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1.发明授权公开(公告)号：US09090887B2
公开(公告)日：2015-07-28
申请号：US12479416
申请日：2009-06-05
申请人： William A Cuevas , Sang-Kyu Lee , Sandra W Ramer , Andrew Shaw , Amr R Toppozada , David E Estell , Louise Wallace , Regina Chin , Carol A Requadt , Scott D Power , Michael J Pepsin
发明人： William A Cuevas , Sang-Kyu Lee , Sandra W Ramer , Andrew Shaw , Amr R Toppozada , David E Estell , Louise Wallace , Regina Chin , Carol A Requadt , Scott D Power , Michael J Pepsin
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
摘要： Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
摘要翻译： 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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2.发明申请公开(公告)号：US20100003366A1
公开(公告)日：2010-01-07
申请号：US12479416
申请日：2009-06-05
申请人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Louise Wallace , Regina Chin , Carol A. Requadt , Scott D. Power , Michael J. Pepsin
发明人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Louise Wallace , Regina Chin , Carol A. Requadt , Scott D. Power , Michael J. Pepsin
CPC分类号： C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
摘要： Alpha-amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
摘要翻译： 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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公开(公告)号：US20100105598A1
公开(公告)日：2010-04-29
申请号：US12445313
申请日：2007-10-12
CPC分类号： C11D3/38609 , C11D3/38618
摘要： The present invention provides non-phosphate containing dishwashing detergent compositions. The present invention also provides methods for the production of and use of such detergents.
摘要翻译： 本发明提供了含磷酸盐的餐具洗涤剂组合物。 本发明还提供了生产和使用这种洗涤剂的方法。
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4.发明申请Geobacillus Stearothermophilus Alpha-Amylase (AmyS) Variants with Improved Properties 有权具有改善性质的嗜热脂肪芽孢杆菌α-淀粉酶（AmyS）变体公开(公告)号：US20090314286A1
公开(公告)日：2009-12-24
申请号：US12477028
申请日：2009-06-02
申请人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Sura H. Hadi , Walter Weyler
发明人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David E. Estell , Sura H. Hadi , Walter Weyler
CPC分类号： D06M16/003 , C11D3/38609 , C11D3/38618 , C11D3/3869 , C12N9/2417 , C12Y302/01001 , D06L4/40
摘要： Described are variants of a parent α-amylase that exhibits an alteration in at least one of the following properties relative to said parent α-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.
摘要翻译： 描述了相对于所述亲本α-淀粉酶在下列性质中的至少一个表现出改变的母体α-淀粉酶的变体：比活性，底物特异性，底物结合，底物切割，热稳定性，pH依赖性活性，pH 依赖稳定性，氧化稳定性，Ca2 +依赖性，pI和洗涤性能。 这些变体适用于淀粉转化，乙醇生产，洗衣，洗碗，硬表面清洗，纺织品退浆和/或甜味剂生产。
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5.发明申请GEOBACILLUS STEAROTHERMOPHILUS ALPHA AMYLASE (AMYS) VARIANTS WITH IMPROVED PROPERTIES 审中-公开具有改进性质的GEOBACILLUS STEAROTHERMOPHILUS ALPHA AMYLASE（AMYS）变种公开(公告)号：US20120156733A1
公开(公告)日：2012-06-21
申请号：US13291938
申请日：2011-11-08
申请人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David A. Estell , Sura Hadi
发明人： William A. Cuevas , Sang-Kyu Lee , Sandra W. Ramer , Andrew Shaw , Amr R. Toppozada , David A. Estell , Sura Hadi
CPC分类号： D06M16/003 , C11D3/38609 , C11D3/38618 , C11D3/3869 , C12N9/2417 , C12Y302/01001 , D06L4/40
摘要： Described are variants of a parent α-amylase that exhibits an alteration in at least one of the following properties relative to said parent α-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.
