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公开(公告)号：US20230368873A1
公开(公告)日：2023-11-16
申请号：US18044963
申请日：2021-06-28
摘要： An event chain reaction system (128) is disclosed. The event chain reaction system (128) comprises: —at least one communication interface (156) configured for receiving at least one event stream (160), wherein the event stream (160) comprises at least one sequence of ordered events generated by at least one analytical system (112), wherein each event comprises information about a change in a state of the analytical system (112) and/or any of loaded resources; —at least one chain reaction component (158) comprising at least one chain matching element (162), wherein the chain matching element (162) is configured for recognizing at least one chain on the event stream (160), wherein the chain comprises a set of ordered events to be searched, wherein a first event of the chain defines a start event (166), wherein the chain matching element (162) is configured for identifying the start event (166) in the event stream (160) and, upon identifying the start event (166), the chain matching element (162) is configured for successively determining whether the other events of the chain match to one of the events of the event stream (160), wherein, in case all events of the chain are matched to events of the event stream (160), the chain matching element (162) is configured for triggering at least one reaction, wherein the reaction comprises generating information that a chain was matched and/or issuing a command to at least one component of the analytical system (112), wherein, in case one of the events of the chain is not matched, the chain matching element (162) is configured for resetting to its initial state and waiting for the start event (166). Further, a system (110) for monitoring and/or controlling, a computer implemented method and a computer program for determining at least one feature of at least one component of an analytical system (112), a computer implemented method and a computer program for monitoring and/or controlling at least one feature of at least one component of an analytical system (112) are disclosed.
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公开(公告)号：US09512494B2
公开(公告)日：2016-12-06
申请号：US14055698
申请日：2013-10-16
发明人： Frank Bergmann , Dorothea Sizmann , Heike Zitzer
CPC分类号： C12Q1/707 , C07H21/04 , C12Q1/6816 , C12Q2537/143
摘要： The present invention relates to a method for amplifying and detecting a target nucleic acid of HCV in a sample, wherein an amplification of the nucleic acids in said sample is carried out. This amplification involves a polymerase, primers for generating an amplicon and at least two detectable probes specific for different sequence portions of said amplicon. Detection of the obtained amplicon is brought about by detecting hybridization of the probes mentioned above to said different sequence portions of the amplicon.The invention further provides reaction mixtures and kits for amplifying and detecting a target nucleic acid of HCV involving the use of at least two detectable probes specific for different sequence portions of an amplicon.
摘要翻译： 本发明还提供了用于扩增和检测HCV的靶核酸的反应混合物和试剂盒，其涉及使用对扩增子的不同序列部分特异的至少两种可检测探针。
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公开(公告)号：US20160348158A1
公开(公告)日：2016-12-01
申请号：US14724592
申请日：2015-05-28
发明人： Stephen Gordon Will
CPC分类号： C12Q1/6827 , C07H21/00 , C12Q2525/161 , C12Q2525/186 , C12Q2525/197 , C12Q2565/1015
摘要： The invention provides, inter alia, novel probes, methods, reaction mixtures, and kits for detecting the presence or absence of a target nucleic acid sequence.
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4.发明授权公开(公告)号：US09447476B2
公开(公告)日：2016-09-20
申请号：US14055090
申请日：2013-10-16
发明人： Reiner Babiel , Frank Bergmann , Dorothea Sizmann
CPC分类号： C12Q1/707 , C12Q1/6844 , C12Q1/6848 , C12Q1/6853 , C12Q1/686 , C12Q2525/161 , C12Q2525/173 , C12Q2531/113
摘要： The present invention is directed to improved methods for amplifying and detecting a nucleic acid target that may be present in a biological sample comprising a primer pair for generating an amplicon, wherein at least one primer is modified at the 5′ terminus with a polyN sequence being non-complementary to the target sequence, and a detectable probe specific for said amplicon or a DNA binding dye. The formation of high molecular weight products during amplification is prevented or partly or completely suppressed. The invention further provides reaction mixtures and kits comprising said primers for preventing or suppressing the formation of high molecular weight products during amplification and detection of the nucleic acid target.
