cultured epidermal sheets by incubating the sheets at room temperature in media with a glycerol or dimethylsulfoxide cryopreservant for a predetermined period of time and then freezing the cultured sheets. The cooling gradient is characterized as having a lower initial rate of cooling followed by a higher rate of cooling.
Abstract translation: 通过在室温下将培养基中的片与甘油或二甲基亚砜冷冻保存预定的时间并然后冷冻培养的片材来保存培养的表皮片的方法。 冷却梯度的特征在于具有较低的初始冷却速率，之后是较高的冷却速率。
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