公开(公告)号：US07081353B2

公开(公告)日：2006-07-25

申请号：US10221796

申请日：2001-03-20

申请人： Katsuhiko Machida ,  Sadayori Hoshina ,  Takashi Ushida ,  Junichiro Arai ,  Hideo Katayama ,  Chiaki Okumura ,  Yoshihisa Amano

发明人： Katsuhiko Machida ,  Sadayori Hoshina ,  Takashi Ushida ,  Junichiro Arai ,  Hideo Katayama ,  Chiaki Okumura ,  Yoshihisa Amano

IPC分类号： C12M1/34 ,  C12Q1/18

CPC分类号： C12Q1/06

摘要： A method and apparatus wherein measurement current values output from a first oxygen sensor and a second oxygen sensor are time sequentially measured. Each moving average value is calculated from each sequential measurement current value. Each time differential value is calculated from the pair of each calculated moving average values, by least squares approximation. Then, drug susceptibility is measured based upon each calculated time differential value, so that drug the susceptibility measurement is performed quickly or accurately.

摘要翻译： 其中从第一氧传感器和第二氧传感器输出的测量电流值被时间依次测量的方法和装置。 每个移动平均值由每个顺序测量电流值计算。 每次通过最小平方近似计算每个计算的移动平均值对的差分值。 然后，基于每个计算的时间微分值测量药物敏感性，使得药物敏感性测量快速或准确地进行。

公开(公告)号：US07598074B2

公开(公告)日：2009-10-06

申请号：US10804746

申请日：2004-03-19

申请人： Sadayori Hoshina ,  Atsushi Takahashi ,  Noboru Iiyama ,  Hitoshi Inaba

发明人： Sadayori Hoshina ,  Atsushi Takahashi ,  Noboru Iiyama ,  Hitoshi Inaba

IPC分类号： C02F3/34 ,  A62D3/00 ,  A62D3/02 ,  B09B3/00 ,  B09C1/10

CPC分类号： C02F3/34 ,  A62D2101/22 ,  A62D2101/28 ,  C02F3/1273 ,  C02F2101/366 ,  Y02W10/15

摘要： A reaction tank (5) holds crushed cells comprising a pellicle of Bacillus midousuji cultured in the presence of a chlorinated aromatic compound such as dioxins and holds wastewater comprising a contaminated matter such as fly ash which comprises dioxins and which is produced by washing of a facility such as an incinerator. Air is supplied by a blower (6) to a matter held in the reaction tank (5). Accordingly, dioxins having three or more chlorine atoms in the contaminated matter can be decomposed, and the contaminated matter can be easily cleaned at a site where dioxins generate such as a washing or dismantling site of an incineration facility.

摘要翻译： 反应罐（5）容纳破碎的细胞，其包含在氯化芳族化合物如二恶英存在下培养的芽孢杆菌（Bacillus midousuji）的防护薄膜组合物，并且包含含有二氧diox类物质的粉煤灰等污染物质的废水， 如焚烧炉。 空气由鼓风机（6）供应到保持在反应罐（5）中的物质。 因此，污染物质中具有三个以上氯原子的二恶英可以分解，并且污染物质可以在二恶英产生的地点容易地清洗，例如焚烧设备的洗涤或拆除场所。

公开(公告)号：US07198906B2

公开(公告)日：2007-04-03

申请号：US10221800

申请日：2001-03-19

申请人： Katsuhiko Machida ,  Sadayori Hoshina ,  Takashi Ushida ,  Junichiro Arai ,  Hideo Katayama ,  Chiaki Okumura ,  Yoshihisa Amano

发明人： Katsuhiko Machida ,  Sadayori Hoshina ,  Takashi Ushida ,  Junichiro Arai ,  Hideo Katayama ,  Chiaki Okumura ,  Yoshihisa Amano

IPC分类号： C12Q1/18 ,  C12M1/34

CPC分类号： C12Q1/025 ,  C12Q1/18

摘要： Disclosed herein is a method for determining the drug sensitivity of a microbe which comprises pouring a microbial suspension into each of the compartments or wells at a position which is in the vicinity of an electrode for measuring dissolved oxygen concentration, measuring current from each electrode at a second time interval for a third time period, each time is obtained based upon each of the measured currents at which the maximum current is obtained, obtaining each current value within a fourth time period which starts from the time at which the maximum current is obtained, detecting drug sensitivity based upon the variation condition of each current value during the fourth time period. The method allows rapid drug susceptibility measurements.

