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    HYBRID ALPHA-AMYLASES
    4.
    发明申请
    HYBRID ALPHA-AMYLASES 有权
    混合阿尔法胺

    公开(公告)号：US20120021485A1

    公开(公告)日：2012-01-26

    申请号：US13132881

    申请日：2009-12-11

    Applicant: Scott D. Power ,  Andrew Shaw

    Inventor： Scott D. Power ,  Andrew Shaw

    IPC: C12N9/96 ,  G06G7/60 ,  C12N1/21 ,  C12N1/15 ,  C12N15/62 ,  C12N15/63

    CPC classification number: C12N9/2417 ,  C07K2319/00 ,  G06F19/12 ,  G06F19/16

    Abstract: Hybrid alpha-amylases are provided that share a conserved 3D structure in whole or in part with a wild-type Termamyl-like ?-amylase, e.g., a Bacillus amylase. In the hybrid, an N terminal portion of a Termamyl-like ?-amylase is replaced with sequences from an archae ? amylase. The sequence similarity between the two amylase sequences may be less than 60%. Conserving the wild-type 3D structure in the hybrid facilitates obtaining enzymatically active amylases. In one embodiment, one or both amylase sequences contribute residues to the B domain, resulting in particularly advantageous properties. For instance, replacement of the Ca2+ binding site in the B domain of the Termamyl-like ?-amylase with a B domain sequence of an archae ? amylase that does not bind Ca2+ can produce a hybrid that is fully active in the absence of Ca2+.

    Abstract translation: 提供杂交α-淀粉酶，其与野生型类戊酰样α-淀粉酶（例如芽孢杆菌淀粉酶）全部或部分共享保守的3D结构。 在杂交体中，类戊酰样α-淀粉酶的N末端部分被来自古细菌的序列替代 淀粉酶。 两个淀粉酶序列之间的序列相似性可以小于60％。 在杂交中保护野生型3D结构有助于获得酶活性淀粉酶。 在一个实施方案中，一个或两个淀粉酶序列向B结构域贡献残基，导致特别有利的性质。 例如，用具有古细菌的B结构域序列的Termamyl样α-淀粉酶的B结构域中的Ca 2+结合位点的替换 不结合Ca2 +的淀粉酶可以产生在不存在Ca 2+的情况下完全活性的杂交体。

    Production of functional antibodies in filamentous fungi
    5.
    发明授权
    Production of functional antibodies in filamentous fungi 有权
    生产丝状真菌中的功能性抗体

    公开(公告)号：US07977067B2

    公开(公告)日：2011-07-12

    申请号：US11007886

    申请日：2004-12-09

    Applicant: Scott D. Power ,  Huaming Wang ,  Michael Ward

    Inventor： Scott D. Power ,  Huaming Wang ,  Michael Ward

    IPC: C07K16/46

    CPC classification number: C07K16/2818 ,  C07K16/00 ,  C07K16/32 ,  C07K2317/10 ,  C07K2317/41 ,  C07K2317/55 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2319/00

    Abstract: Described herein are methods for the production of monoclonal antibodies in filamentous fungi host cells. The monoclonal antibodies are expressed as full-length fusion proteins that retain functional antigen binding and antibody-dependent cellular cytotoxicity capabilities. Improvements in the cleavage of the glucoamylase-light chain fusion protein to yield a mature antibody are also provided. The antibodies produced in filamentous fungi show equivalent pharmacokinetic disposition to antibodies produced in mammalian cells.

    Abstract translation: 本文描述了在丝状真菌宿主细胞中生产单克隆抗体的方法。 单克隆抗体表达为全长融合蛋白，其保留功能性抗原结合和抗体依赖性细胞毒性能力。 还提供了葡糖淀粉酶 - 轻链融合蛋白切割产生成熟抗体的改进。 在丝状真菌中产生的抗体对哺乳动物细胞中产生的抗体显示出等效的药代动力学位置。

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