公开(公告)号：US09090887B2

公开(公告)日：2015-07-28

申请号：US12479416

申请日：2009-06-05

Applicant: William A Cuevas ,  Sang-Kyu Lee ,  Sandra W Ramer ,  Andrew Shaw ,  Amr R Toppozada ,  David E Estell ,  Louise Wallace ,  Regina Chin ,  Carol A Requadt ,  Scott D Power ,  Michael J Pepsin

Inventor： William A Cuevas ,  Sang-Kyu Lee ,  Sandra W Ramer ,  Andrew Shaw ,  Amr R Toppozada ,  David E Estell ,  Louise Wallace ,  Regina Chin ,  Carol A Requadt ,  Scott D Power ,  Michael J Pepsin

IPC: C12N9/26 ,  C12N9/28 ,  G01N11/00

CPC classification number: C12N9/2417 ,  A21D8/042 ,  C12N9/2408 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  G01N11/00 ,  Y02E50/17

Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.

Abstract translation: 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。

公开(公告)号：US06297037B1

公开(公告)日：2001-10-02

申请号：US08194664

申请日：1994-02-10

Applicant: Christopher C. Barnett ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

Inventor： Christopher C. Barnett ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

IPC: C12N928

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12N15/75 ,  C12P19/14

Abstract: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

Abstract translation: 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。

公开(公告)号：US20100003366A1

公开(公告)日：2010-01-07

申请号：US12479416

申请日：2009-06-05

Applicant: William A. Cuevas ,  Sang-Kyu Lee ,  Sandra W. Ramer ,  Andrew Shaw ,  Amr R. Toppozada ,  David E. Estell ,  Louise Wallace ,  Regina Chin ,  Carol A. Requadt ,  Scott D. Power ,  Michael J. Pepsin

Inventor： William A. Cuevas ,  Sang-Kyu Lee ,  Sandra W. Ramer ,  Andrew Shaw ,  Amr R. Toppozada ,  David E. Estell ,  Louise Wallace ,  Regina Chin ,  Carol A. Requadt ,  Scott D. Power ,  Michael J. Pepsin

IPC: A21D8/04 ,  C12P19/14 ,  C12P7/06 ,  C12P19/24

CPC classification number: C12N9/2417 ,  A21D8/042 ,  C12N9/2408 ,  C12P7/14 ,  C12P19/02 ,  C12P19/14 ,  G01N11/00 ,  Y02E50/17

Abstract: Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.

Abstract translation: 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。

公开(公告)号：US5824532A

公开(公告)日：1998-10-20

申请号：US468220

申请日：1995-06-06

Applicant: Christopher C. Barnett ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

Inventor： Christopher C. Barnett ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

IPC: C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N15/56 ,  C12N15/75 ,  C12P19/14 ,  C12N15/63

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12N15/75 ,  C12P19/14

Abstract: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

Abstract translation: 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。

公开(公告)号：US09040278B2

公开(公告)日：2015-05-26

申请号：US12478266

申请日：2009-06-04

Applicant: Luis G. Cascao-Pereira ,  Regina Chin ,  William A. Cuevas ,  David A. Estell ,  Sang-Kyu Lee ,  Michael J. Pepsin ,  Scott D. Power ,  Sandra W. Ramer ,  Carol A. Requadt ,  Andrew Shaw ,  Amr R. Toppozada ,  Louise Wallace

Inventor： Luis G. Cascao-Pereira ,  Regina Chin ,  William A. Cuevas ,  David A. Estell ,  Sang-Kyu Lee ,  Michael J. Pepsin ,  Scott D. Power ,  Sandra W. Ramer ,  Carol A. Requadt ,  Andrew Shaw ,  Amr R. Toppozada ,  Louise Wallace

IPC: C12N9/28 ,  C12P19/14 ,  C12P7/06 ,  C08B37/00 ,  C07H21/04 ,  C12P19/02 ,  C11D3/22 ,  D06L1/14 ,  C11D3/386

CPC classification number: C12P19/02 ,  C11D3/221 ,  C11D3/386 ,  C12N9/2417 ,  D06L1/14 ,  Y02E50/17

Abstract: An α-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.

