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    Classification of Organisms Based on Genome Representing Arrays
    5.
    发明申请
    Classification of Organisms Based on Genome Representing Arrays 审中-公开
    基于表达阵列基因组的生物分类

    公开(公告)号：US20080113872A1

    公开(公告)日：2008-05-15

    申请号：US11579025

    申请日：2005-04-29

    申请人： Frank Henri Johan Schuren ,  Martinus Petrus Maria Caspers ,  Bianca Johanna Catherina Van der Werff-van der Vat ,  Roy Christiaan Montijn

    发明人： Frank Henri Johan Schuren ,  Martinus Petrus Maria Caspers ,  Bianca Johanna Catherina Van der Werff-van der Vat ,  Roy Christiaan Montijn

    IPC分类号： C40B30/04 ,  C40B50/06

    CPC分类号： C12Q1/689 ,  C12Q1/6837 ,  C12Q1/6888 ,  G16B25/00 ,  C12Q2565/513

    摘要： The invention relates to a method of preparing clusters of reference hybridization patterns for a sample nucleic acid, comprising providing an array comprising a plurality of nucleic acid molecules, wherein said plurality of nucleic acid molecules is derived from at least two different sources, providing at least two different reference hybridization patterns by hybridizing said array with at least two different reference nucleic acids, wherein the sources of said at least two different reference nucleic acids are separable into at least two groups on the basis of a value for at least one phenotypic parameter, and clustering the reference hybridization patterns by unsupervised multivariate analysis. The method further provides a method for typing sample nucleic acid, comprising providing at least two different clusters of reference hybridization patterns for a sample nucleic acid by using a method according to the invention, hybridizing the same array as used for preparing the reference hybridization patterns with sample nucleic acid to obtain a sample hybridization pattern, and assigning the sample hybridization pattern to one of said at least two different clusters of reference hybridization patterns.

    摘要翻译： 本发明涉及制备用于样品核酸的参考杂交模式簇的方法，其包括提供包含多个核酸分子的阵列，其中所述多个核酸分子衍生自至少两个不同的来源，至少提供 通过将所述阵列与至少两种不同的参考核酸杂交，其中所述至少两种不同参考核酸的来源可根据至少一种表型参数的值可分离成至少两个组，两种不同的参考杂交模式， 并通过无监督的多变量分析对参考杂交模式进行聚类。 该方法进一步提供了一种用于分型样品核酸的方法，其包括通过使用根据本发明的方法提供用于样品核酸的至少两个不同的参考杂交模式簇，将用于制备参考杂交模式的相同阵列与 样品核酸以获得样品杂交模式，并将所述样品杂交模式分配给所述至少两个不同的参考杂交模式簇之一。

    Real-time method for the detection of viable micro-organisms
    9.
    发明授权
    Real-time method for the detection of viable micro-organisms 有权
    用于检测可行微生物的实时方法

    公开(公告)号：US08986946B2

    公开(公告)日：2015-03-24

    申请号：US12809566

    申请日：2008-12-19

    申请人： Remco Kort ,  Frank Henri Johan Schuren ,  Roy Christiaan Montijn

    发明人： Remco Kort ,  Frank Henri Johan Schuren ,  Roy Christiaan Montijn

    IPC分类号： C12Q1/18 ,  C12Q1/04 ,  G01N21/64 ,  C12Q1/06 ,  G01N21/80

    CPC分类号： C12Q1/04 ,  G01N21/6428 ,  G01N21/80

    摘要： The invention relates to a method for real-time detection of viable microorganisms comprising: a. addition of a cell-permeable, phototautomeric compound to a micro-organism or other living cell; and b. measuring the fluorescent emission of said phototautomeric compound. Preferably the phototautomeric compound is salicylic acid, 2-hydroxy-1-naphtoic acid or 1-hydroxy-2-naphtoic acid. Further, the assay can he used to assess the antibiotic effect of a test compound. This test can be used as a high—throughput screening for compounds with antibiotic activity. Also part of the invention is the use of a cell permeable phototautomeric compound in a method for determining the viability of micro-organisms and for assessing the antibiotic effect of a test compound.

    摘要翻译： 本发明涉及一种用于实时检测活微生物的方法，包括：a。 向微生物或其他活细胞中加入细胞可渗透的光敏化合物; 和b。 测量所述光自由基化合物的荧光发射。 优选的光敏化合物是水杨酸，2-羟基-1-萘甲酸或1-羟基-2-萘甲酸。 此外，他可以使用该测定来评估测试化合物的抗生素效果。 该测试可用作具有抗生素活性的化合物的高通量筛选。 本发明的一部分也是在测定微生物活力的方法中以及用于评估测试化合物的抗生素效果的方法中使用细胞可渗透的光致敏化合物。