摘要:
Disclosed is a tyrosinase activity controlling agent comprising, as an active ingredient, a compound that has tyrosinase inhibiting or promoting activity, an external preparation comprising the controlling agent, and a process for producing the compound.
摘要:
[Object] To provide: a novel diaphorase; a method for producing the diaphorase; and use of the diaphorase.[Solution] A diaphorase comprising any one of the following polypeptides (a) to (c): (a) a polypeptide having the amino acid sequence of SEQ ID NO: 1, (b) a polypeptide having the amino acid sequence of SEQ ID NO: 1 in which one or several amino acid residues are substituted, deleted, inserted, added, and/or inverted, and having diaphorase activity, and (c) a polypeptide having an amino acid sequence with 80% or more identity to the amino acid sequence of SEQ ID NO: 1, and having diaphorase activity.
摘要翻译:[目的]提供:一种新颖的心电图仪; 一种生产心肌黄酶的方法; 并使用心肌黄酶。 [解决方案]包含以下多肽(a)至(c)中的任一种的心肌黄酶:(a)具有SEQ ID NO:1的氨基酸序列的多肽,(b)具有SEQ ID NO: 其中一个或几个氨基酸残基被取代,缺失,插入,添加和/或倒转并具有心肌黄酶活性的NO:1,和(c)具有与氨基酸具有80%或更高同一性的氨基酸序列的多肽 酸序列,并具有心肌黄酶活性。
摘要:
Disclosed is a tyrosinase activity controlling agent comprising, as an active ingredient, a compound that has tyrosinase inhibiting or promoting activity, an external preparation comprising the controlling agent, and a process for producing the compound.
摘要:
An isolated, porcine α1-6 fucosyltransferase having the following physico-chemical properties: (1) action: transferring fucose from guanosine diphosphate-fucose to a hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAcβ1-2Manα1-6)(GlcNAcβ1-2Manα1-3)Manβ1-4GlcNAcβ1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAcβ1-2Manα1-6)-(GlcNAcβ1-2Manα1-3)Manα1-4GlcNAcβ1-4(Fucα1-6)GlucNAc-R (2) optimum pH: about 7.0 (3) pH stability: retains activity after 5 hours of treatment at 4° C. at a pH range of 4.0-10.0 (4) optimum temperature: about 30-37° C. (5) inhibition or activation: no requirement for divalent metal for expression of activity; no inhibition of activity in the presence of 5 mM EDTA (6) molecular weight: about 60,000 by SDS-polyacrylamide gel electrophoresis is provided. An isolated polynucleotide encoding porcine α1-6 fucosyltransferase is also provided. Expression vectors comprising a polynucleotide encoding porcine α1-6 fucosyltransferase are also provided.
摘要:
Porcine- or human-derived &agr;1-6 fucosyltransferases having the following action: action: transferring fucose from guanosine diphosphate-fucose to the hydroxy group at 6-position of GluNAc closest to R of a receptor (GlcNAc&bgr;1-2Man&agr;1-6) (GlcNAc&bgr;1-2Man&agr;1-3) Man&bgr;1-4GlcNAc&bgr;1-4GlucNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc&bgr;1-2Man&agr;1-6)-(GlcNAc&bgr;1-2Man&agr;1-3)Man&bgr;1-4GlcNAc&bgr;1-4(Fuc&agr;1-6)GlucNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant &agr;1-6 fucosyltransferase, by culturing the transformant.
摘要:
Porcine- or human-derived .alpha.1-6 fucosyltransferases having the following action:action: transferring fucose from guanosine adiphosphate-fucose to the hydroxy group at 6-position of GlcNAc closest to R of a receptor (GlcNAc.beta.1-2Man.alpha.1-6)(GlcNAc.beta.1-2Man.alpha.1-3)Man.beta.1-4GlcNAc.beta.1-4GlcNAc-R wherein R is an asparagine residue or a peptide chain carrying said residue, whereby to form (GlcNAc.beta.1-2Man.alpha.1-6)-(GlcNAc.beta.1-2Man.alpha.1-3)Man.beta.1-4GlcNAc.beta.1-4(Fuc.alpha.1-6)GlcNAc-R; a gene encoding these enzymes; an expression vector containing the gene; a transformant prepared by using this expression vector; and a method for producing a recombinant .alpha.1-6 fucosyltransferase, by culturing the transformant.