摘要翻译： 本文公开了一种用于测定微生物药物敏感性的方法，该方法包括将微生物悬浮液在位于用于测量溶解氧浓度的电极附近的位置处的每个隔室或孔中倒入，测量来自每个电极的电流 第三时间段的第二时间间隔，基于获得最大电流的每个测量电流获得每次，获得从获得最大电流的时间开始的第四时间段内的每个电流值， 基于第四时间段内的每个电流值的变化条件来检测药物敏感性。 该方法允许快速的药物敏感性测量。

公开(公告)号：US06420165B1

公开(公告)日：2002-07-16

申请号：US09577640

申请日：2000-05-24

申请人： Bernard I. Weinstein ,  David Figurski ,  Sadayori Hoshina ,  Koji Nakanishi

发明人： Bernard I. Weinstein ,  David Figurski ,  Sadayori Hoshina ,  Koji Nakanishi

IPC分类号： B09B300

CPC分类号： C12R1/07 ,  C02F3/34 ,  Y10S435/832

摘要： A biologically pure culture of a microorganism is provided designated SH2A and deposited under ATCC Accession No. 55926, or a mutant derived therefrom. Further provided is a biologically pure culture of a microorganism designated SH2B and deposited under ATCC Accession No. 202050, or a mutant derived therefrom. A method of degrading an organic material such as sludge is carried out by treating the organic material with an effective, degrading amount of either SH2A or a mutant derived therefrom, or SH2B or a mutant derived therefrom. The microorganism designated SH2A or a mutant derived therefrom, or SH2B or a mutant derived therefrom, is grown by culturing the microorganism at a temperature and in a medium effective to promote growth of the microorganism.

摘要翻译： 提供微生物的生物学纯培养物，命名为SH2A，保藏于ATCC保藏号55926，或由其衍生的突变体。 进一步提供了一种生物学纯的指定为SH2B并保藏于ATCC保藏号为202050的微生物或其衍生的突变体的培养物。 通过用有效降解量的SH2A或其衍生的突变体或SH2B或其衍生的突变体处理有机材料来进行有机材料如污泥的降解方法。 通过在有效促进微生物生长的温度和介质中培养微生物来生长称为SH2A或其衍生物的突变体或SH2B或其衍生的突变体。

公开(公告)号：US06190903B1

公开(公告)日：2001-02-20

申请号：US08979586

申请日：1997-11-26

申请人： I. Bernard Weinstein ,  David Figurski ,  Sadayori Hoshina ,  Koji Nakanishi

发明人： I. Bernard Weinstein ,  David Figurski ,  Sadayori Hoshina ,  Koji Nakanishi

IPC分类号： B09B300

CPC分类号： C12R1/07 ,  C02F3/34 ,  Y10S435/832

摘要： A biologically pure culture of a microorganism is provided designated SH2A and deposited under ATCC Accession No. 55926, or a mutant derived therefrom. Further provided is a biologically pure culture of a microorganism designated SH2B and deposited under ATCC Accession No. 202050, or a mutant derived therefrom. A method of degrading an organic material is carried out by treating the organic material with an effective, degrading amount of either SH2A or a mutant derived therefrom, or SH2B or a mutant derived therefrom. The microorganism designated SH2A or a mutant derived therefrom, or SH2B or a mutant derived therefrom, is grown by culturing the microorganism at a temperature and in a medium effective to promote growth of the microorganism.

摘要翻译： 提供微生物的生物学纯培养物，命名为SH2A，保藏于ATCC保藏号55926，或由其衍生的突变体。 进一步提供了指定为SH2B并保藏于ATCC保藏号202050下的微生物的生物学纯培养物，或由其衍生的突变体。 通过用有效降解量的SH2A或由其衍生的突变体或SH2B或其衍生的突变体处理有机材料来进行有机材料的降解的方法。 通过在有效促进微生物生长的温度和介质中培养微生物来生长称为SH2A或其衍生物的突变体或SH2B或其衍生的突变体。

