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    MASS SPECTROMETRY SYSTEM AND MASS SPECTROMETRY METHOD
    2.
    发明申请
    MASS SPECTROMETRY SYSTEM AND MASS SPECTROMETRY METHOD 失效
    质谱系统和质谱方法

    公开(公告)号：US20080283740A1

    公开(公告)日：2008-11-20

    申请号：US12038829

    申请日：2008-02-28

    申请人： Shuhei HASHIBA ,  Takeshi Sakamoto

    发明人： Shuhei HASHIBA ,  Takeshi Sakamoto

    IPC分类号： B01D59/44

    CPC分类号： H01J49/0027 ,  G01N30/724 ,  Y10T436/13

    摘要： When a liquid mixture of two samples such as biological samples labeled with stable isotopes is subjected to a relative quantitative analysis using a liquid chromatography-tandem mass spectrometry system, various constituents are simultaneously ionized. Accordingly, sufficient time required for second mass spectrometry is not ensured, whereby some ions remain unanalyzed after measurement. To address this problem, after second mass spectrometry, amino acid sequencing is performed using the analysis data of the second mass spectrometry, which enables determination on the presence/absence of a specific amino acid labeled with a stable isotope. When the specific amino acid is present, the m/z value of an isotopically-labeled-paired ion in an MS spectrum is calculated, and non-target information for use in second mass spectrometry is created using the calculated m/z information. This avoids redundant second mass spectrometry on sample components derived from the same peptide while allowing second mass spectrometry to be efficiently performed.

    摘要翻译： 当使用液相色谱 - 串联质谱系统进行相对定量分析，将诸如用稳定同位素标记的生物样品的两个样品的液体混合物进行相对定量分析时，同时电离各种成分。 因此，不能确保第二质谱所需的足够的时间，从而在测量之后一些离子保持未分析。 为了解决这个问题，在第二次质谱法之后，使用第二质谱分析数据进行氨基酸测序，其能够确定用稳定同位素标记的特定氨基酸的存在/不存在。 当存在特定氨基酸时，计算MS谱中同位素标记配对离子的m / z值，并使用所计算的m / z信息产生用于第二质谱的非目标信息。 这避免了来自相同肽的样品组分的冗余第二质谱，同时允许有效地进行第二质谱。

    Method for selecting oocytes by using a response to LPA stimulation
    5.
    发明申请
    Method for selecting oocytes by using a response to LPA stimulation 审中-公开
    通过使用对LPA刺激的反应来选择卵母细胞的方法

    公开(公告)号：US20050084839A1

    公开(公告)日：2005-04-21

    申请号：US10876551

    申请日：2004-06-28

    申请人： Tomoko Takeshita ,  Takako Mizoguchi ,  Shuhei Hashiba ,  Jun Otomo

    发明人： Tomoko Takeshita ,  Takako Mizoguchi ,  Shuhei Hashiba ,  Jun Otomo

    IPC分类号： C12N15/09 ,  A01N1/02 ,  C12N5/00 ,  C12N5/075 ,  C12N5/10 ,  C12Q1/02

    CPC分类号： C12N5/0612 ,  C12N5/0609

    摘要： The selection of oocytes has largely relied on manual procedures, resulting in their lack of uniformity. Groups of oocytes are prepared and sorted, the current responses of these groups of oocytes upon exposure to lysophosphatidic acid (LPA) are measured, the response of G protein-coupled receptor (GPCR) is determined for each group of oocytes, and desirable groups of oocytes are selected on the basis of the response of GPCR. In this way, oocytes having a uniformly high response of GPCR can be selected.

    摘要翻译： 卵母细胞的选择在很大程度上取决于手工程序，导致其缺乏一致性。 卵母细胞的组成和分选，测定了这些卵母细胞群暴露于溶血磷脂酸（LPA）后的当前反应，测定每组卵母细胞G蛋白偶联受体（GPCR）的反应， 基于GPCR的反应选择卵母细胞。 以这种方式，可以选择具有GPCR均匀高响应的卵母细胞。

    Mass spectrometer and mass analyzing method
    6.
    发明授权
    Mass spectrometer and mass analyzing method 有权
    质谱仪和质量分析方法

    公开(公告)号：US09184037B2

    公开(公告)日：2015-11-10

    申请号：US13527627

    申请日：2012-06-20

    申请人： Shun Kumano ,  Masuyuki Sugiyama ,  Yuichiro Hashimoto ,  Hideki Hasegawa ,  Masuyoshi Yamada ,  Hidetoshi Morokuma ,  Shuhei Hashiba

    发明人： Shun Kumano ,  Masuyuki Sugiyama ,  Yuichiro Hashimoto ,  Hideki Hasegawa ,  Masuyoshi Yamada ,  Hidetoshi Morokuma ,  Shuhei Hashiba

    IPC分类号： H01J49/00 ,  H01J49/04

    CPC分类号： H01J49/0409 ,  H01J49/0495

    摘要： A mass spectrometer including a sample attaching member of attaching a sample, an ionizing chamber including an introductory port of the sample attaching member and an ionization source of generating a sample ion, a vacuumed chamber having a mass analyzer of analyzing the sample ion, and an opening/closing mechanism provided between the ionizing chamber and the vacuumed chamber, in which the opening/closing mechanism is controlled from a closed state to an open state after introducing the sample attaching member into the ionizing chamber to thereby enable to perform ionization with inconsiderable fragmentation at a high sensitivity with a high throughput.