摘要翻译： 本发明涉及用于扩增和检测可能存在于包含用于产生扩增子的引物对的生物样品中的核酸靶的改进方法，其中至少一个引物在5'末端被修饰，其中polyN序列是 与靶序列不互补，以及对所述扩增子或DNA结合染料具有特异性的可检测探针。 在扩增期间形成高分子量产物被预防或部分或完全抑制。 本发明还提供了反应混合物和试剂盒，其包含用于在核酸靶扩增和检测期间预防或抑制高分子量产物形成的所述引物。
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5.发明授权公开(公告)号：US08868393B2
公开(公告)日：2014-10-21
申请号：US13889262
申请日：2013-05-07
发明人： Wei-Min Liu
摘要： Methods for generating a normalized expression signal for microarray data based on a theoretical distribution at the unit level to produce a normalized expression signal for the single microarray that is independent of other microarrays. The method typically includes receiving microarray data representing a plurality of probe pairs for a single microarray, determining, for each probe pair, differences between intensities of perfect match (PM) probes and intensities of mismatched (MM) probes, determining a difference signal, D, based on the determined differences, and scaling the difference signal, D, to produce an expression signal, DS. The method also typically includes normalizing the expression signal based on a theoretical distribution at the unit level to produce a normalized expression signal for the single microarray that is independent of other microarrays.
摘要翻译： 基于单位级别的理论分布产生用于微阵列数据的归一化表达式信号以产生独立于其他微阵列的单个微阵列的归一化表达式信号的方法。 该方法通常包括接收表示用于单个微阵列的多个探针对的微阵列数据，为每个探针对确定完美匹配（PM）探针的强度和不匹配（MM）探针的强度之间的差异，确定差异信号D 基于所确定的差异，并且缩放差分信号D以产生表达信号DS。 该方法还通常包括基于在单位水平的理论分布来归一化表达信号，以产生独立于其他微阵列的单个微阵列的归一化表达信号。
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6.发明申请METHOD OF DETECTING A PRESENCE AND/OR MEASURING A QUANTITY OF AN ANALYTE IN A SAMPLE BY A NUCLEIC ACID AMPLIFICATION REACTION 审中-公开通过核酸放大反应检测样品中存在和/或测定分析物的数量的方法公开(公告)号：US20140106358A1
公开(公告)日：2014-04-17
申请号：US14051880
申请日：2013-10-11
发明人： Rolf Knobel
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2537/165
摘要： A method of detecting a presence and/or measuring a quantity of an analyte in a sample by a nucleic acid amplification reaction is disclosed, the method including acquiring a fluorescence intensity of the analyte for each amplification reaction cycle of the nucleic acid amplification reaction (810) and creating from the fluorescence intensity a growth signal being indicative of a fluorescence intensity over the cycles, determining an intercept value of the signal (820), determining a baseline of the signal (830), determining a maximum growth value of the signal (840), normalizing the signal by modifying the signal (850), wherein the modification includes calculating a difference of the signal and the baseline and dividing the difference by a divisor, wherein the divisor is proportional to a combination of the intercept and the maximum growth value, wherein the normalized signal is dimensionless, and processing (860) the normalized signal in order to determine a cycle number at which the normalized signal exceeds the baseline (870) for detecting the presence and/or measuring the quantity of the analyte (880).
摘要翻译： 公开了通过核酸扩增反应检测样品中存在和/或测量样品中的分析物的量的方法，所述方法包括获取核酸扩增反应（810）的每个扩增反应循环的分析物的荧光强度 ），并且从所述荧光强度产生生长信号，其指示所述周期上的荧光强度，确定所述信号的截距值（820），确定所述信号的基线（830），确定所述信号的最大生长值（ 840），通过修改信号（850）来对信号进行标准化，其中修改包括计算信号和基线的差异，并将差除以除数，其中除数与截距和最大增长的组合成正比 值，其中所述归一化信号是无量纲的，并且处理（860）所述归一化信号，以便确定所述归一化信号的周期数 e标准化信号超过用于检测分析物（880）的存在和/或测量量的基线（870）。
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公开(公告)号：US08759063B2
公开(公告)日：2014-06-24
申请号：US13706107
申请日：2012-12-05
发明人： Keith Bauer , Thomas W. Myers , Shawn Suko
IPC分类号： A61K38/00 , G01N33/535 , C12N15/10
CPC分类号： C12N9/1252 , C12N9/1276 , C12P19/34 , C12Q1/686 , C12Y207/07007 , C12Y207/07049
摘要： Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.