公开(公告)号：US20050208642A1

公开(公告)日：2005-09-22

申请号：US10804746

申请日：2004-03-19

申请人： Sadayori Hoshina ,  Atsushi Takahashi ,  Noboru Iiyama ,  Hitoshi Inaba

发明人： Sadayori Hoshina ,  Atsushi Takahashi ,  Noboru Iiyama ,  Hitoshi Inaba

IPC分类号： A62D101/22 ,  A62D101/28 ,  C02F3/12 ,  C02F3/34 ,  C12N1/20 ,  C12S1/00

CPC分类号： C02F3/34 ,  A62D2101/22 ,  A62D2101/28 ,  C02F3/1273 ,  C02F2101/366 ,  Y02W10/15

摘要： A reaction tank (5) holds crushed cells comprising a pellicle of Bacillus midousuji cultured in the presence of a chlorinated aromatic compound such as dioxins and holds wastewater comprising a contaminated matter such as fly ash which comprises dioxins and which is produced by washing of a facility such as an incinerator. Air is supplied by a blower (6) to a matter held in the reaction tank (5). Accordingly, dioxins having three or more chlorine atoms in the contaminated matter can be decomposed, and the contaminated matter can be easily cleaned at a site where dioxins generate such as a washing or dismantling site of an incineration facility.

摘要翻译： 反应罐（5）容纳破碎的细胞，其包含在氯化芳族化合物如二恶英存在下培养的芽孢杆菌（Bacillus midousuji）的防护薄膜组合物，并且包含含有二氧diox类物质的粉煤灰等污染物质的废水， 如焚烧炉。 空气由鼓风机（6）供应到保持在反应罐（5）中的物质。 因此，污染物质中具有三个以上氯原子的二恶英可以分解，并且污染物质可以在二恶英产生的地点容易地清洗，例如焚烧设备的洗涤或拆除场所。

公开(公告)号：US5571674A

公开(公告)日：1996-11-05

申请号：US227475

申请日：1994-04-14

申请人： Sadayori Hoshina ,  I. Bernard Weinstein

发明人： Sadayori Hoshina ,  I. Bernard Weinstein

IPC分类号： C07K14/205 ,  C12Q1/68 ,  C12P19/34

CPC分类号： C07K14/205 ,  C12Q1/689

摘要： This invention provides a DNA oligomer having the sequence 5'GGACATAGGCTGATCTCTTAGC3' (SEQ ID NO: 1) and which is complementary to Campylobacter pylori 16S ribosomal RNA sequences, for use as a probe to detect Campylobacter pylori.This invention also provides DNA oligomers having the sequences 5'GCGCAATCAGCGTCAGGTAATG3' (SEQ ID NO: 2) and 5'GCTAAGAGATCAGCCTATGTCCC3' (SEQ ID NO: 3) and which are complementary to certain Campylobacter pylori 16S ribosomal RNA sequences, for use as polymerase chain reaction primers for the detection of Campylobacter pylori.This invention also provides a method for producing species-specific bacterial or protozoan DNA oligomers encoding 16S ribosomal RNA by means of the polymerase chain reaction for use as species-specific probes and PCR primers, and methods for detection and identification of bacteria and protozoa.Further, this invention provides a DNA oligomer having the sequence 5'ACGGGCGGTGTGTGC3' (SEQ ID NO: 4).

摘要翻译： 本发明提供具有序列5'GGACATAGGCTGATCTCTTAGC3'（SEQ ID NO：1）并与幽门螺杆菌16S核糖体RNA序列互补的DNA寡聚体，用作检测幽门螺杆菌的探针。 本发明还提供具有序列5'GCGCAATCAGCGTCAGGTAATG3'（SEQ ID NO：2）和5'GCTAAGAGATCAGCCTATGTCCC3'（SEQ ID NO：3）的DNA寡聚体，它们与某些幽门螺杆菌16S核糖体RNA序列互补，用作聚合酶链 用于检测幽门螺杆菌的反应引物。 本发明还提供了通过用作物种特异性探针和PCR引物的聚合酶链反应来生产编码16S核糖体RNA的物种特异性细菌或原生动物DNA寡聚体的方法，以及用于检测和鉴定细菌和原生动物的方法。 此外，本发明提供具有5'ACGGGGGGGTGTGTGC3'（SEQ ID NO：4）序列的DNA寡聚体。