DIAPHORASE
    2.
    发明申请

    公开(公告)号:US20150344852A1

    公开(公告)日:2015-12-03

    申请号:US14367822

    申请日:2012-12-19

    IPC分类号: C12N9/02

    CPC分类号: C12N9/0036 C12Y106/99

    摘要: [Object] To provide: a novel diaphorase; a method for producing the diaphorase; and use of the diaphorase.[Solution] A diaphorase comprising any one of the following polypeptides (a) to (c): (a) a polypeptide having the amino acid sequence of SEQ ID NO: 1, (b) a polypeptide having the amino acid sequence of SEQ ID NO: 1 in which one or several amino acid residues are substituted, deleted, inserted, added, and/or inverted, and having diaphorase activity, and (c) a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having diaphorase activity.

    摘要翻译: [目的]提供:一种新颖的心电图仪; 一种生产心肌黄酶的方法; 并使用心肌黄酶。 [解决方案]包含以下多肽(a)至(c)中的任一种的心肌黄酶:(a)具有SEQ ID NO:1的氨基酸序列的多肽,(b)具有SEQ ID NO: 其中一个或几个氨基酸残基被取代,缺失,插入,添加和/或倒转并具有心肌黄酶活性的NO:1,和(c)具有与氨基酸具有80%或更高同一性的氨基酸序列的多肽 酸序列,并具有心肌黄酶活性。

    α1-6 fucosyltransferase
    4.
    发明授权
    α1-6 fucosyltransferase 有权
    α1-6岩藻糖基转移酶

    公开(公告)号:US07264955B2

    公开(公告)日:2007-09-04

    申请号:US10844432

    申请日:2004-05-13

    IPC分类号: C12N9/10 C07H21/04 C12P21/06

    CPC分类号: C12N9/1051

    摘要: An isolated, porcine α1-6 fucosyltransferase having the following physico-chemical properties: (1) action: transferring fucose from guanosine diphosphate-fucose to a hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAcβ1-2Manα1-6)(GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAcβ1-2Manα1-6)-(GlcNAcβ1-2Manα1-3)Manα1-4GlcNAcβ1-4(Fucα1-6)GlucNAc-R (2) optimum pH: about 7.0 (3) pH stability: retains activity after 5 hours of treatment at 4° C. at a pH range of 4.0-10.0 (4) optimum temperature: about 30-37° C. (5) inhibition or activation: no requirement for divalent metal for expression of activity; no inhibition of activity in the presence of 5 mM EDTA (6) molecular weight: about 60,000 by SDS-polyacrylamide gel electrophoresis is provided. An isolated polynucleotide encoding porcine α1-6 fucosyltransferase is also provided. Expression vectors comprising a polynucleotide encoding porcine α1-6 fucosyltransferase are also provided.

    摘要翻译: 具有以下物理化学性质的分离的猪α1-6岩藻糖基转移酶:(1)作用:将岩藻糖从鸟苷二磷酸 - 岩藻糖转移到最接近受体R的GluNAc的6-位的羟基(GlcNAcbeta1-2Manalpha1-6 )(GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlucNAc-R其中R是携带所述残基的天冬酰胺残基或肽链,由此形成(GlcNAcbeta1-2Manalpha1-6) - (GlcNAcbeta1-2Manalpha1-3)Manalpha1-4GlcNAcbeta1 -4(Fucalpha1-6)GlucNAc-R(2)最适pH:约7.0(3)pH稳定性:在4.0〜1.0.0的pH范围内,4℃下处理5小时后保持活性(4)最适温度: 约30-37℃。(5)抑制或活化:不需要二价金属来表达活性; 提供了通过SDS-聚丙烯酰胺凝胶电泳在5mM EDTA(6)分子量:约60,000存在下的活性抑制。 还提供了编码猪α1-6岩藻糖基转移酶的分离的多核苷酸。 还提供了包含编码猪α1-6岩藻糖基转移酶的多核苷酸的表达载体。

    &agr;1-6 fucosyltransferase
    5.
    发明授权
    &agr;1-6 fucosyltransferase 失效
    α1-6岩藻糖基转移酶

    公开(公告)号:US06291219B1

    公开(公告)日:2001-09-18

    申请号:US09442629

    申请日:1999-11-18

    IPC分类号: C12N900

    CPC分类号: C12N9/1051

    摘要: Porcine- or human-derived &agr;1-6 fucosyltransferases having the following action: action: transferring fucose from guanosine diphosphate-fucose to the hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAc&bgr;1-2Man&agr;1-6) (GlcNAc&bgr;1-2Man&agr;1-3) Man&bgr;1-4GlcNAc&bgr;1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc&bgr;1-2Man&agr;1-6)-(GlcNAc&bgr;1-2Man&agr;1-3)Man&bgr;1-4GlcNAc&bgr;1-4(Fuc&agr;1-6)GlucNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant &agr;1-6 fucosyltransferase, by culturing the transformant.

    摘要翻译: 具有以下作用的猪或人来源的α1-6岩藻糖基转移酶:作用:将岩藻糖从鸟苷二磷酸岩藻糖转移到最接近受体R的GluNAc的6位羟基(GlcNAcbeta1-2Manalpha1-6)(GlcNAcβ1- 2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4GlucNAc-R其中R是携带所述残基的天冬酰胺残基或肽链,由此形成(GlcNAcbeta1-2Manalpha1-6) - (GlcNAcbeta1-2Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1 -6)GlucNAc-R; 编码这些酶的基因; 含有该基因的表达载体; 通过使用该表达载体制备的转化体; 以及通过培养转化体来制备重组α1-6岩藻糖基转移酶的方法。

    .alpha.1-6 fucosyltransferase
    6.
    发明授权
    .alpha.1-6 fucosyltransferase 失效
    α1-岩藻糖基转移酶

    公开(公告)号:US6054304A

    公开(公告)日:2000-04-25

    申请号:US913805

    申请日:1998-01-07

    CPC分类号: C12N9/1051

    摘要: Porcine- or human-derived .alpha.1-6 fucosyltransferases having the following action:action: transferring fucose from guanosine adiphosphate-fucose to the hydroxy group at 6-position of GlcNAc closest to R of a receptor (GlcNAc.beta.1-2Man.alpha.1-6)(GlcNAc.beta.1-2Man.alpha.1-3)Man.beta.1-4GlcNAc.beta.1-4GlcNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc.beta.1-2Man.alpha.1-6)-(GlcNAc.beta.1-2Man.alpha.1-3)Man.beta.1-4GlcNAc.beta.1-4(Fuc.alpha.1-6)GlcNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant .alpha.1-6 fucosyltransferase, by culturing the transformant.

    摘要翻译: PCT No.PCT / JP97 / 00171 Sec。 371日期:1998年1月7日 102(e)1998年1月7日PCT PCT 1997年1月23日PCT公布。 公开号WO97 / 27303 日期1997年7月31日具有以下作用的雄激素或人源α1-岩藻糖基转移酶:作用:将岩藻糖从鸟苷酸岩藻糖转移到最接近受体R的GlcNAc的6位的羟基(GlcNAcβ1 -2Manα1-6)(GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlcNAc-R其中R是携带所述残基的天冬酰胺残基或肽链,由此形成(GlcNAcβ1 -2Manα1-6) - (GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4(Fucα1-6)GlcNAc-R; 编码这些酶的基因; 含有该基因的表达载体; 通过使用该表达载体制备的转化体; 以及通过培养转化体来产生重组α1-6岩藻糖基转移酶的方法。