公开(公告)号：US5556760A

公开(公告)日：1996-09-17

申请号：US381000

申请日：1995-01-31

申请人： Michihiro Nakamura ,  Satomi Matsui ,  Keiko Oka ,  Hitoshi Tsuruta ,  Yoshihiro Koori ,  Takuji Kato ,  Shigeji Ito

发明人： Michihiro Nakamura ,  Satomi Matsui ,  Keiko Oka ,  Hitoshi Tsuruta ,  Yoshihiro Koori ,  Takuji Kato ,  Shigeji Ito

IPC分类号： G01N27/416 ,  A61B10/00 ,  C12M1/34 ,  C12Q1/00 ,  C12Q1/58 ,  A61B5/04

CPC分类号： A61B5/14539 ,  C12Q1/00 ,  C12Q1/58

摘要： A method for assaying an enzyme on a targeted solid body surface, including the steps of contacting an end opening of a thin tube with a targeted solid body surface to form a space between the targeted solid body surface and inner wall surface of the thin tube, supplying a substrate solution into the space to bring the substrate solution in contact with the targeted solid body surface, and determining a pH change of the substrate solution in the space by using a pH electrode; and an apparatus therefor.

摘要翻译： 一种用于测定目标实体表面上的酶的方法，包括以下步骤：使细管的端部开口与目标实体表面接触，以在目标实体表面和细管的内壁表面之间形成空间， 将基材溶液供给到所述空间中，使所述基材溶液与所述目标固体表面接触，并且使用pH电极确定所述空间中的所述基材溶液的pH变化; 及其装置。

公开(公告)号：US5674696A

公开(公告)日：1997-10-07

申请号：US328057

申请日：1994-10-24

申请人： Michihiro Nakamura ,  Satomi Matsui ,  Keiko Oka ,  Hitoshi Tsuruta

发明人： Michihiro Nakamura ,  Satomi Matsui ,  Keiko Oka ,  Hitoshi Tsuruta

IPC分类号： G01N33/53 ,  G01N27/416 ,  G01N33/543 ,  G01N27/327 ,  G01N33/569

CPC分类号： G01N27/4145 ,  Y10S435/817 ,  Y10S436/806

摘要： There is disclosed an apparatus for the determination of a trace amount of an analyte substance or organism. A substrate solution is brought into contact with a small diameter tube which has captured, at least on its inner surface, a trace amount of an analyte substance or organism. The trace amount has the ability to change the pH of the substrate solution. A pH change following a reaction of a substrate in the substrate solution in the small diameter tube is measured; the apparatus has a cuvette having an inlet and an outlet for the substrate solution, a pH electrode accomodated in the cuvette, a pump for supplying the substrate solution in the cuvette, a positioning structure for positioning, in the cuvette, the small diameter tube with the pH sensitive plane of the pH electrode and spacing the surface and the plane at 1 mm or below from each other, and a sealing structure for maintaining a formed by the outer surface of the small diameter tube accommodated in the cuvette and the inner surface of the cuvette at liquid tight condition.

摘要翻译： 公开了一种用于分析物物质或生物体的痕迹量的测定的装置。 将底物溶液与小直径管接触，其至少在其内表面捕获痕量的被分析物质或生物体。 痕量具有改变的底物溶液的pH的能力。 测量小直径管中底物溶液中底物反应后的pH变化; 该装置具有一个具有用于底物溶液的入口和出口的比色杯，一个容纳在该比色皿中的pH电极，一个用于将该基质溶液供应到该比色杯中的泵，一个定位结构，用于在该小杯中定位该小直径管， pH电极的pH敏感平面，并将表面和平面彼此间隔1mm或1mm以下，并且保持由容纳在反应杯中的小直径管的外表面形成的密封结构， 液体条件下的比色杯。