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1.发明授权公开(公告)号：US08728771B2
公开(公告)日：2014-05-20
申请号：US13139205
申请日：2009-12-10
申请人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
发明人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
CPC分类号： C12N9/80 , C12P13/04 , C12P13/06 , C12P13/222 , C12P41/007
摘要： The present invention provides a L-succinylacylase consisting of: (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; or (d) a protein which consists of an amino acid sequence where one or several amino acid(s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity. This enzyme is able to produce a sterically bulky unnatural amino acid such as L-tert-leucine etc. which is useful as an intermediate for pharmaceuticals.
摘要翻译： 本发明提供了一种L-琥珀酰基酰化酶，其由以下组成：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 或（d）由氨基酸序列组成的蛋白质，其中一个或多个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。 该酶能够产生空间庞大的非天然氨基酸如L-叔亮氨酸等，其可用作药物的中间体。
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公开(公告)号：US20150344852A1
公开(公告)日：2015-12-03
申请号：US14367822
申请日：2012-12-19
IPC分类号： C12N9/02
CPC分类号： C12N9/0036 , C12Y106/99
摘要： [Object] To provide: a novel diaphorase; a method for producing the diaphorase; and use of the diaphorase.[Solution] A diaphorase comprising any one of the following polypeptides (a) to (c): (a) a polypeptide having the amino acid sequence of SEQ ID NO: 1, (b) a polypeptide having the amino acid sequence of SEQ ID NO: 1 in which one or several amino acid residues are substituted, deleted, inserted, added, and/or inverted, and having diaphorase activity, and (c) a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having diaphorase activity.
摘要翻译： [目的]提供：一种新颖的心电图仪; 一种生产心肌黄酶的方法; 并使用心肌黄酶。 [解决方案]包含以下多肽（a）至（c）中的任一种的心肌黄酶：（a）具有SEQ ID NO：1的氨基酸序列的多肽，（b）具有SEQ ID NO： 其中一个或几个氨基酸残基被取代，缺失，插入，添加和/或倒转并具有心肌黄酶活性的NO：1，和（c）具有与氨基酸具有80％或更高同一性的氨基酸序列的多肽 酸序列，并具有心肌黄酶活性。
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3.发明授权公开(公告)号：US08470574B2
公开(公告)日：2013-06-25
申请号：US12989728
申请日：2009-05-07
申请人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Takeshi Miyata , Aya Oosato , Shinya Kumagai , Toshihide Yamada
发明人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Takeshi Miyata , Aya Oosato , Shinya Kumagai , Toshihide Yamada
CPC分类号： C12N9/80 , A23L33/175 , C12P13/04 , C12P13/06 , C12P41/007
摘要： The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals.A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.
摘要翻译： 本发明提供能够产生L-叔亮氨酸的L-氨基酸酰基酶，其可用作药物的中间体。 特征在于以下（a）〜（d）中任一项所示的蛋白质：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 和（d）由氨基酸序列组成的蛋白质，其中一个或几个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。
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4.发明申请公开(公告)号：US20110250653A1
公开(公告)日：2011-10-13
申请号：US13139205
申请日：2009-12-10
申请人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
发明人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Shinya Kumagai
CPC分类号： C12N9/80 , C12P13/04 , C12P13/06 , C12P13/222 , C12P41/007
摘要： The present invention provides a L-succinylacylase consisting of: (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; or (d) a protein which consists of an amino acid sequence where one or several amino acid(s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity. This enzyme is able to produce a sterically bulky unnatural amino acid such as L-tert-leucine etc. which is useful as an intermediate for pharmaceuticals.
摘要翻译： 本发明提供了一种L-琥珀酰基酰化酶，其由以下组成：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 或（d）由氨基酸序列组成的蛋白质，其中一个或多个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。 该酶能够产生空间庞大的非天然氨基酸如L-叔亮氨酸等，其可用作药物的中间体。
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5.发明申请公开(公告)号：US20110244530A1
公开(公告)日：2011-10-06
申请号：US12989728
申请日：2009-05-07
申请人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Takeshi Miyata , Aya Oosato , Shinya Kumagai , Toshihide Yamada
发明人： Atsushi Toda , Sachio Iwai , Yoshiaki Nishiya , Takeshi Miyata , Aya Oosato , Shinya Kumagai , Toshihide Yamada
CPC分类号： C12N9/80 , A23L33/175 , C12P13/04 , C12P13/06 , C12P41/007
摘要： The present invention provides an L-aminoacylase which is able to produce L-tert-leucine being useful as an intermediate for pharmaceuticals.A protein which is characterized in being represented by any of the following (a) to (d): (a) a protein coded by a gene consisting of a nucleic acid sequence shown in SEQ ID No: 1; (b) a protein consisting of an amino acid sequence shown in SEQ ID No: 2; (c) a protein coded by a polynucleotide which hybridizes under a stringent condition with a nucleic acid sequence which is complementary to the nucleic acid sequence shown in SEQ ID No: 1 and having an L-succinylaminoacylase activity; and (d) a protein which consists of an amino acid sequence where one or several amino acid (s) is/are substituted, deleted, inserted and/or added in the protein consisting of the amino acid sequence shown in SEQ ID No: 2 and has an L-succinylaminoacylase activity.
摘要翻译： 本发明提供能够产生L-叔亮氨酸的L-氨基酸酰基酶，其可用作药物的中间体。 特征在于以下（a）〜（d）中任一项所示的蛋白质：（a）由由SEQ ID No：1所示的核酸序列组成的基因编码的蛋白质; （b）由SEQ ID No：2所示的氨基酸序列组成的蛋白质; （c）由严格条件下与SEQ ID No：1所示核酸序列互补且具有L-琥珀酰氨基酰化酶活性的核酸序列杂交的多核苷酸编码的蛋白质; 和（d）由氨基酸序列组成的蛋白质，其中一个或几个氨基酸被取代，缺失，插入和/或添加在由SEQ ID No：2所示的氨基酸序列组成的蛋白质中 并具有L-琥珀酰氨基酰化酶活性。
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