Abstract translation: 来自枯草芽孢杆菌（AmyE）的α-淀粉酶从各种碳水化合物底物（包括植物淀粉，麦芽庚糖和麦芽三糖）产生显着量的葡萄糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。

公开(公告)号：US20090305935A1

公开(公告)日：2009-12-10

申请号：US12478266

申请日：2009-06-04

Applicant: Luis G. CASCAO-PEREIRA ,  Regina CHIN ,  William A. CUEVAS ,  David A. ESTELL ,  Sang-Kyu LEE ,  Michael J. PEPSIN ,  Scott D. POWER ,  Sandra W. RAMER ,  Carol A. REQUADT ,  Andrew SHAW ,  Amr R. TOPPOZADA ,  Louise WALLACE

Inventor： Luis G. CASCAO-PEREIRA ,  Regina CHIN ,  William A. CUEVAS ,  David A. ESTELL ,  Sang-Kyu LEE ,  Michael J. PEPSIN ,  Scott D. POWER ,  Sandra W. RAMER ,  Carol A. REQUADT ,  Andrew SHAW ,  Amr R. TOPPOZADA ,  Louise WALLACE

IPC: C11D3/386 ,  C12P19/14 ,  C12P7/14 ,  C12P19/24 ,  D06L1/00

CPC classification number: C12P19/02 ,  C11D3/221 ,  C11D3/386 ,  C12N9/2417 ,  D06L1/14 ,  Y02E50/17

Abstract: An α-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.

Abstract translation: 来自枯草芽孢杆菌（AmyE）的α-淀粉酶从各种碳水化合物底物（包括植物淀粉，麦芽七糖和麦芽三糖）产生显着量的葡萄糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。

公开(公告)号：US5849549A

公开(公告)日：1998-12-15

申请号：US468698

申请日：1995-06-06

Applicant: Christopher C. Barnett ,  Leif P. Solheim ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

Inventor： Christopher C. Barnett ,  Leif P. Solheim ,  Colin Mitchinson ,  Scott D. Power ,  Carol A. Requadt

IPC: C12N15/09 ,  C11D3/386 ,  C12N1/21 ,  C12N5/10 ,  C12N9/26 ,  C12N9/28 ,  C12N15/56 ,  C12N15/75 ,  C12P19/14

CPC classification number: C12N9/2417 ,  C11D3/386 ,  C12N15/75 ,  C12P19/14

Abstract: Novel alpha-amylase mutants derived from the DNA sequences of naturally occurring or recombinant alpha-amylases are disclosed. The mutant alpha-amylases, in general, are obtained by in vitro modifications of a precursor DNA sequence encoding the naturally occurring or recombinant alpha-amylase to generate the substitution (replacement) or deletion of one or more oxidizable amino acid residues in the amino acid sequence of a precursor alpha-amylase. Such mutant alpha-amylases have altered oxidative stability and/or altered pH performance profiles and/or altered thermal stability as compared to the precursor. Also disclosed are detergent and starch liquefaction compositions comprising the mutant amylases, as well as methods of using the mutant amylases.

Abstract translation: 公开了衍生自天然存在或重组α-淀粉酶的DNA序列的新型α-淀粉酶突变体。 通常，通过体外修饰编码天然存在或重组α-淀粉酶的前体DNA序列以产生氨基酸中一个或多个可氧化氨基酸残基的取代（置换）或缺失，获得突变体α-淀粉酶 前体α-淀粉酶的序列。 与前体相比，这种突变型α-淀粉酶具有改变的氧化稳定性和/或改变的pH性能特征和/或改变的热稳定性。 还公开了包含突变淀粉酶的洗涤剂和淀粉液化组合物，以及使用突变淀粉酶的方法。