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    METHOD FOR ESTIMATING A MELTING TEMPERATURE OF A NUCLEIC ACID IN BUFFERS CONTAINING MAGNESIUM IONS
    5.
    发明申请
    METHOD FOR ESTIMATING A MELTING TEMPERATURE OF A NUCLEIC ACID IN BUFFERS CONTAINING MAGNESIUM IONS 有权
    用于估计含有镁离子的缓冲液中核酸的熔融温度的方法

    公开(公告)号：US20090198453A1

    公开(公告)日：2009-08-06

    申请号：US11970509

    申请日：2008-01-07

    申请人： Richard Owczarzy ,  Bernardo Moreira ,  Yong You ,  Mark Aaron Behlke ,  Joseph Alan Walder

    发明人： Richard Owczarzy ,  Bernardo Moreira ,  Yong You ,  Mark Aaron Behlke ,  Joseph Alan Walder

    IPC分类号： G06F19/00

    CPC分类号： G06F19/20 ,  G06F19/18

    摘要： The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, in the presence of divalent cations, particularly duplexes of oligonucleotides which may be used as, for example, but not limited to primers or probes in PCR and/or hybridization assays. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of divalent cation salt conditions on the melting temperature.

    摘要翻译： 本发明涉及用于预测或估计双链核酸的解链温度的方法和系统，所述方法和系统在二价阳离子，特别是寡核苷酸的双链体存在下可用作例如但不限于PCR和/ 或杂交测定。 方法和算法使用具有作为特定核苷酸序列的功能的术语和系数的新型公式来估计二价阳离子盐条件对熔融温度的影响。

    Methods and systems for estimating the melting temperature (TM) for polynucleotide molecules
    6.
    发明授权
    Methods and systems for estimating the melting temperature (TM) for polynucleotide molecules 有权
    用于估计多核苷酸分子的熔解温度（TM）的方法和系统

    公开(公告)号：US06889143B2

    公开(公告)日：2005-05-03

    申请号：US10660253

    申请日：2003-09-11

    申请人： Mark Aaron Behlke ,  Lingyan Huang ,  Richard Owczarzy ,  Joseph Alan Walder

    发明人： Mark Aaron Behlke ,  Lingyan Huang ,  Richard Owczarzy ,  Joseph Alan Walder

    IPC分类号： C12Q1/68 ,  G01N20060101 ,  G01N33/48 ,  G01N33/50 ,  G06F17/00 ,  G06F17/10 ,  G06F17/11 ,  G06F19/20 ,  G06F19/22

    CPC分类号： G06F19/20 ,  C12Q1/6813 ,  G06F19/22

    摘要： The invention relates to methods and systems for predicting or estimating the melting temperature of duplex nucleic acids, particularly duplexes of oligonucleotides which may be used, for example, as primers or probes in PCR and/or hybridization assays. The invention also relates to methods and systems for designing and selecting oligonucleotide probes and primers having a predicted melting temperature which is optimized for such assays. To this end, algorithms and methods are provided for predicting the melting temperature of a nucleic acid having a predetermined sequence. These methods and algorithms estimate the melting temperature of a nucleic acid duplex under particular salt conditions. The methods and algorithms use novel formulas, having terms and coefficients that are functions of the particular nucleotide sequence, to estimate the effect of particular salt conditions on the melting temperature. As such, the methods and systems of the invention provide superior result compared to existing methods, which do not consider sequence dependent effects of changing salt conditions.

    摘要翻译： 本发明涉及用于预测或估计双链体核酸，特别是寡核苷酸双链体的解链温度的方法和系统，其可用于例如PCR和/或杂交测定中的引物或探针。 本发明还涉及用于设计和选择寡核苷酸探针和引物的方法和系统，所述寡核苷酸探针和引物具有针对这种测定法优化的预测的熔融温度。 为此，提供了用于预测具有预定序列的核酸的解链温度的算法和方法。 这些方法和算法估计在特定盐条件下核酸双链体的解链温度。 方法和算法使用具有作为特定核苷酸序列的功能的术语和系数的新型公式来估计特定盐条件对熔融温度的影响。 因此，与现有方法相比，本发明的方法和系统提供了优异的结果，其不考虑改变盐条件的序列依赖性影响。