摘要翻译： 公开了相对于相应的未修饰聚合酶具有增加的逆转录酶效率的DNA聚合酶。 聚合酶可用于各种公开的引物延伸方法。 还公开了相关组合物，包括重组核酸，载体和宿主细胞，其可用于例如生产DNA聚合酶。
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公开(公告)号：US20140134611A1
公开(公告)日：2014-05-15
申请号：US14055469
申请日：2013-10-16
发明人： Frank Bergmann , Dorothea Sizmann , Heike Zitzer
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6813 , C12Q1/6816 , C12Q1/707 , C12Q2537/143
摘要： The present invention relates to a method for amplifying and detecting a target nucleic acid in a sample, said target nucleic comprising subgroups with sequence variations and/or individual mutations, wherein an amplification of the nucleic acids in said sample is carried out. This amplification involves a polymerase, primers for generating an amplicon and at least two detectable probes specific for different sequence portions of said amplicon. Detection of the obtained amplicon is brought about by detecting hybridization of the probes mentioned above to said different sequence portions of the amplicon. The invention further provides reaction mixtures and kits for amplifying and detecting a target nucleic acid comprising subgroups with sequence variations and/or individual mutations involving the use of at least two detectable probes specific for different sequence portions of an amplicon.
摘要翻译： 本发明涉及用于扩增和检测样品中靶核酸的方法，所述靶核酸包含具有序列变异和/或单个突变的亚组，其中进行所述样品中核酸的扩增。 该扩增包括聚合酶，用于产生扩增子的引物和对所述扩增子的不同序列部分特异的至少两个可检测探针。 通过检测上述探针与扩增子的不同序列部分的杂交来获得所得扩增子的检测。 本发明还提供了用于扩增和检测靶核酸的反应混合物和试剂盒，所述靶核酸包含具有序列变异和/或涉及使用对扩增子的不同序列部分特异的至少两种可检测探针的单个突变的亚组。
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公开(公告)号：US20140127673A1
公开(公告)日：2014-05-08
申请号：US14055698
申请日：2013-10-16
发明人： Frank Bergmann , Dorothea Sizmann , Heike Zitzer
IPC分类号： C12Q1/70
CPC分类号： C12Q1/707 , C07H21/04 , C12Q1/6816 , C12Q2537/143
摘要： The present invention relates to a method for amplifying and detecting a target nucleic acid of HCV in a sample, wherein an amplification of the nucleic acids in said sample is carried out. This amplification involves a polymerase, primers for generating an amplicon and at least two detectable probes specific for different sequence portions of said amplicon. Detection of the obtained amplicon is brought about by detecting hybridization of the probes mentioned above to said different sequence portions of the amplicon.The invention further provides reaction mixtures and kits for amplifying and detecting a target nucleic acid of HCV involving the use of at least two detectable probes specific for different sequence portions of an amplicon.
摘要翻译： 本发明涉及用于扩增和检测样品中HCV的靶核酸的方法，其中进行所述样品中核酸的扩增。 该扩增包括聚合酶，用于产生扩增子的引物和对所述扩增子的不同序列部分特异的至少两个可检测探针。 通过检测上述探针与扩增子的不同序列部分的杂交来获得所得扩增子的检测。 本发明还提供了用于扩增和检测HCV的靶核酸的反应混合物和试剂盒，其涉及使用对扩增子的不同序列部分特异的至少两种可检测探针。
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公开(公告)号：US20140087371A1
公开(公告)日：2014-03-27
申请号：US13624032
申请日：2012-09-21
发明人： Shi-Da Y. Lu
CPC分类号： C12Q1/689
摘要： Methods for the rapid detection of the presence or absence of Clostridium difficile in a biological or nonbiological sample are described. The methods can include performing an amplifying step, a hybridizing step, and a detecting step. Furthermore, primers, probes, and kits are provided that are designed for the detection of Clostridium difficile.
摘要翻译： 描述了在生物或非生物样品中快速检测难辨梭状芽孢杆菌存在或不存在的方法。 该方法可以包括执行放大步骤，杂交步骤和检测步骤。 此外，提供了用于检测艰难梭菌的引物，探针和试剂盒。
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