    摘要翻译： 一种质谱仪，包括附着样品的样品附着构件，包括样品附着构件的入口的电离室和产生样品离子的离子源，具有分析样品离子的质量分析器的真空室，以及 设置在离子化室和真空室之间的开/关机构，其中将样品附着构件引入电离室之后，将开/关机构从关闭状态控制到打开状态，从而能够以不可分割的碎片进行电离 灵敏度高，吞吐量高。

    Mass spectrometry system and mass spectrometry method
    7.
    发明授权
    Mass spectrometry system and mass spectrometry method 失效
    质谱系统和质谱法

    公开(公告)号：US07626162B2

    公开(公告)日：2009-12-01

    申请号：US12038829

    申请日：2008-02-28

    申请人： Shuhei Hashiba ,  Takeshi Sakamoto

    发明人： Shuhei Hashiba ,  Takeshi Sakamoto

    IPC分类号： B01D59/44 ,  H01J49/26 ,  H01J49/42

    CPC分类号： H01J49/0027 ,  G01N30/724 ,  Y10T436/13

    摘要： When a liquid mixture of two samples such as biological samples labeled with stable isotopes is subjected to a relative quantitative analysis using a liquid chromatography-tandem mass spectrometry system, various constituents are simultaneously ionized. Accordingly, sufficient time required for second mass spectrometry is not ensured, whereby some ions remain unanalyzed after measurement. To address this problem, after second mass spectrometry, amino acid sequencing is performed using the analysis data of the second mass spectrometry, which enables determination on the presence/absence of a specific amino acid labeled with a stable isotope. When the specific amino acid is present, the m/z value of an isotopically-labeled-paired ion in an MS spectrum is calculated, and non-target information for use in second mass spectrometry is created using the calculated m/z information. This avoids redundant second mass spectrometry on sample components derived from the same peptide while allowing second mass spectrometry to be efficiently performed.

    摘要翻译： 当使用液相色谱 - 串联质谱系统进行相对定量分析，将诸如用稳定同位素标记的生物样品的两个样品的液体混合物进行相对定量分析时，同时电离各种成分。 因此，不能确保第二质谱所需的足够的时间，从而在测量之后一些离子保持未分析。 为了解决这个问题，在第二次质谱法之后，使用第二质谱分析数据进行氨基酸测序，其能够确定用稳定同位素标记的特定氨基酸的存在/不存在。 当存在特定氨基酸时，计算MS谱中同位素标记配对离子的m / z值，并使用所计算的m / z信息产生用于第二质谱的非目标信息。 这避免了来自相同肽的样品组分的冗余第二质谱，同时允许有效地进行第二质谱。

    MASS SPECTROMETER AND MASS ANALYZING METHOD
    8.
    发明申请
    MASS SPECTROMETER AND MASS ANALYZING METHOD 有权
    质谱仪和质谱分析方法

    公开(公告)号：US20120326022A1

    公开(公告)日：2012-12-27

    申请号：US13527627

    申请日：2012-06-20

    申请人： Shun KUMANO ,  Masuyuki Sugiyama ,  Yuichiro Hashimoto ,  Hideki Hasegawa ,  Masuyoshi Yamada ,  Hidetoshi Morokuma ,  Shuhei Hashiba

    发明人： Shun KUMANO ,  Masuyuki Sugiyama ,  Yuichiro Hashimoto ,  Hideki Hasegawa ,  Masuyoshi Yamada ,  Hidetoshi Morokuma ,  Shuhei Hashiba

    IPC分类号： H01J49/04

    CPC分类号： H01J49/0409 ,  H01J49/0495

    摘要： A mass spectrometer including a sample attaching member of attaching a sample, an ionizing chamber including an introductory port of the sample attaching member and an ionization source of generating a sample ion, a vacuumed chamber having a mass analyzer of analyzing the sample ion, and an opening/closing mechanism provided between the ionizing chamber and the vacuumed chamber, in which the opening/closing mechanism is controlled from a closed state to an open state after introducing the sample attaching member into the ionizing chamber to thereby enable to perform ionization with inconsiderable fragmentation at a high sensitivity with a high throughput

    摘要翻译： 一种质谱仪，包括附着样品的样品附着构件，包括样品附着构件的入口的电离室和产生样品离子的离子源，具有分析样品离子的质量分析器的真空室，以及 设置在离子化室和真空室之间的开/关机构，其中将样品附着构件引入电离室之后，将开/关机构从关闭状态控制到打开状态，从而能够以不可分割的碎片进行电离 灵敏度高，吞吐量高