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1.发明授权Process for the incorporation of foreign DNA into the genome of dicotyledonous plants using stable cointegrate plasmids 失效使用稳定的协整质粒将外来DNA掺入双子叶植物基因组的方法
公开(公告)号：US4693976A
公开(公告)日：1987-09-15
申请号：US583024
申请日：1984-02-23
IPC分类号： C12N15/09 , A01H1/00 , A01H5/00 , C12N1/21 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/82 , C12R1/01 , C12R1/19 , C12P21/00 , C12N1/00 , C12N1/22 , C12P21/02
CPC分类号： C12N15/8205
摘要： The invention relates to a process for the incorporation of foreign DNA into the genome of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium tumefaciens bacteria, which contain one or more Ti (tumour inducing) plasmids, wherein as Ti plasmid a stable cointegrate plasmid composed of the plasmid R772 and the plasmid pTiB6 with foreign DNA incorporated in the T-region of the Ti component of the cointegrate plasmid is applied as well as to a cointegrate plasmid pAL969 and cointegrate plasmids derived from the cointegrate plasmid by the incorporation of foreign DNA into the T-region of the Ti component.
摘要翻译： 本发明涉及通过用含有一种或多种Ti（诱导肿瘤）质粒的根癌土壤杆菌细菌感染植物或培养植物原生质体将外来DNA并入双子叶植物的基因组的方法，其中作为Ti质粒是稳定的协整 将由质粒R772组成的质粒和掺入到协整质粒的Ti成分的T区中的外源DNA的质粒pTiB6应用于协整质粒pAL969，并通过掺入外源并入源自协整质粒的质粒 DNA进入Ti组分的T区。
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2.发明授权Process for the incorporation of foreign DNA into the genome of dicotyledonous plants 失效将外来DNA掺入双子叶植物基因组的方法
公开(公告)号：US5464763A
公开(公告)日：1995-11-07
申请号：US173271
申请日：1993-12-23
IPC分类号： A01H1/00 , A01H5/00 , C12N1/20 , C12N1/21 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/82 , C12R1/01 , C12N15/84 , C12N15/74
CPC分类号： C12N15/8205
摘要： The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of Ti (tumor inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria, suitable for use in the process according to claim 1 wherein at least one plasmid which has the vir-region of a Ti (tumor inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.
摘要翻译： 本发明涉及通过用含有一种或多种质粒的农杆菌细菌感染植物或培养植物原生质体将外来DNA掺入双子叶植物的染色体的方法，其中使用含有至少一种具有vir- Ti（肿瘤诱导）质粒的区域，但没有T区，以及至少一种其他具有T区的外源DNA但不含有vir区的质粒，以及适用于该方法的农杆菌 根据权利要求1的方法，其中至少一种具有Ti（诱导肿瘤）质粒但不具有T区的vir区的至少一种质粒和至少一种具有野生型T区的其它质粒，其中掺入外源DNA但不含 vir区域。
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公开(公告)号：US5019501A
公开(公告)日：1991-05-28
申请号：US317062
申请日：1989-02-28
CPC分类号： C12N15/64
摘要： A process for the activation of genes in micro-organisms, in which genes not occurring as such in nature are activated by contact with plants or with the substance or substances causing the activation and present or not in plant fragments or plant exudates, the genes used consisting of one or more proteins encoding fragments of one or more natural genes and/or one or more synthetically produced DNA-sequences, which encode for one or more proteins and a promotor region originating from one of the genes naturally present in micro-organisms and being of such a nature that respectively the effecting or omitting of the above-mentioned contact makes it possible to activate the artificially constructed gene or genes or to stop the activity of the gene or genes, respectively.
摘要翻译： 用于激活微生物中的基因的方法，其中本质上不发生的基因通过与植物接触或引起活化的物质或物质而活化，并且存在或不存在于植物碎片或植物渗出物中，所用基因 由编码一种或多种蛋白质的一种或多种天然基因片段和/或一种或多种合成产生的DNA序列的一种或多种蛋白质组成，所述DNA序列编码一种或多种蛋白质，以及源自天然存在于微生物中的一种基因的启动子区域，以及 具有这样的性质，分别实现或省略上述接触使得能够激活人造构建的基因或基因或分别停止基因或基因的活性。
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4.发明授权Process for the incorporation of foreign dna into the genome of dicotyledonous plants 失效将外来dna掺入双子叶植物基因组的方法
公开(公告)号：US4940838A
公开(公告)日：1990-07-10
申请号：US583022
申请日：1984-02-23
IPC分类号： A01H1/00 , A01H5/00 , C12N1/20 , C12N1/21 , C12N5/00 , C12N5/10 , C12N15/00 , C12N15/09 , C12N15/82 , C12R1/01
CPC分类号： C12N15/8205
摘要： The invention relates to a process for the incorporation of foreign DNA into chromosomes of dicotyledonous plants by infecting the plants or incubating plant protoplasts with Agrobacterium bacteria, which contain one or more plasmids, wherein bacteria are used which contain at least one plasmid having the vir-region of a Ti (tumour inducing) plasmid but no T-region, and at least one other plasmid having a T-region with incorporated therein foreign DNA but no vir-region, as well as to a Agrobacterium bacteria wherein at least one plasmid which has the vir-region of a Ti (tumour inducing) plasmid but no T-region and at least one other plasmid which has a wild type T-region with incorporated in it foreign DNA but no vir-region.
摘要翻译： 本发明涉及通过用含有一种或多种质粒的农杆菌细菌感染植物或培养植物原生质体将外来DNA掺入双子叶植物的染色体的方法，其中使用含有至少一种具有vir- Ti（肿瘤诱导）质粒但不含T区的区域，以及至少一种具有T区的外源DNA但不含有vir区的其他质粒，以及至少一种质粒， 具有Ti（肿瘤诱导）质粒的vir区域，但没有T区和至少一个具有野生型T区的其他质粒，其中掺入外源DNA但没有vir区。
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5.发明授权Process for the in-vitro transformation of plant protoplasts with plasmid DNA 失效用质粒DNA体外转化植物原生质体的方法
公开(公告)号：US4684611A
公开(公告)日：1987-08-04
申请号：US760145
申请日：1985-07-29
IPC分类号： C12P13/04 , A01H1/00 , A01H5/00 , C12N5/00 , C12N5/10 , C12N9/00 , C12N15/00 , C12N15/09 , C12N15/63 , C12N15/82 , C12P5/00 , C12P21/00 , C12P33/00 , C12R1/91
CPC分类号： C12N15/63 , C12N15/8206
摘要： Genetic properties of higher plants are transformed by incubating protoplasts of the plants together with plasmid DNA. The incubation is effected in the presence of polyethylene glycol and calcium ions. Subsequently--during after-incubation--the calcium ion concentration in the incubation medium is gradually increased and at the same time the polyethylene glycol concentration is decreased. The resulting aggregates are separated, grown further separately, and examined for modification of their genetic properties.
摘要翻译： 通过将植物的原生质体与质粒DNA一起孵育来转化高等植物的遗传性质。 在聚乙二醇和钙离子存在下孵育。 随后 - 在温育期间，培养基中的钙离子浓度逐渐增加，同时聚乙二醇浓度降低。 将所得聚集体分离，分开生长，并检查其遗传性质的变化。
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