摘要翻译： 描述了亲本α-淀粉酶的变体，其相对于所述亲本α-淀粉酶在至少一个以下性质中具有改变：比活性，底物特异性，底物结合，底物切割，热稳定性，pH依赖性活性，pH 依赖稳定性，氧化稳定性，Ca2 +依赖性，pI和洗涤性能。 这些变体适用于淀粉转化，乙醇生产，洗衣，洗碗，硬表面清洗，纺织品退浆和/或甜味剂生产。
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6.发明授权Production of glucose from starch using alpha-amylases from Bacillus subtilis 有权使用来自枯草芽孢杆菌的α-淀粉酶从淀粉生产葡萄糖公开(公告)号：US09040278B2
公开(公告)日：2015-05-26
申请号：US12478266
申请日：2009-06-04
申请人： Luis G. Cascao-Pereira , Regina Chin , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Michael J. Pepsin , Scott D. Power , Sandra W. Ramer , Carol A. Requadt , Andrew Shaw , Amr R. Toppozada , Louise Wallace
发明人： Luis G. Cascao-Pereira , Regina Chin , William A. Cuevas , David A. Estell , Sang-Kyu Lee , Michael J. Pepsin , Scott D. Power , Sandra W. Ramer , Carol A. Requadt , Andrew Shaw , Amr R. Toppozada , Louise Wallace
IPC分类号： C12N9/28 , C12P19/14 , C12P7/06 , C08B37/00 , C07H21/04 , C12P19/02 , C11D3/22 , D06L1/14 , C11D3/386
摘要： An α-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
摘要翻译： 来自枯草芽孢杆菌（AmyE）的α-淀粉酶从各种碳水化合物底物（包括植物淀粉，麦芽庚糖和麦芽三糖）产生显着量的葡萄糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。
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公开(公告)号：US20120094867A1
公开(公告)日：2012-04-19
申请号：US13320879
申请日：2010-05-25
申请人： Sang-Kyu Lee , Sandra W. Ramer , Amr R. Toppozada
发明人： Sang-Kyu Lee , Sandra W. Ramer , Amr R. Toppozada
IPC分类号： C40B30/10
CPC分类号： G01N11/14 , G01N2011/008
摘要： Described are compositions and methods related to determining the rate of viscosity change in a suspension in real time. The compositions and methods have a broad range of applications, including the measurement of amylase-mediated liquefaction of a starch suspension.
摘要翻译： 描述了与实时确定悬浮液中粘度变化速率有关的组合物和方法。 组合物和方法具有广泛的应用范围，包括淀粉酶介导的淀粉悬浮液液化的测量。
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8.发明授权Geobacillus stearothermophilus α-amylase (AmyS) variants with improved properties 有权具有改善性质的嗜热脂肪芽孢杆菌α-淀粉酶（AmyS）变体公开(公告)号：US08084240B2
公开(公告)日：2011-12-27
申请号：US12477028
申请日：2009-06-02
申请人： William A Cuevas , Sang-Kyu Lee , Sandra W Ramer , Andrew Shaw , Amr R Toppozada , David E Estell , Sura H Hadi
发明人： William A Cuevas , Sang-Kyu Lee , Sandra W Ramer , Andrew Shaw , Amr R Toppozada , David E Estell , Sura H Hadi
CPC分类号： D06M16/003 , C11D3/38609 , C11D3/38618 , C11D3/3869 , C12N9/2417 , C12Y302/01001 , D06L4/40
摘要： Described are variants of a parent α-amylase that exhibits an alteration in at least one of the following properties relative to said parent α-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, Ca2+ dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.
摘要翻译： 描述了亲本α-淀粉酶的变体，其相对于所述亲本α-淀粉酶在至少一个以下性质中具有改变：比活性，底物特异性，底物结合，底物切割，热稳定性，pH依赖性活性，pH 依赖稳定性，氧化稳定性，Ca2 +依赖性，pI和洗涤性能。 这些变体适用于淀粉转化，乙醇生产，洗衣，洗碗，硬表面清洗，纺织品退浆和/或甜味剂生产。
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9.发明申请PRODUCTION OF GLUCOSE FROM STARCH USING ALPHA-AMYLASES FROM BACILLUS SUBTILIS 有权使用来自BACILLUS SUBTILIS的ALPHA-AMYLASES从淀粉生产葡萄糖公开(公告)号：US20090305935A1
公开(公告)日：2009-12-10
申请号：US12478266
申请日：2009-06-04
申请人： Luis G. CASCAO-PEREIRA , Regina CHIN , William A. CUEVAS , David A. ESTELL , Sang-Kyu LEE , Michael J. PEPSIN , Scott D. POWER , Sandra W. RAMER , Carol A. REQUADT , Andrew SHAW , Amr R. TOPPOZADA , Louise WALLACE
发明人： Luis G. CASCAO-PEREIRA , Regina CHIN , William A. CUEVAS , David A. ESTELL , Sang-Kyu LEE , Michael J. PEPSIN , Scott D. POWER , Sandra W. RAMER , Carol A. REQUADT , Andrew SHAW , Amr R. TOPPOZADA , Louise WALLACE
摘要： An α-amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
摘要翻译： 来自枯草芽孢杆菌（AmyE）的α-淀粉酶从各种碳水化合物底物（包括植物淀粉，麦芽七糖和麦芽三糖）产生显着量的葡萄糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。
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